Condition of Storage and Growth Characteristics on Bulb Propagation of Nerine by Tissue Culture

네리네 대량증식을 위한 자구 저장 및 순화재배

  • Han Soo-Gon (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Kang Chan-Ho (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Lim Hoi-Chun (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Ko Bok-Rai (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Choi Joung-Sik (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Lee Wang-Hyu (Department of Agricultural Biology, Chonbuk National University) ;
  • Choi In-Young (Jeollabuk-do Agricultural Research and Extension Services)
  • Published : 2006.02.01

Abstract

Nerine was originated from South Africa and around 30 kinds of species are distributed in worldwide. In present study we have characterized the condition of storage and growing conditions on the bulb propagation of Nerine by the tissue culture. Result indicated that the percentage of decrement, decay and sprouting of bulblets in the tissue culture was high at the higher temperature compare to lower temperature. The optimum conditions for storage and time were 12 weeks at $15^{\circ}C$ which were having 86.4% sprouting rate. The sprouting rate at different temperature and acclimation time, using bulblets in the tissue culture, were optimum at early June when temperature is relatively higher($22^{\circ}C$) that facilitate to breaking the dormancy. Growth characteristics and capacity of photosynthesis of Nerine were better at red and blue mixed lightness.

Nerine 대량증식을 위한 자구 저장 및 순화재배 조건 등에 관한 연구를 수행하였다. 저장온도와 기간별 자구 무게 참모율은 저온보다 고온에서 증가하였다. 따라서 조직배양 자구의 적정 저장온도와 기간은 86.4%의 출현율을 보이는 $15^{\circ}C$에서 12주가 적절하였다. 또한 기내 생산 자구의 적정토양순화 시기는 휴면타파온도인 $22^{\circ}C$이상 유지가 가능한 6월 상순이 적절하였다. 순화 재배시 효율성 향상을 위한 적정 광원은 적색과 청색의 혼합파장에서 네리네 생육과 광합성 능력이 우수하였다.

Keywords

References

  1. Berghoef, J. and G. Van Brenk. 1983. Effect of temperature on flowering of Nerine bowdenii W. Watts. Acta Horticultureae, 134 : 41-48
  2. De Lint, P.J.A.L. 1969. Flowering in freesia : temperature and corms. Acta Hort. 14 : 125-131
  3. Fortanier, E.J., G. Van Brenk and S.J. Wellensiek. 1979. Growth and flowering of Nerine flexuosa alba. Scientia Horticultureae, 11 : 281- 290 https://doi.org/10.1016/0304-4238(79)90011-6
  4. Lee, S.W. 1974. Studies on dormancy of korean local garlics. J. Kor. Soc. Hort. Sci. 15 : 119-141
  5. Lilien-Kipnis, H., M. Ziv and S. Kahany. 1991. Proliferation and plantlet regeneration from inflorescence-derived Nerine explants cultures in liquid medium. In : J de Jong, Integration of in vitro techniques in ornamental plant breeding. Proceedings of Symposium, 10-14 November 1990, Eucarpia, Section Ornamentals. pp. 28-33
  6. Moon W., B.Y. Lee and J.K. Kim. 1984. Studies on the rest physiology of garlic. II. Effect of temperature on the growth of sprout leaf of clove during storage. J. Kor. Hort. Sci. 25 : 17-22
  7. Paek, K.Y. 1987. Reestablishment in soil of high frequency of apical meristem culture derived microplants of Allium sativum. Resrept RDA (agri Institusional Cooperation), pp. 71-80
  8. Paek, K.Y. and T.A. Thorpe. 1990. Hyacinth. in : Evans DA, Sharp WR, Ammirato PV, Bajaj YPS(eds), pp. 279-508. Handbook of plant Cell Culture Vol 5. macmillan New York
  9. Pierik, R.L.M. and H.H.M. Srttgmans. 1986. Adventitious plantlet regeneration from floral stem explants of Nerine bowdenii W. Watts. Netherlands Journal of Agricultural Science. 34 : 217-223
  10. Rehnstrom, F. 1966. The growth substance content of dormant and of non dormant freesia corms. Copenhagen, K. Veterinaer-OG Landbojskole. Aarsskvift. pp. 148-156
  11. Smee, S. 1984. Growing and breeding Nerines. The Garden. 109 : 408- 413
  12. Sytsema, W. 1975. Flowering and bulb growth of Nerine bowdenii. Acta Horticulturae, 47 : 241-249
  13. Traub, H.P. 1967. Review of the genus Nerine. Plant Life. 23 : 1-23
  14. Tsukamoto, Y. 1973. Dormancy of bulbous crops. Chem. Reg. of Plant. 8 : 21-30