Comparative Studies on Detecting Methods of Fecal Indicators (Coliforms) in Surface Water

지표수에서의 분변오염지표세균 (대장균군) 검출방법의 비교연구

  • Park, Ji-Eun (Department of Life Science, Daegu University) ;
  • Kim, Sun-Duck (Department of Chemistry/App. Chemistry, Daegu University) ;
  • Cho, Ju-Rae (Center of Int. Drinking Water, Korea Water Resources Corperation) ;
  • Kim, Sang-Hyun (Department of Environmental Engineering, Pusan National University) ;
  • Lee, Hae-Jin (Nakdong River Water Environment Research, National Institute of Environmental Research) ;
  • Lee, Young-Ok (Department of Life Science, Daegu University)
  • 박지은 (대구대학교 생명과학과) ;
  • 김선덕 (대구대학교 화학.응용화학과) ;
  • 조주래 (한국수자원공사 국제수돗물센터) ;
  • 김상현 (부산대학교 환경공학과) ;
  • 이혜진 (국립환경과학원 낙동강물환경연구소) ;
  • 이영옥 (대구대학교 생명과학과)
  • Received : 2006.08.14
  • Accepted : 2006.09.21
  • Published : 2006.11.30

Abstract

For monitoring the fecal pollution at Nak-Dong River, one of the eutrophicated rivers, the differences between total coliforms (TC) and fecal coliforms (FC) using both of membrane filtration (MF)/MPN method, and also fecal streptococcus (FS) by MF-method was investigated. To evaluate the correlation between TC, FC, and FS statistical analyses were performed by using Minitab. And a part of the presumptive TC/FC and background colonies was purified and identified using API 20E kit (Biomeriux). As results, most (89%) of presumptive FC by MF was identified as Escherichia coli while only 14% (MPN) and 11% (MF) of TC were identified as E. coli. Furthermore, FC by MF was correlated significantly with other fecal indicators (TC/FS by MF and FC by MPN), while TC by MPN was not correlated with any other indicators. Thus, the detection of FC by MF-method may be the most reasonable for monitoring the fecal pollution.

Keywords

Acknowledgement

Supported by : 낙동강 물환경 연구소

References

  1. 박지은, 이영옥, 총대장균군 측정시 나타나는 위양성 및 위 음성의 문제점과 해결방안, 제 13 희 물의 날 기념 심포지움, 강원지역환경기술개발센터 주최, pp. 27-37 (2005)
  2. 이목영, 조은주, 최선영, 총대장균군 및 분원성 대장균군 측정방법 비교, 제 13 회 물의 날 기념 심포지움, 강원지역 환경기술개발센터 주최, pp. 41-48 (2005)
  3. 이영옥, 지표수에서 대장균검출에 관한 비교연구, 한국육수학회지, 29, pp. 313-321 (1996)
  4. 장현정, 이용욱, 상수원 수질관리를 위한 분변오염 지표세균에 관한 연구, 한국환경위생학회지, 29(1), pp. 19-27 (2003)
  5. 정현미, 박상정, 정원화, 권오상, 박준수, 오덕화, 오상헌, 대장균군의 하천 및 호소 수질 환경기준 적용성에 관한 연구, 국립환경연구원보, 23, pp. 315-328 (2001)
  6. 환경부, 수질오염공정시험방법, 환경부고시 제 2004-188호 (2004)
  7. Alonso, J. L., Amoros, I. and Alonso, M., Differential Susceptibility of Aeromonads and Coliforms to Cefsulodin, Appl. Environ. Microbiol., 62, pp. 1885-1888 (1996)
  8. APHA-AWWA-WPCF, Standard Methods for the Examination of Water and Wastewater. 19th ed. APHA. N. Y. (1995)
  9. Brenner, K. P., Rankin, C. C., Roybal, Y. R., Stelma, G. N., Scarpino, P. V. and Dupour, A. P., New Medium for the Simultaneous Detection of Total Colifonns and Escherichia coli in Water, Appl. Environ. Microbiol., 59, pp. 3534-3544 (1993)
  10. Burlingame, G. A., McElhaney, J., Bennett, M. and Pipes, W. O., Bacterial Interference with Coliform Colony Sheen Production on Membrane Filters, Appl. Environ. Microbiol., 47, pp. 56-60 (1987)
  11. Duncan, D. W. and Razzell, W. E., Klebsiella Biotypes among Coliforms Isolated from Forest Environments and Farm Produce, Appl. Microbiol., 24, pp. 933-938 (1972)
  12. Edberg, S. C., Rice, E .W., Karlin, R. J. and Allen, M. J., Escherichia coli: the Best Biological Drinking Water Indicator for Public Health Protection, J. Appl. Microbiol., 88, pp. 106-116 (2000) https://doi.org/10.1111/j.1365-2672.2000.tb05338.x
  13. Hazen, T. C., Fliermans, C. B., Hirsch, R. P. and Esch, G. W., Prevalence and Distribution of Aeromonas hydrophila in the United States, Appl. Environ. Microbiol., 36, pp. 731-738 (1978)
  14. ISO 7899-2, International standard, Water quality - Detection and Enumeration of fecal streptococci - part 2: method by membrane filtration 1. ed. ISO (the International Organization for Standardization), Switzerland (2001)
  15. ISO 9308, International standard, Water quality - Detection and enumeration of coliform organisms, thermotolerant coliform organisms and presumptive Escherichia coli, I. ed. ISO, Switzerland (1990)
  16. Lavoie, M. C., Identification of Strains Isolated as Total and Fecal Coliforms and Comparison of Both Groups as Indicatiors of Fecal Pollution in Tropical Climates, Can. J. Microbiol, 29, pp. 689-693 (1983) https://doi.org/10.1139/m83-112
  17. Oberzill, W., Mikrobiologische Analytik. Verlag Hans Carl, Nurnberg, pp. 519 (1967)
  18. Rippey, S. R. and Cabelli, V. J., Occurrence of Aeromonas hydrophila in Limnetic Environments: Relationship of the Organism to Trophic State, Microb. Ecol., 6, pp. 45-54 (1980) https://doi.org/10.1007/BF02020374