Journal of Dental Rehabilitation and Applied Science (구강회복응용과학지)

Korean Academy of Stomatognathic Function and Occlusion (대한턱관절교합학회)
권호 표지

Effect of Blasted or Anodized Titanium Surface Roughness on Adhesion and Differentiation of Osteoblasts

블라스팅과 양극산화된 티타늄 표면이 조골세포의 부착 및 분화에 미치는 영향

  • Park, Chan-Jin (Department of Prosthodontics, College of Dentistry, Kangnung National University and Oral Science Institute) ;
  • Cho, Lee-Ra (Department of Prosthodontics, College of Dentistry, Kangnung National University and Oral Science Institute) ;
  • Yi, Yang-Jin (Department of Prosthodontics, College of Dentistry, Kangnung National University and Oral Science Institute) ;
  • Ko, Sung-Hee (Department of Pharmacology, College of Dentistry, Kangnung National University and Oral Science Institute)
  • 박찬진 (강릉대학교 치과대학 치과보철학교실 및 구강과학연구소) ;
  • 조리라 (강릉대학교 치과대학 치과보철학교실 및 구강과학연구소) ;
  • 이양진 (강릉대학교 치과대학 치과보철학교실 및 구강과학연구소) ;
  • 고성희 (강릉대학교 치과대학 약리학교실 및 구강과학연구소)
  • Published : 2006.09.30
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Abstract

The success of an implant is determined by its integration into the tissue surrounding the biomaterial. Surface roughness is considered to influence the behavior of adherent cells. The aim of this in vitro study was to determine the effect of surface roughness on Saos-2 osteoblast-like cells. Titanium disks, blasted with $75{\mu}m$ aluminum oxide particles and anodic oxidized and machined titanium disks were prepared. Saos-2 were plated on the disks at a density of 50,000 cells per well in 48-well dishes. After 1 hour, 1 day, 6 days cell numbers were counted. One day, 6 days after plating, alkaline phosphatase(ALPase) activity was determined. Compared to experimental groups, the number of cells was significantly higher on control group. The stimulatory effect of surface roughness on ALPase was more pronounced on the experimental groups than on control group. These results demonstrate that surface roughness alters proliferation and differentiation of osteoblasts. The results also suggest that implant surface roughness may play a role in determining phenotypic expression of cells.

Keywords

Acknowledgement

Supported by : 강릉대학교 치과병원

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