Clinical and Experimental Pediatrics

The Korean Pediatric Society (대한소아청소년과학회)
권호 표지

Molecular detection of Bartonella henselae DNA from fleas obtained from dogs, Korea

개에서 추출된 벼룩에서 분자생물학적 방법에 의한 Bartonella henselae DNA의 검출

  • Han, Tae Hee (Department of Laboratory Medicine, Inje University Sanggye Paik Hospital, Inje University College of Medicine) ;
  • Chung, Ju-Young (Department Pediatrics, Inje University Sanggye Paik Hospital, Inje University College of Medicine) ;
  • Seong, Hee Kyung (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje University) ;
  • Kim, Sang Woo (Department of Pediatrics, Inje University Sanggye Paik Hospital, Inje University College of Medicine)
  • 한태희 (인제대학교 의과대학 진단검사의학과교실) ;
  • 정주영 (인제대학교 의과대학 소아과학교실) ;
  • 성희경 (인제대학교 생명과학대학교, 생명과학연구소) ;
  • 김상우 (인제대학교 의과대학 소아과학교실)
  • Received : 2006.05.24
  • Accepted : 2006.07.05
  • Published : 2006.09.15
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose : The purpose of this study was to investigate the presence of Bartonella henselae DNA, which is known as an etiologic agent of lymphadenitis, in fleas from dogs. Methods : The Bartonella henselae infection was investigated in 42 fleas from 22 dogs in Korea. By using seminested PCR targeting pap31 gene, B. henselae DNA was amplified from fleas. Results : B. henselae DNA was detected in seven fleas (7 of 42 fleas, 16.7 percent) from four dogs (4 of 22 dogs, 18.2 percent). To confirm these findings, we performed sequencing and identified the seven PCR products. Sequence analysis revealed that six sequences belonged to Huston-1 genogroup and one sequence to Marseille genogroup. Conclusion : These results may suggest that dogs could be an important source of B. henselae infection in children in Korea. This is the first report about the detection of B. henselae in fleas from dogs in Korea.

목 적 : 소아 임파선염의 흔한 원인균의 하나로 잘 알려진 Bartonella henselae는 주로 고양이 벼룩에 감염된 고양이와의 접촉을 통하여 병을 일으키는 것으로 알려졌지만, 최근에는 개도 보유 숙주임이 확인되었다. 국내의 CSD증례 보고들에서는 고양이보다 개와 접촉한 경우가 더 많은 것으로 보고되었다. 하지만 국내에서는 개나 고양이 또는 매개체인 고양이 벼룩 등에서 B. henselae가 확인된 경우가 아직 없었다. 이에 저자들은 개에게서 얻은 벼룩을 대상으로 B. henselae DNA가 존재하는지 알아보기 위하여 본 연구를 시행하였다. 방 법 : 전체 22마리의 개에서 얻은 총 42마리의 벼룩을 대상으로 연구를 시행하였다. 각각의 벼룩에서 DNA를 추출한 다음 B. henselae의 pap31 부위 유전자를 이용한 시발체를 사용하여 seminested PCR을 시행하였다. 결 과 : B. henselae DNA는 전체 22마리 중 4마리의 개(18.2%)에서 추출되었던 벼룩 42마리 중 7마리(16.7%)에서 확인되었다. PCR 결과를 확인하기 위하여 7개의 PCR 산물에 대한 염기서열 분석을 시행하였다. 염기서열 분석에 의하면 6개는 Huston-1 유전자형이었으며, 한 개는 Marseille 유전자형이었다. 결 론 : 국내 개에서 추출된 벼룩에서 Houston-1유전자형과 Marseille 유전자형을 포함한 B. henselae DNA가 존재하는 것으로 확인할 수 있었다. 국내에서 개가 CSD의 중요한 감염 경로일 가능성을 제시한 점에 의의가 있지만, 충분한 수의 고양이 및 개에서 얻은 혈청과 벼룩을 대상으로 한 추가적인 연구가 필요할 것으로 생각된다.

