한국산림과학회지 (Journal of Korean Society of Forest Science)

  • 제95권2호
  • /
  • Pages.209-215
  • /
  • 2006
  • /
  • 2586-6613(pISSN)
  • /
  • 2586-6621(eISSN)
한국산림과학회 (Korean Society of Forest Science)
권호 표지

Antioxidant Characteristics in the Leaves of 14 Coniferous Trees under Field Conditions

  • Han, Sim-Hee (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Lee, Jae-Cheon (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Lee, Wi Young (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Park, YoungKi (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Oh, Chang-Young (Department of Forest Genetic Resources, Korea Forest Research Institute)
  • 투고 : 2006.02.14
  • 심사 : 2006.04.05
  • 발행 : 2006.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

We investigated antioxidant capacity in leaves of 14 coniferous trees under field conditions. We focused on understanding the species characteristics on antioxidant systems and screening the coniferous tree species with the best antioxidant systems using their characteristics. The antioxidant capacity of 14 coniferous trees was divided into three groups. First group was Thuja orientalis and Chamaecyparis obtusa and those species had the highest content of ${\beta}$-carotene and xanthophyll. Second group, C. obtusa and Juniperus chinensis, used antioxidant enzymes to mitigate stress. C. obtusa represented high activity at superoxide dismutase (SOD), glutathione reductase (GR), and peroxidase (POD), and J. chinensis exhibited high activity at SOD, POD, catalase (CAT). Third group employed antioxidant such as ascorbic acid and ${\alpha}$-tocopherol. The antioxidant content of T. orientalis was the highest while that of Pinus parviflora and C. obtusa were the lowest. Few species belonged in three groups simultaneously, and most species belonged in at least one or two groups. In summary, we proposed that C. obtusa and T. orientalis had the highest antioxidant capacity while P. parviflora and P. desiflora for. multicalus had the lowest antioxidant capacity.

