PCR-Based Detection and Molecular Genotyping of Enterotoxigenic Clostridium perfringens Isolates from Swine Diarrhea in Korea

  • Kim Sang-Bum (Agriproduct Processing Division, National Rural Resources Development Institute, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Lim Hyeong-Jun (College of Veterinary Medicine, Chungbuk National University) ;
  • Lee Wan-Kyu (College of Veterinary Medicine, Chungbuk National University) ;
  • Hwang In-Gyun (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Woo Gun-Jo (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Ryu Sang-Ryeol (Department of Food and Animal Biotechnology, School of Agricultural Biotechnology, Center for Agricultural Biomaterials, Seoul National University)
  • Published : 2006.02.01

Abstract

Clostridium perfringens strains were isolated from swine diarrhea in Korea. Three out of nineteen (15.8%) isolates of C. perfringens were found to be enterotoxigenic by PCR analysis. PCR-based genotyping of the three enterotoxigenic isolates of C. perfringens revealed that they were types A, C and D, respectively. These results suggest that various types of enterotoxigenic C. perfringens can cause swine diarrhea, and that the presence of enterotoxigenic type A strain, known to be strongly associated with food poisoning, may cause public health problem in Korea.

Keywords

References

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