Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation of Bacteria Producing a B-Cell-Specific Biological Response Modifier Found in Korean Fermented Soybean Paste

  • CHUNG KUN SUB (Department of Biological Resources and Technology, Yonsei University) ;
  • KIM JOO YOUNG (Department of Microbiology, Yonsei University College of Medicine, Institute of Allergy, Yonsei University College of Medicine, Institute for Immunology, Immunological Diseases, Yonsei University College of Medicine) ;
  • HONG SUNG WOOK (Department of Biological Resources and Technology, Yonsei University) ;
  • LEE BONG KI (Department of Microbiology, Yonsei University College of Medicine, Institute of Allergy , Yonsei University College of Medicine, Institute for Immunology, Immunological Diseases, Yonsei University College of Medicine)
  • Published : 2006.01.01
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Abstract

In a previous study, a biological response modifier (BRM) specifically enhancing the function of B-cells was isolated from Korean fermented soybean paste (Kfsp), but not from non-fermented soybeans. In this study, we attempted to isolate the bacteria producing the BRM from Kfsp (KfspBRM) by ELISA using anti-KfspBRM and by B-cell proliferation. Five bacteria whose culture supernatants showed the BRM activities were isolated, and one of them was identified as Bacillus licheniformis E1. The bacterial BRM (bBRM) originated from a slime layer of B. licheniformis El had a molecular weight of 1,594 kDa, and contained $33\%\;(w/w)$ of reduced sugar and $4.6\%\;(w/w)$ of protein content. The bBRM appeared to be a glycoprotein that is physically, structurally, and functionally similar to the KfspBRM, suggesting that the isolates including B. licheniformis El may produce the KfspBRM in the fermentation process of soybean paste. The mass production of the BRM by the bacterium may help to study B-cells in immunology, and the enrichment of the BRM in Kfsp may help patients in future who are medically in need of potentiation of B-cell proliferation and antibody production.

Keywords

References

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