The Study on Decomposition of Metal-working Fluids Against Microbes

미생물을 이용한 절삭유제의 부패성능 평가에 관한 연구

  • Kim, Young-Wun (Surfactant & Lubricant Research Team, New Chemistry Division, Korea Research Institute of Chemical Technology) ;
  • Hong, Kwang Min (Chungbuk Mechatronics High School) ;
  • Chung, Kunwo (Surfactant & Lubricant Research Team, New Chemistry Division, Korea Research Institute of Chemical Technology) ;
  • Park, Chan-Jo (Surfactant & Lubricant Research Team, New Chemistry Division, Korea Research Institute of Chemical Technology)
  • 김영운 (한국화학연구원 신화학연구단 유제화학연구센터) ;
  • 홍광민 (충북전산기계고등학교) ;
  • 정근우 (한국화학연구원 신화학연구단 유제화학연구센터) ;
  • 박찬조 (한국화학연구원 신화학연구단 유제화학연구센터)
  • Received : 2006.05.29
  • Accepted : 2006.06.28
  • Published : 2006.08.31

Abstract

Growth curves of microbes were examined to evaluate decomposition of metal-working fluids and decomposition properties of metal-working fluids were experimented using controled microbes such as E. coli and K. pnemoniae. According to the results of growth curve of microbes, the growth period depended on species of microbes, 2 h of E. coli, 3 h of K. pneumoniae, 4 h of P. aeruginosa and 3 h of P. oleovarans after incubation. The colony count of E. coli and K. pneumoniae controled to OD of 0.5 ranged from $4.4{\sim}10.0{\times}10^5CFU/mL$ and $1.8{\sim}9.5{\times}10^7CFU/mL$, respectively. The decomposition of metal-working fluids was excellently progressed in the range of pH 6~8 than below pH 4 and above pH 10. In the case of controled fluids to pH 6~8, the decomposition of the fluid containing ester group was more accelerated than that of the fluid containing ethylene glycol.

절삭유제의 미생물에 대한 부패능을 평가하기 위하여 미생물의 성장 유형을 조사하고 조절된 균을 절삭유제에 접종하여 부패능을 실험하였다. 미생물의 성장곡선 실험 결과, 균의 대수성장기는 균의 종류에 따라 다르게 나타났으며 E. coli 균의 대수 성장기는 배양 후 2시간, K. pneumoniae 균은 3시간, P. aeruginosa 균은 4시간, P. oleovarans 균은3시간이었다. OD 값을 0.5로 조절한 접종액의 E. coli 균수는 $4.4{\sim}10.0{\times}10^5CFU/mL$, K. pneumoniae 균수는 $1.8{\sim}9.5{\times}10^7CFU/mL$이었다. 미생물의 부패에 대한 pH의 영향을 살펴본 결과, pH 6~8에서 부패가 왕성하게 진행되었고 pH가 4 이하, 10이상에서는 균이 성장하지 못함을 알 수 있었다. pH를 중성으로 유지하고 내마모제의 구조에 따른 부패능을 실험한 결과, 에스테르형 내마모제가 에틸렌글리콜형 내마모제 보다 부패가 빠르게 진행되어 구조에 따라 부패능의 차이를 나타내었다.

Keywords

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