한국물환경학회지 (Journal of Korean Society on Water Environment)

  • 제22권6호
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  • Pages.1014-1021
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  • 2006
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  • 2289-0971(pISSN)
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  • 2289-098X(eISSN)
한국물환경학회 (Korean Society on Water Environment)
권호 표지

완전침지형 회전매체공정 내 질산화 및 탈질 관련 미생물의 군집 분포

Diversity of Nitrifying and Denitrifying Bacteria in SMMIAR Process

  • Quan, Zhe-Xue (Department of Microbiology and Microbial Engineering, Fudan University) ;
  • Lim, Bong-Su (Department of Environmental Engineering, Daejeon University) ;
  • Kang, Ho (Department of Environmental Engineering, Chungnam National University) ;
  • Yoon, Kyung-Yo (Donsung E&G Co. Ltd.) ;
  • Yoon, Yeo-Gyo (Donsung E&G Co. Ltd.)
  • 투고 : 2006.05.11
  • 심사 : 2006.09.21
  • 발행 : 2006.11.30
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초록

SMMIAR (Submerged Moving Media Intermittent Aeration Reactor) Process is a very efficient system which remove ammonia to nitrogen gas in one reactor. In this study, we determined the diversity of ammonia oxidizing bacteria and denitrifying bacteria using specific PCR amplification and the clone library construction. An ammonia monooxygenase gene(amoA) was analyzed to investigate the diversity of nitrifiers. Most of amoA gene fragments (27/29, 93%) were same types and they are very similar (>99%) to the sequences of Nitrosomonas europaea and other clones isolated from anoxic ammonia oxidizing reactors. ANAMMOX related bacteria have not determined by specific PCR amplification. A nitrite reductase gene(nirK) was analyzed to investigate the diversity of denitrifying bacteria. About half (9/20, 45%) of denitrifiers were clustered with Rhodobacter and most of others were clustered with Mesorhizobium (6/20, 30%) and Rhizobium (3/20, 15%). All of these nirK gene clones were clustered in alpha-Proteobacteria and this result is coincide with other system which also operate nitrification and denitrification in one reactor. The molecular monitoring of the population of nitrifiers and denitrifiers would be helpful for the system stabilization and scale-up.

키워드

과제정보

연구 과제 주관 기관 : 대전환경기술개발센터

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