Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Functional Characterization of NtCDPK1 in Tobacco

  • Lee, Sang Sook (Laboratory of Plant Genomics, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoon, Gyeong Mee (Laboratory of Plant Genomics, Korea Research Institute of Bioscience and Biotechnology) ;
  • Rho, Eun Jung (Department of Biological Sciences, Ajou University) ;
  • Moon, Eunpyo (Department of Biological Sciences, Ajou University) ;
  • Pai, Hyun-Sook (Division of Bioscience and Bioinformatics, Myongji University)
  • Received : 2005.09.22
  • Accepted : 2005.10.10
  • Published : 2006.02.28
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Abstract

We previously showed that NtCDPK1, a tobacco calcium-dependent protein kinase, interacts with and phosphorylates the Rpn3 regulatory subunit of the 26S proteasome, and that both NtCDPK1 and Rpn3 are mainly expressed in rapidly proliferating tissues, including shoot and root meristem. In this study, we examined NtCDPK1 expression in roots using GUS expression in transgenic Arabidopsis plants, and investigated its function in root development by generating transgenic tobacco plants carrying a sense NtCDPK1 transgene. GUS activity was first detected in roots two days after sowing. In later stages, strong GUS expression was detected in the root meristem and elongation zone, as well as the initiation sites and branch points of lateral roots. Transgenic tobacco plants in which NtCDPK1 expression was suppressed were smaller, and their root development was abnormal, with reduced lateral root formation and less elongation. These results suggest that NtCDPK1 plays a role in a signaling pathway regulating root development in tobacco.

Keywords

Acknowledgement

Supported by : Ministry of Science and Technology

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