Imaging Science in Dentistry

Korean Academy of Oral and Maxillofacial Radiology (대한영상치의학회)
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Effect of radiation dose variation on expression of caspase-3 in rat submandibular glands

방사선 조사선량에 따른 백서 악하선의 caspase-3 발현양상

  • Kwon Ki-Jeong (Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University) ;
  • Choi Yong-Suk (Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University) ;
  • Hwang Eui-Hwan (Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University) ;
  • Lee Sang-Rae (Department of Oral and Maxillofacial Radiology, College of Dentistry, Kyung Hee University) ;
  • Koh Kwang-Joon (Department of Oral Maxillofacial Radiology, School of Dentistry, and Institute Science, Chonbuk National University)
  • 권기정 (경희대학교 치과대학 구강악안면방사선학교실) ;
  • 최용석 (경희대학교 치과대학 구강악안면방사선학교실) ;
  • 황의환 (경희대학교 치과대학 구강악안면방사선학교실) ;
  • 이상래 (경희대학교 치과대학 구강악안면방사선학교실) ;
  • 고광준 (전북대학교 치과대학 구강악안면방사선학교실 및 구강생체과학연구소)
  • Published : 2006.03.01
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Abstract

Purpose : To investigate the caspase-3 expression in the acinar and ductal cells of rat submandibular glands after the irradiation of various doses. Materials and Methods : The male Sprague-Dawley rats weighing approximately 250 gm were used for this study. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. The rats were sacrificed on the 1st, 3rd, 7th, 14th, 21 st, and 28th day after irradiation. The specimens including the submandibular gland were sectioned and observed using histopathological and immunohistochemical methods. Results : The local destruction of the acinar and ductal cells and the karyopyknotic nuclei of the acinar cells were observed in the 2 Gy and 5 Gy irradiation groups later than in the 10 Gy and 15 Gy irradiation groups. And the expression of caspase-3 was prominent only in the ductal cells in the 2 Gy and 5 Gy irradiation groups. Conclusion : This experiment suggests that radiation-induced apoptosis in the ductal cells of rat submandibular glands was induced by a low dose radiation associated with the activation of caspase-3 and radiation-induced necrosis was induced by a high dose radiation.

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