생명과학회지 (Journal of Life Science)

  • 제16권1호
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  • Pages.120-125
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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과메기에서 histamine 분해능을 나타내는 세균의 분리 동정

Isolation and Identification of Histamine Degrading Bacteria from Kwamegi

  • 김민우 (동의대학교 식품영양학과) ;
  • 김영만 (동의대학교 식품영양학과)
  • Kim Min-Woo (Department of Food Science and Nutrition, Dong-eui University) ;
  • Kim Young-Man (Department of Food Science and Nutrition, Dong-eui University)
  • 발행 : 2006.02.01
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초록

과메기에서 histamine분해능을 가진 세균을 분리 동정하기 위하여 histamine만을 첨가한 제한배지에서 균을 분리했다. 기본적인 형태 및 생화학적 동정시험을 거쳐 10종의 시험균주를 선택하여 16S rDNA 염기서열 비교에 의한 계통발생학적 분석으로 동정하였다. 동정된 세균의 histamine분해능은 탁도측정법과 효소법에 의해 재 확인하였으며 그 실험 결과는 다음과 같다. 16S rDNA 염기서열 비교에 의한 계통 발생학적 분석을 이용하여 동정한 결과, E americana B791, Arthrobacter sp. R45S, H. marisflava, P. sp. 9B-7, Bacillus sp. LMG 21002, P. cibarius JG-220, B. megaterium KL-197 7종이 동정되었고, 각각 $99\%,\;95\%,\;98\%,\;99\%,\;99\%,\;99\%,\;98\%$의 상동성을 보였으며, 3종은 미동정되었다. 동정된 세균의 histamine분해능을 탁도측정법과 효소법에 의해 재 확인한 결과, Arthrobacter sp. R45S, H. marisflava, Bacillus sp. LMG 21002, B. megaterium KL-197이 분해능을 나타내었고, Psychrobacter sp. 9B-7은 미약한 분해능을 보였으며 미동정된 3종도 분해능이 있는 것으로 나타났다. 그리고 E. americana B791과 P. cibarius JG-220은 분해능이 불확실한 것으로 확인되었다 자반고등어에서 분리한 균주보다 시험균주 모두가 생육과 histamine의 분해속도가 비교적 빨랐다.

To isolate and identify histamine degrading bacteria from Kwamegi, bacteria were screened with restriction media containing histamine. Ten strains were selected through morphological and biochemical identification procedure followed by comparison with DNA sequence of 16 rRNA gene. And also, these strains were confirmed by the histamine degrading assay such as turbidity and enzymatic assay. The results of identification are as followings : Ewingella americana B791, Arthrobacter sp. R45S, Halomonas marisflava, Psychrobacter sp. 9B-7, Bacillus sp. LMC 21002, Psychrohacter cibarius BC-220, Bacillus megaterium KL-197 were identified showing homology of $99\%,\;95\%,\;98\%,\;99\%,\;99\%,\;99\%\;and\;98\%$, respectively. Three strains remain unidentified. Arthrobacter sp. R45S, H. marisflava, Bacillus sp. LMG 21002, B. megaterium KL-197 showed histamine degrading activity, whereas, Psychrobacter sp. 9B-7 only showed weak activity. Three unidentified strains also have histamine degrading activity. In contrast, E. american B791 and p. cibarius JG-220 did not show any significant activity of histamine degradation. The strains isolated from this study showed relatively fast growth rate and histamine degrading rate as compared to those from salted mackerel.

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피인용 문헌

  1. Predictive modeling of Staphylococcus aureus growth on Gwamegi (semidry Pacific saury) as a function of temperature vol.56, pp.6, 2013, https://doi.org/10.1007/s13765-013-3122-9
  2. Sanitary and Nutritional Characterization of Commercial Kwamegi from Pacific Herring Clupea pallasii vol.45, pp.1, 2012, https://doi.org/10.5657/KFAS.2012.0001
  3. Microbial Decontamination of Gwamegi (Semi-dried Pacific Saury) Using Corona Discharge Plasma Jet, Including Physicochemical and Sensory Evaluation 2018, https://doi.org/10.1080/10498850.2017.1347592
  4. Control of histamine-forming bacteria by probiotic lactic acid bacteria isolated from fish intestine vol.52, pp.3, 2016, https://doi.org/10.7845/kjm.2016.6041
  5. Degradation of histamine by Bacillus polymyxa isolated from salted fish products vol.23, pp.4, 2015, https://doi.org/10.1016/j.jfda.2015.02.003
  6. The Quality Characteristics of Commercial Gwamegi by Product Types vol.16, pp.3, 2011, https://doi.org/10.3746/jfn.2011.16.3.253
  7. Biogenic Amine Content and Hygienic Quality Characterization of Commercial Kwamegi vol.42, pp.5, 2009, https://doi.org/10.5657/kfas.2009.42.5.403