Archives of Plastic Surgery

  • 제33권5호
  • /
  • Pages.612-615
  • /
  • 2006
  • /
  • 2234-6163(pISSN)
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  • 2234-6171(eISSN)
대한성형외과학회 (Korean Society of Plastic and Reconstructive Surgeons)
권호 표지

우태아 혈청이 포함된 Poly-glycolic Acid 배양판에서 인간 조골세포의 성장

The Growth of Human Osteoblasts in Culture Dishes Made with Poly-glycolic Acid Containing Fetal Bovine Serum

  • 최재원 (영남대학교 의과대학 성형외과학교실) ;
  • 김용하 (영남대학교 의과대학 성형외과학교실) ;
  • 문영미 (영남대학교 의과대학 의료공학 연구소) ;
  • 김연정 (안동대학교 자연과학대학 응용화학과) ;
  • 최식영 (안동대학교 자연과학대학 응용화학과)
  • Choi, Jae Won (Department of Plastic & Reconstructive Surgery, College of Medicine, Yeungnam University) ;
  • Kim, Yong Ha (Department of Plastic & Reconstructive Surgery, College of Medicine, Yeungnam University) ;
  • Moon, Young Mi (Department of Tissue Engineering, College of Medicine, Yeungnam University) ;
  • Kim, Yoen Jung (Applied Chemistry, Natural Science, Andong National University) ;
  • Choi, Sik Young (Applied Chemistry, Natural Science, Andong National University)
  • 투고 : 2006.04.27
  • 발행 : 2006.09.10
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초록

Purpose: An ideal bony construct can be divided into two broad categories: (1) the design and fabrication of biodegradable, biomimetic scaffolds that provide correct signals to induce osteogenesis: (2) the identification of an ideal source of osteoprogenitor cells to seed onto the scaffold. We selected poly-glycolic acid as a synthetic scaffold among various scaffolds because of these properties. Meanwhile, culture medium is supplemented with fetal bovine serum(FBS): such serum contains essential elements such as proteins, hormones, growth factors and trace minerals. The composition of FBS can be ideal for various cell growth in vitro. We supposed that we could enhance bone growth at a fractured site if FBS was mixed with synthetic scaffold-PGA. Methods: We cultured human osteoblasts in five different prepared culture dishes made with FBS and PGA mixture. The mixtures contained different ratio of FBS, that is, 0, 1.5, 3, 7, and 10%. We cultured human osteoblasts for seven days and examined the growth and attachment of the cells at the 1st, 3rd, 5th, 7th days, respectively. Results: In the mixture of 0% FBS and PGA, the growth of the cells lasted for one day. In 1.5 and 3% FBS and PGA, the growth of the cells was examined at the 3rd day, then minimally declined at the 5th and 7th days. In 7% FBS and PGA, the growth of the cells lasted for 5 days, then declined at the 7th day. In 10% FBS and PGA, the growth of the cells lasted for 5 days, then declined at the 7th day. Staining status of the osteoblasts with alkaline phosphatase showed pale pink color in 0% FBS and PGA groups, but bright pink color in 1.5, 3, 7, 10% FBS and PGA groups, especially in 3%, 7%. Conclusion: In consequence, the growth of human osteoblast was higher in the mixture of FBS and PGA groups than in pure PGA ones. It is assumed that the mixture of FBS and PGA affects the proliferation of human osteoblasts.

키워드

과제정보

연구 과제 주관 기관 : 재단법인 천마의학연구재단

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