동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제20권1호
  • /
  • Pages.103-114
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  • 2006
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  • 1738-7698(pISSN)
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  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

수종의 한약 복합 추출물의 항균, 항염 및 항알레르기 효과에 대한 실험적 연구

Study on the Anti-microbacterial Activity, Anti-inflammatory and Anti-allergic Effects of Several Herb-Extract

  • 김진만 (원광대학교 한의과대학 한방안이비인후피부과) ;
  • 오한철 (원광대학교 한의과대학 한방안이비인후피부과) ;
  • 송성필 (원광대학교 한의과대학 한방안이비인후피부과) ;
  • 김남권 (원광대학교 한의과대학 한방안이비인후피부과) ;
  • 황충연 (원광대학교 한의과대학 한방안이비인후피부과)
  • Kim Jin-Man (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University) ;
  • Oh Han-Cheol (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University) ;
  • Song Seong-Pil (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Nam-Kwen (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University) ;
  • Hwang Chung-Yeon (Department of Ophthalmology & Otorhinolaryngology & Dermotology, College of Oriental Medicine, Wonkwang University)
  • 발행 : 2006.02.01
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초록

Herbal mixture water extract of (Chrysanthemum morifolium, Portulaca oleracea, Sanguisorba officinalis, Sophora flavescens, Phellodendron amurense, Cnidium monnieri) which exhibit several beneficial effects including acne and skin diseases, was tested for anti-microbial activity and anti-inflammation effects. The herbal mixture extract showed antimicrobial activity against Stapylococcus epidermis, and Propionibacterium acne. The growth of Stapylococcus epidermis, and Propionibacterium acne was inhibited completely by addition of 1.0% of the extract. Also in the present study we examined the mixture extract on compound 48/80 induced allergy and LPS induced cyclooxygenase-2(COX-2) gene expression in RAW 264.7 macrophage. The results indicated the ear swelling and histamine release induced by compound 48/80 were dose-dependently reduced, ranging 28-60%, and 48-72% , respectively. Furthermore the extract inhibited the expression of LPS-induced COX-2 proteins and mRNAs without an appreciable cytotoxic effects on RAW 264.7 cells. The LPS-induced cytokine gene expression including IL-$1{\beta}$, TNF-$\alpha$, and IL-6 were dose-dependently suppressed by the mixture extract. Based on these results, it is concluded that the herbal mixture water extract can be applied to the acne and skin diseases therapy.

키워드

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