Journal of Microbiology

  • 제44권1호
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  • Pages.50-53
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  • 2006
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Identification of Essential Amino acid Residues in Valine Dehydrogenase from Streptomyces albus

  • Hyun Chang-Gu (Division of Bioscience & Bioinformatics, Myongji University) ;
  • Kim Sang-Suk (Division of Bioscience & Bioinformatics, Myongji University) ;
  • Suh Joo-Won (Division of Bioscience & Bioinformatics, Myongji University)
  • 발행 : 2006.02.01
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초록

Cys-29 and Cys-251 of Streptomyces albus valine dehydrogenase(ValDH) were highly conserved in the corresponding region of $NAD(P)^+$-dependent amino acid dehydroganase sequences. To ascertain the functional role of these cysteine residues in S. albus ValDH, site-directed mutagenesis was performed to change each of the two residues to serine. Kinetic analyses of the enzymes mutated at Cys-29 and Cys-251 revealed that these residues are involved in catalysis. We also constructed mutant ValDH by substituting valine for leucine at 305 by site-directed mutagenesis. This residue was chosen, because it has been proposed to be important for substrate discrimination by phenylalanine dehydrogenase (PheDH) and leucine dehydrogenase (LeuDH). Kinetic analysis of the V305L mutant enzyme revealed that it is involved in the substrate binding site. However it displayed less activity than the wild type enzyme toward all aliphatic and aromatic amino acids tested.

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참고문헌

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