키워드

참고문헌

  1. Asada, K., 1997. The role of ascorbate peroxidase and monodehydroascorbate reductase in $H_2O_2$ scavenging in plants. pp. 559-566. In: Scandalios, J.G. (Eds.) Oxidative Stress and the Molecular Biology of Antioxidant Defenses. Cold Spring Harbor Laboratory Press, New York
  2. Baisak, R., Rana, D., Acharya, P.B.B. and M. Kar. 1994. Alterations in the activities of active oxygen scavenging enzymes of wheat leaves subjected to water stress. Plant Cell Physiol. 35: 489-495
  3. Beauchamp, C. and Fridovichi, I. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-297 https://doi.org/10.1016/0003-2697(71)90370-8
  4. Brand-Williams, W. 1995. Use of a free radical method to evaluate antioxidant activity. Food Sci. Tech. (London) 28: 25-30
  5. Calatayud, A., and Barreno, E. 2004. Response to ozone in two lettuce varieties on chlorophyll a fluorescence, photosynthetic pigments and lipid peroxidation. Plant Physiology and Biochemistry 42: 549-555 https://doi.org/10.1016/j.plaphy.2004.05.002
  6. Carlberg, I., and Mannervik, B. 1985. Glutathione reductase. Methods in Enzymology 113: 485-490
  7. Dalton, D., Russel, S., Hanus, F., Pascoe, G and Evans, H. 1986. Enzymatic reactions of ascorbate and glutathione that prevent peroxide damage in soy bean root nodules. Proc. Natl. Acad. Sci. U.S.A. 83: 3811-3815
  8. Fossati P., Prencipe, L. and Berti, G. 1980. Use of 3,5dichloro-2-hydroxy benzenesulfonic acid /4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine. The Clinical Chemistry Methodology 26: 227-231
  9. Foyer, C.H., Lelandais, M. and Kunert, K.J. 1994. Photooxidative stress in plants. Physiol. Plant 92: 696-717 https://doi.org/10.1111/j.1399-3054.1994.tb03042.x
  10. Gressel, J. and Galun, E. 1994. Genetic controls of photooxidant tolerance. pp. 237-274. In: Foyer, C.H. and Mullineaux, P.M. (Eds.), Causes of photooxidative stress and amelioration of defense systems in plant. Boca Raton: CRC Press
  11. Hiscox, J.D. and Israelstam, G.F. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Can. J. Bot. 57: 1332-1334 https://doi.org/10.1139/b79-163
  12. Kolb, T.E., Fredericsen, T.S., Steiner, K.C. and Skell,. J.M. 1997. Issues in scaling tree size and age responses to ozone: a review. Environ. Pollut. 98: 195-208 https://doi.org/10.1016/S0269-7491(97)00132-2
  13. Larson, R.A. 1995. Defenses against oxidative stress. Arch. Insect Biochem. 29: 175-186 https://doi.org/10.1002/arch.940290207
  14. Lichtenthaler, H.K. 1987. Chlorophylls and carotenoids : pigments of photosynthetic biomembranes. Methods in Enzymology 148: 350-382 https://doi.org/10.1016/0076-6879(87)48036-1
  15. Minkov l.N., Jahoubjan, GT. Denev, I.D. and Toneva, V.T. 1999: Photooxidative stress in higher plants. pp. 499-525. In: Pessrakli, M. (Eds.), Handbook of Plant and Crop Stress, 2nd edition. Marcel Decker, New York, Basel
  16. Moore, T.C. 1974. Research Experiences in Plant Physiology. Springer, New York
  17. Noctor, G and Foyer, C. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Phys. 49: 249-279 https://doi.org/10.1146/annurev.arplant.49.1.249
  18. Oncel, I., Yurdakulol, E., Kele? Y. Kurtm, L. and Yildiz, A. 2004. Role of antioxidant defence system and biochemical adaptation on stress tolerance of high mountain and steppe plants. Acta Oecologia 26: 211-218 https://doi.org/10.1016/j.actao.2004.04.004
  19. Pasqualini, S., Batini, P., Ederlina, L., Porceddu, A., Picciont, C., De Marchis, F. and Antonielli, M. 2001. Effects of short-term ozone fumigation on tobacco plants: response of the scavenging system and expression of the glutathione reductase. Plant Cell Environ. 24: 245-252 https://doi.org/10.1111/j.1365-3040.2001.00671.x
  20. Polle, A., Rennenberg, H. and Schol, .F. 1999a. Antioxidative systems in spruce clones grown at high altitudes. Phyton (Austria) 39: 155-164
  21. Polle, A., Baumbusch, L.O., Oschinski, C., Eiblmeier, M., Kuhlenkamp, V., Vollrath, B., Scholz, F. and Rennenberg, H. 1999b. Growth and protection against oxidative stress in young clones and mature spruce trees (Picea abies L.) at high altitudes. Oecologia 12: 149-156
  22. PolIe, A., Otter, T. and Seifert, F. 1994. Apoplastic peroxidases and lignification in needles of Norway spruce (Picea abies L.). Plant Physiol. 106: 53-60 https://doi.org/10.1104/pp.106.1.53
  23. Scandalios, J.G. 1993. Oxygen stress and superoxide dismutases. Plant Physiol. 101: 7-12 https://doi.org/10.1104/pp.101.1.7
  24. Schmieden, U. and Wild, A. 1994. Changes in levels of ${\alpha}$-tocopherol and ascorbate in spruce needles at three low mountain sites exposed to $Mg^{2+}$ deficiency and ozone. Z. Naturforsch. C49: 171-180
  25. Schwanz, P., Picon, C., Vivin, P., Dreyer, E., Guehl, J.M. and Polle, A. 1996. Responses of the antioxidative systems to drought stress in pendunculate oak and maritime pine as affected by elevated $CO_2$. Plant Physiol. 100: 393-402
  26. Sgherri, C.L. and Navari-Izzo, F. 1995. Sunflower seedlings subjected to increasing water deficit stress: oxidative stress and defense mechanisms. Physiol. Plant 93: 25-30 https://doi.org/10.1034/j.1399-3054.1995.930105.x
  27. Siefermann-Harms, D. 1987. The light-harvesting and protective functions of carotenoids in photosynthetic membranes. Physiologia Plantarum 69: 561- 568 https://doi.org/10.1111/j.1399-3054.1987.tb09240.x
  28. Smirnoff, N. 1993. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol. 125: 27-58 https://doi.org/10.1111/j.1469-8137.1993.tb03863.x
  29. Streb, P., Telor, E. and Feierabend, J. 1997. Light stress effects and alternative protection in two desert plants. Funct. Ecol. 11: 416-424 https://doi.org/10.1046/j.1365-2435.1997.00105.x
  30. Tanaka, K., Suda, Y. Kondo, N. and Sugahara, K. 1985. $O_3$ tolerance and the ascorbate-dependent $H_2O_2$ decomposing system in chloroplasts. Plant Cell Physiol. 26: 1425-1431
  31. Young, A. and Britton, G. 1990. Carotenoids and stress, in : R.G. Alscher, J.R. Cummings (Eds.) Stress Responses in Plants: Adaptation and Acclimation Mechanisms, Wiley-Liss, NY, pp.87-112