Environmental Mutagens and Carcinogens (한국환경성돌연변이발암원학회지)

Korean Environmental Mutagen Society (한국환경성돌연변이발암원학회)
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Cytotoxcity Effects of Triphlorethol-A on Various Cancer Cells

  • Kang Kyoung-Ah (Department of Biochemistry, College of Medicine, Cheju National University) ;
  • Zhang Rui (Department of Biochemistry, College of Medicine, Cheju National University) ;
  • Piao Mei Jing (Department of Biochemistry, College of Medicine, Cheju National University) ;
  • Lee Nam-Ho (Department of Chemistry, College of Natural Sciences, Cheju National University) ;
  • Park So-Yoon (Department of Pathology, College of Medicine, Cheju National University) ;
  • Hyun Jin-Won (Department of Biochemistry, College of Medicine, Cheju National University)
  • Published : 2006.12.31
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Abstract

Ecklonia cava is a brown alga(Laminariaceae) that is abundant in the subtidal regions of Jeju island in Korea. Phlorotannins were identified to be responsible for the biological activities in Ecklonia species. In the present study, triphlorethol-A, a phlorotannin, was isolated from Ecklonia cava and its anticancer properties were investigated. Triphlorethol-A was investigated whether it may show cytotoxicity effects against U937, HeLa, NCI-H460 and MCF-7 cancer cells by MTT test. As a result, triphlorethol-A did not show cytotoxic effects against tested four cell lines.

Keywords

References

  1. Ahn, M.J., Yoon, K.D., Min, S.Y., Lee, J.S., Kim, J.H., Kim, T.G., Kim, S.H., Kim, N.G., Huh, H. and Kim, J. (2004): Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava. Biol. Pharm. Bull., 27, 544-547 https://doi.org/10.1248/bpb.27.544
  2. Borek, C. (1991): Free radical processes in multistage carcinogenesis. Free Radic. Res. Comm., 12, 745-750
  3. Borek, C. (1997): Antioxidants and cancer. Sci. Med. (Phila), 4, 51-62
  4. Borek, C., Ong, A., Mason, H., Donahue, L. and Biaglow, J.E. (1986): Selenium and vitamin E inhibit radiogenic and chemically induced transformation in vitro via different mechanisms. Proc. Natl. Acad. Sci. USA, 83, 1490-1494
  5. Carmichael, J., DeGraff, W.G., Gazdar, A.F., Minna, J.D. and Mitchell, J.B. (1987): Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer. Res., 47, 936-941
  6. Fukuyama, Y., Kodama, M., Miura, I., Kinzyo, Z., Kido, M., Mori, H., Nakayama, Y. and Takahashi, M. (1989a): Structure of an anti-plasmin inhibitor, eckol, isolated from the brown alga Ecklonia kurome Okamura and inhibitory activities of its derivatives on plasma plasmin inhibitors. Chem. Pharm. Bull., 37, 349-353 https://doi.org/10.1248/cpb.37.349
  7. Fukuyama, Y., Kodama, M., Miura, I., Kinzyo, Z., Mori, H., Nakayama, Y. and Takahashi, M. (1990): Anti-plasmin inhibitor. VI. Structure of phlorofucofuroeckol A, a novel phlorotannin with both dibenzo-1,4-dioxin and dibenzofuran elements, from Ecklonia kurome Okamura. Chem. Pharm. Bull. (Tokyo), 38, 133-135 https://doi.org/10.1248/cpb.38.133
  8. Fukuyama, Y., Kodama, M., Miura, I., Kinzyo, Z., Kido, M., Mori, H., Nakayama, Y. and Takahashi, M. (1989b): Structure of an anti-plasmin inhibitor, eckol, isolated from the brown alga Ecklonia kurome Okamura and inhibitory activities of its derivatives on plasma plasmin inhibitors. Chem. Pharm. Bull. (Tokyo), 37, 349-353 https://doi.org/10.1248/cpb.37.349
  9. Fukuyama, Y., Kodama, M., Miura, I., Kinzyo, Z., Mori, H., Nakayama, Y. and Takahashi, M. (1989): Anti-plasmin inhibitor. V. Structures of novel dimeric eckols isolated from the brown alga Ecklonia kurome Okamura. Chem. Pharm. Bull. (Tokyo), 37, 2438-2440 https://doi.org/10.1248/cpb.37.2438
  10. Gutteridge, J.M.C. (1993): Free radicals in disease processes: a compilation of cause and consequence. Free Radic. Res.Commun., 19, 141-158 https://doi.org/10.3109/10715769309111598
  11. Kang, K., Park, Y., Hwang, H.J., Kim, S.H., Lee, J.G. and Shin, H.C. (2003): Antioxidative properties of brown algae polyphenolics and their perspectives as chemopreventive agents against vascular risk factors. Arch. Pharm. Res., 26, 286-293 https://doi.org/10.1007/BF02976957
  12. Kang, H.S., Chung, H.Y., Kim, J.Y., Son, B.W., Jung, H.A. and Choi, J.S. (2004): Inhibitory phlorotannins from the edible brown alga Ecklonia stolonifera on total reactive oxygen species (ROS) generation. Arch. Pharm. Res., 27, 194-198 https://doi.org/10.1007/BF02980106
  13. Kim, J.A., Lee, J.M., Shin, D.B. and Lee, N.H. (2004): The antioxidant activity and tyrosinase inhibitory activity of phlorotannins in Ecklonia cava. Food Sci. Biotechnol., 13, 476-480
  14. Nakamura, T., Nagayama, K., Uchida, K. and Tanaka, R. (1996): Antioxidant activity of phlorotannins isolated from he brown alga Eisenia bicyclis. Fish Sci., 62, 923-926 https://doi.org/10.2331/fishsci.62.923
  15. Park, D.C., Ji, C.I., Kim, S.H., Jung, K.J., Lee, T.G., Kim, I.S., Park, Y.H. and Kim, S.B. (2000): Characteristics of tyrosinase inhibitory extract from Ecklonia stolonifera. J.Fish Sci. Tech., 3, 195-199
  16. Shibata, T., Yamaguchi, K., Nagamura, K., Kawaguchi, S. and Nagamura, T. (2002): Inhibitory activity of brown algae phlorotannins against glycosidases from the viscera of the turban shell Turbo cornutus. Eur. J. Phycol., 37, 493-500 https://doi.org/10.1017/S0967026202003918
  17. Shi, X., Castranova, V., Halliwell, B. and Vallyathan, V. (1998): Reactive oxygen species and silica induced carcinogenesis. J. Toxicol. Environ. Health Part B, Crit. Rev., 1, 181- 197 https://doi.org/10.1080/10937409809524551
  18. Sporn, M.B. and Suh, N. (2000): Chemoprevention of cancer. Carcinogenesis, 21, 525-530 https://doi.org/10.1093/carcin/21.3.525