생명과학회지 (Journal of Life Science)

  • 제16권1호
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  • Pages.108-113
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  • 2006
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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Lumbricus rubellus를 이용한 endosulfan의 간편, 신속 독성 평가 및 endosulfan 분해 미생물의 선별

Simple and Rapid Evaluation System for Endosulfan Toxicity and Selection of Endosulfan Detoxifying Microorganism Based on Lumbricus rubellus

  • 손호용 (안동대학교 식품영양학과) ;
  • 김홍주 (안동대학교 식품영양학과) ;
  • 금은주 (안동대학교 식품영양학과) ;
  • 이중복 (안동대학교 생명자원과학부) ;
  • 권기석 (안동대학교 생명자원과학부)
  • Sohn Ho-Yong (Dept. of Food and Nutrition, Andong National University) ;
  • Kim Hong-Ju (Dept. of Food and Nutrition, Andong National University) ;
  • Kum Eun-Joo (Dept. of Food and Nutrition, Andong National University) ;
  • Lee Jung-Bok (The School of Bioresource Sciences, Andong National University) ;
  • Kwon Gi-Seok (The School of Bioresource Sciences, Andong National University)
  • 발행 : 2006.02.01
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초록

독성물질의 무독화 연구의 화학적 분석방법의 문제점을 보완하고, 난분해성 독성물질의 분해 미생물을 효율적으로 탐색하기 위해, 지렁이와 microplate를 이용한 빠르고 간편한 독성평가 시스템을 구축하였다. 본 방법은 지렁이의 사육이나 특수시설이 필요하지 않으며, 치사 및 무게감소가 현저하게 나타나지 않는 저농도, 단기간 처리시에도 신속하게 독성의 정량평가가 가능하다 실제 endosulfan 및 다양한 endosulfan 유도체, 구조적 유사체 등을 대상으로 독성 평가한 결과, endosulfan, endosulfan sulfate, aldrin 및 dieldrin 에서만 독성이 나타나 기존의 연구결과와 잘 일치하였다. 이를 이용하여 endosulfan분해 및 무독화 균주 선별을 시도한 결과, 기존 endosulfan분해 및 무독화 균주로 선별된 KE-1, KE-8, KS-2P균주 처리의 경우에는 지렁이의 독성이 거의 나타나지 않았으나, E. coli, B. subtilis, 및 YSU 균주들을 처리한 경우에는, 지렁이 사멸과 함께 심각한 독성이 나타났다. 따라서, 본 시스템은 독성물질의 독성평가뿐 아니라, 독성물질의 분해 및 무독화 미생물 선별에 매우 유용함을 확인하였다.

To compensate the problems of chemical assay in detoxification of recalcitrant and a practical approach in selection of bioremediation bacteria, a simple and rapid toxicity evaluation system was constructed based on Lumbricus rubellus. Long term-culture and specific equipment are not necessary, and semi-quantitative analysis of toxicity at sub-lethal concentration is possible by measuring of dose-dependent increased yellowish secreted compounds. When the toxicity of endosulfan, its metabolites and structurally related chemicals were measured for 24 h, the results were coincided with previous reports. Toxicity was found in endosulfan, endosulfan sulfate, aldrin, and dieldrin, respectively. Rapid and economic selection of endosulfan-detoxifying bacteria was possible using our system. Klebsiella pneumoniae KE-1, K. oxytoca KE-8 and Pseudomonas sp. KS-2P, reported endosulfan degrading bacteria, ameliorated the endosulfan toxicity, whereas E. coli, B. subtilis and other bacteria failed to protect the toxicity of endosulfan in L. rubellus. Our results suggest that the constructed system is useful to selection of microorganism as well as toxicity evaluation against toxic recalcitrants.

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