Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Genetic Diversity and Population Structure of Pyrola fauriena (Pyrolaceae) in Korea

한국내 주걱노루발의 유전적 다양성과 집단구조

  • Huh, Man-Kyu (Department of Molecular Biology, Dong-eui University)
  • 허만규 (동의대학교 분자생물학과)
  • Published : 2006.02.01
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Abstract

Starch gel electrophoresis was used to estimate genetic diversity and population structure of Pyrola fauriena H. Andr. in Korea. The percentage of polymorphic loci within enzymes was $57.1\%$. The values of genetic diversity at the species level and at the population were higher than average values for herbaceous with similar life history traits (Hes : 0.149; Hep = 0.134, respectively), whereas the extent of the population divergence was relatively low $(G_{ST}=0.082)$. $F_{IS}$, a measure of the deviation from random mating within the 12 populations, was 0.298. An indirect estimate of the number of migrants per, generation (Nm = 2.81) indicates that gene flow is moderate among Korean populations of the species. Analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and at some loci. This indicates that some populations sampled may have been substructured largely due to rhizotamous spread and decrease of population sizes.

한국내 주걱노루발의 유전적 다양성과 집단구조를 평가하기 위해 전분 젤 전기영동을 사용하였다. 효소내 다형현상을 나타내는 대립유전자좌위는 $57.1\%$였다. 종 수준(Hes = 0.149)과 집단 수준(Hep = 0.134)에서 유전적 다양도는 유사한 생활사를 가진 초본류의 평균값에 비해 높았다. 반면에 집단간 분화정도는 비교적 낮았다 $(G_{ST}=0.082)$. 열 두 집단내 임의교배에서 편차는 0.298이었다. 간접적으로 평가한 세대당 이주하는 개체수(Nm = 2.81)는 이 종의 한국 집단내 보통 수준을 유지하고 있었다. 고정지수 분석 결판 많은 집단과 대립유전자좌위에서 이형접합체의 결핍이 있었다. 이는 주걱노루발에서 가근의 번식능력과 한국내 조사된 주걱노루발 집단의 크기 감소에 기인한다.

Keywords

References

  1. Barton, N. H. and M. Slatkin. 1986. Aqusi-equilibrium theory of the distribution of rare alleles in a subpopulation. Heredity 56, 409-415 https://doi.org/10.1038/hdy.1986.63
  2. Brown, A. H. D. 1979. Enzyme polymorphism in plant populations. Theor. Biol. 15, 1-42 https://doi.org/10.1016/0040-5809(79)90025-X
  3. Cook, R. E. 1983. Clonal plant populations. Am. Sci. 71, 244-253
  4. Crawford, D. J. 1983. Phygenetic and Systematic Inferences from Electrophoretic studies, pp. 257-287, In S. D. Thanksley and T. J. Orton (eds.), Isozymes in Plant Breeding, Part A. Elsevier, Amsterdam
  5. Ellstrand, N. C. and M. L. Roose. 1987. Patterns of genotypic diversity in clonal plant species. Am. J. Bot. 74, 123-131 https://doi.org/10.2307/2444338
  6. Fryxell, P. A. 1957. Mode of reproduction in higher plants. Bot. Rev. 23, 135-233 https://doi.org/10.1007/BF02869758
  7. Godt, M. J. W. and J. L. Hamrick. 1991. Genetic variation in Lathyrus latifolius (Leguminosae). Am. J. Bot. 78, 1163-1171 https://doi.org/10.2307/2444920
  8. Gottlieb, L. D. 1981. Electrophoretic evidence and plant populations. Progr. Phytochem. 7, 1-46
  9. Hamrick, J. L., Y. B. Linhart and J. B. Mitton. 1979. Relationships between life history characteristics and electrophoretically detectable genetic variation in plants. Ann. Rev. Ecol. 10, 175-200
  10. Hamrick, J. L. and M. J. W. Godt. 1989. Allozyme Diversity in Plant Species, pp. 43-63, In A. H. D. Brown, M. T. Clegg, A. L. Kahler and B. S. Weir (eds.), Plant Population Genetics, Breeding and Genetic Resources. Sinauer, Sunderland, MA
  11. Hamrick, J. L., M. J. W. Godt and S. L. Sherman-Broyles. 1992. Factors influencing levels of genetic diversity in woody plant species. New Forests 6, 95-124 https://doi.org/10.1007/BF00120641
  12. Hartnett, D. C. and F. A. Bazzaz. 1985. The regulation of leaf, ramet and gene densities in experimental populations of the rizomatous perennial Solidago canadensis. J. of Ecology 73, 429-443 https://doi.org/10.2307/2260485
  13. Ledig, F. T. 1986. Heterozygosity, Heterosis, and Fitness in Outbreeding Plants, pp. 77-104, In M. E. Soule (ed.), Conservation Biology. Sinauer, Sunderland, MA
  14. Li, C. C. and D. G. Horvitz. 1953. Some methods of estimating the inbreeding coefficient. Am. J. Human Genet. 5, 107-117
  15. Murawski, D. A. and J. L. Hamrick. 1990. Local genetic and clonal structure in the tropical terrestrial bromelid, Aechmea magdalenae. Am. J. Bot. 77, 1201-1208 https://doi.org/10.2307/2444631
  16. Nei, M. 1972. Genetic distance between populations. Am. Nat. 106, 282-292
  17. Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. 70, 3321-3323 https://doi.org/10.1073/pnas.70.12.3321
  18. Nei, M., T. Maruyama and R. Chakraborty. 1975. The bottleneck effect and genetic variability in populations. Evolution 29, 1-10 https://doi.org/10.2307/2407137
  19. Nei, M. 1977. F-statistics and analysis of gene diversity in subdivided populations. Ann. Human. Genet. 41, 225-233 https://doi.org/10.1111/j.1469-1809.1977.tb01918.x
  20. SAS Institute, Inc. 1989. SAS/STAT User's Guide, Ver. 6, 4th eds., Vol 1. SAS Institute, Cary, 943 pp
  21. Slatkin, M. 1985. Rare alleles as indicators of gene flow. Evolution 39, 53-65 https://doi.org/10.2307/2408516
  22. Sobey, D. G. and P. Barkhouse. 1977. The structure and rate growth of the rhizome of some forest herbs and dwarf herbs of the New Brunswick-Nova Scotia border region. Can. Field-Naturalist 91, 377-383
  23. Soltis, D. E., C. H. Haufler, D. C. Darrow and G. J. Gastony. 1983. Starch gel electrophoresis of ferns: A compilation of grinding buffers, gel and electrode buffers, and staining schedules. Am. Fern J. 73, 9-27 https://doi.org/10.2307/1546611
  24. Weeden, N. F. and J. F. Wendel. 1989. Genetics of Plant Isozymes, pp. 46-72, In D. E. Soltis and P. S. Soltis (eds.), Isozymes in Plant Biology. Dioscorides Press, Portland, OR
  25. Wolf, P. G. and D. R. Compbell. 1995. Hierarchical analysis of allozymic and morphometric variation in a montane herb, Ipomopsis aggregate (Polemoniaceae). J. Heredity 86, 286-394
  26. Woodland, D. W. 1991. Contemporary Plant Systematics. pp. 222, Prentice-Hall, Inc., Englewood Cliffs, NJ
  27. Workman, P. L. and J. D. Niswander. 1970. Population studies on southern Indian tribes. II. Local genetic differentiation in the Papago. Am. J. Hum. Genet. 22, 24-49
  28. Wright, S. 1965. The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19, 395-420 https://doi.org/10.2307/2406450