Archives of Plastic Surgery

  • 제33권4호
  • /
  • Pages.427-432
  • /
  • 2006
  • /
  • 2234-6163(pISSN)
  • /
  • 2234-6171(eISSN)
대한성형외과학회 (Korean Society of Plastic and Reconstructive Surgeons)
권호 표지

헤파린이 수근관 증후 군 환자의 횡수근 인대에서 추출한 섬유아세포의 콜라겐, MMP 및 TGF-β 동위형 발현에 미치는 영향

The Effect of Heparin on Gene Expression of Collagen, MMP, TGF-β Isoforms Using Cultured Fibroblast from Transverse Carpal Ligament of Carpal Tunnel Syndrome

  • 정성호 (고려대학교 의과대학 성형외과학교실) ;
  • 나민화 (이화여자대학교 의과대학 성형외과학교실) ;
  • 이재선 (고려대학교 의과대학 성형외과학교실) ;
  • 송상훈 (고려대학교 의과대학 성형외과학교실) ;
  • 윤을식 (고려대학교 의과대학 성형외과학교실) ;
  • 동은상 (고려대학교 의과대학 성형외과학교실) ;
  • 김한중 (이화여자대학교 의과대학 성형외과학교실)
  • Jeong, Seong Ho (Department of Plastic and Renconstructive Surgery, Korea University College of Medicine) ;
  • Na, Min Hwa (Department of Plastic and Reconstructive Surgery, Ehwa Wemen's University College of Medicine) ;
  • Lee, Jae Sun (Department of Plastic and Renconstructive Surgery, Korea University College of Medicine) ;
  • Song, Sang Hun (Department of Plastic and Renconstructive Surgery, Korea University College of Medicine) ;
  • Yoon, Eul Sik (Department of Plastic and Renconstructive Surgery, Korea University College of Medicine) ;
  • Dhong, Eun Sang (Department of Plastic and Renconstructive Surgery, Korea University College of Medicine) ;
  • Kim, Han Joong (Department of Plastic and Reconstructive Surgery, Ehwa Wemen's University College of Medicine)
  • 투고 : 2006.03.08
  • 발행 : 2006.07.10
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초록

Purpose: Noninflammatory synovial fibrosis has been noted for main causal factor of carpal tunnel syndrome (CTS). Recently, there are some reports that heparin have not only anti-coagulative effect but also anti-inflammatory and anti-fibrotic potential and have an effect on interstitial pulmonary fiborosis. Authors examined whether heparin affects pathogenesis of CTS. Methods: First, heparin was administered to fibroblast that was cultured from patient's transverse carpal ligament. Secondly, we evaluated the expression from genes of type I, III collagen, TGF ${\beta}$ isoforms and MMP. Fibroblasts were isolated and cultured from transverse carpal ligaments of 5 patients with CTS. Heparin (0, 1, 10,$100{\mu}g/ml$) was administered to cultured fibroblast and reverse transcription PCR for mRNA expression of type I, III collagen, TGF-${\beta}$ isoforms and MMP was done. Results: Heparin suppressed gene expression of type I, III collagen and TGF-${\beta}1$, ${\beta}3$ but promoted gene expression of TGF-${\beta}2$ and MMP-2. Conclusion: Heparin directly suppress gene expression of type I, III collagen. But, It is undetermined that heparin can present it's effect mediated by TGF ${\beta}$ isoforms or MMP.

키워드

참고문헌

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