한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제35권2호
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  • Pages.177-181
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  • 2006
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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야생 및 재배 지치뿌리의 Shikonin 화합물 확인

Identification of Shikonin and Its Derivatives from Lithospermum erythrorhizon

  • 김진숙 (농업과학기술원 농촌자원개발연구소) ;
  • 한영실 (숙명여자대학교 식품영양학과) ;
  • 강명화 (호서대학교 식품영양학과)
  • Kim, Jin-Sook (National Rural Resources Development Institute, RDA) ;
  • Han, Young-Sil (Dept. of Food and Nutrition, Sookmyung Women's University) ;
  • Kang, Myung-Hwa (Dept. of Food Science and Nutrition, Hoseo University)
  • 발행 : 2006.02.01
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초록

야생 및 재배지치의 methanol 추출물로 조제한 n-herane 용매 분획물에 대하여 shikonin 및 그 유도체 화합물을 표품으로 해서 HPLC/MS로 물질을 분리하였다. 분리된 물질의 구조 확인을 위해 $^1H-NMR$, $^{13}C-NMR$을 통해 분석한 결과, compound I은 화학식이 $C_{16}H_{16}O_5$이며 분자량이 288.3인 shikonin이었고, compound II는 화학식이 $C_{16}H_{16}O_4$이며 분자량이 272.3인 deoxyshikonin것으로 밝혀내었다. 야생지치에 함유된 shikonin과 deoxyshikonin은 $2.23\%,\;0.87\%$이었고, 재배지치에는 각각 $2.24\%,\;0.41\%$이었다. 지치의 동정된 색소물질 총량은 야생지치 $3.12\%$, 재배지치 $2.64\%$로 야생지치가 재배지치보다 유의적으로 많았다(p<0.05).

In this study, phytochemicals from the wold and cultivated Lithospermum erythrorhizon (gromwell), which has been used for medicinal purpose or natural coloring material from the old days, were extracted by methanol and fractionated with hexane. The shikonin compounds in the fraction was isolated and their chemical structures were identified by $^1H$ and $^{13}C-NMR$. It was found that compound I was the shikonin substance with molecular weight of 288.3 and chemical formula of $C_{16}H_{16}O_5$, and compound II being deoxyshikonin substance with molecular weight of 272.3 and chemical formula of $C_{16}H_{16}O_4$. The Quantities of these compounds in the wild and cultivated gromwells was determined.

키워드

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피인용 문헌

  1. Enhancement of Whitening Effects of Lithospermum erythrorhizon Extracts by Ultra High Pressure vol.19, pp.2, 2011, https://doi.org/10.7783/KJMCS.2011.19.2.097
  2. Antioxidant Activity of Solvent Fractions from Cultivated and Wild Gromwell vol.39, pp.6, 2010, https://doi.org/10.3746/jkfn.2010.39.6.789
  3. Improved cosmetic activity by optimizing the Lithospermum erythrorhizon extraction process vol.67, pp.1, 2015, https://doi.org/10.1007/s10616-013-9657-0
  4. Studies on Seed Germination Characteristics and Patterns of Protein Expression of Lithospermum erythrorhizon by Plant Growth Regulators and Seed Primings vol.22, pp.6, 2014, https://doi.org/10.7783/KJMCS.2014.22.6.435
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  6. 황기, 지치 복합물의 연골세포에서의 Matrix Metalloproteinases 저해 효과 및 유효성분의 분석 vol.27, pp.4, 2006, https://doi.org/10.7783/kjmcs.2019.27.4.247