Keywords

References

  1. Anderson BE, Neuman MA. Bartonella spp. as emerging human pathogens. Clin Microbiol Rev 1997;10:203-19
  2. Rolain JM, Franc M, Davoust B, Raoult D. Molecular detection of Bartonella quintana, B. koehlerae, B. henselae, B. clarridgeiae, Rickettsia felis, and Wolbachia pipientis in cat fleas, France. Emerg Infect Dis 2003;9:338-42 https://doi.org/10.3201/eid0903.020278
  3. Skerget M, Wenisch C, Daxboeck F, Krause R, Haberl R, Stuenzner D. Cat or dog ownership and seroprevalence of ehrlichiosis, Q fever, and cat-scratch disease. Emerg Infect Dis 2003;9:1337-40 https://doi.org/10.3201/eid0910.030206
  4. Rolain JM, Locatelli C, Chabanne L, Davoust B, Raoult D. Prevalence of Bartonella clarridgeiae and Bartonella henselae in domestic cats from France and detection of the organisms in erythrocytes by immunofluorescence. Clin Diagn Lab Immunol 2004;11:423-5
  5. La Scola B, Davoust B, Boni M, Raoult D. Lack of correlation between Bartonella DNA detection within fleas, serological results, and results of blood culture in a Bartonella-infected stray cat population. Clin Microbiol Infect 2002;8:345-51 https://doi.org/10.1046/j.1469-0691.2002.00434.x
  6. Shaw SE, Kenny MJ, Tasker S, Birtles RJ. Pathogen carriage by the cat flea Ctenocephalides felis (Bouche) in the United Kingdom. Vet Microbiol 2004;102:183-8 https://doi.org/10.1016/j.vetmic.2004.06.013
  7. Murano I, Tsuneoka H, Iino H, Kamei T, Nakamura I, Tsukahara M. Two patients with Bartonella henselae infection from a dog. Kansenshogaku Zasshi 2001;75:808-11
  8. Gundi VA, Bourry O, Davous B, Raoult D, La Scola B. Bartonella clarridgeiae and B. henselae in dogs, Gabon. Emerg Infect Dis 2004;10:2261-2
  9. Solano-Gallego L, Bradley J, Hegarty B, Sigmon B, Breitschwerdt E. Bartonella henselae IgG antibodies are prevalent in dogs from southeastern USA. Vet Res 2004;35:585-95 https://doi.org/10.1051/vetres:2004034
  10. Chang HM. Pet products in South Korea. http://weekly. hankooki.com/whan /200306/w2003060410572161510.htm (in Korean)
  11. American Pet Association. Fun pet statistics. http://www. apapets.com/petstats2.htm
  12. Chae MB, Lee JY, Kwak YG, Park SH, Lim HJ, Park SW, et al. Prevalence of antibodies to Bartonella henselae and Bartonella quintana in Korean Patients with Lymphadenopathy. Korean J Infect Dis 2002;34:305-10 https://doi.org/10.1080/00365540110080151
  13. Chung JY, Koo JW, Kim SW, Yoo YS, Han TH, Lim SJ. A case of cat scratch disease confirmed by polymerase chain reaction for Bartonella henselae DNA. Korean J Pediatr 2005;48:789-92
  14. Chung JY, Han TH, Kim BN, Yoo YS, Lim SJ. Detection of Bartonella henselae DNA by polymerase chain reaction in a patient with cat scratch disease. Korean J Med Sci 2005;20:888-91 https://doi.org/10.3346/jkms.2005.20.5.888
  15. Menier K, Beaucournu JC. Taxonomic study of the genus Ctenocephalides (Stiles & Collins, 1930) by using aedeagus characters. J Med Entomol 1998;35:883-90 https://doi.org/10.1093/jmedent/35.5.883
  16. Zeaiter Z, Fournier PE, Raoult D. Genomic variation of Bartonella henselae strains detected in lymph nodes of patients with cat scratch disease. J Clin Microbiol 2002;40:1023-30 https://doi.org/10.1128/JCM.40.3.1023-1030.2002
  17. Clarithers HA. Cat-scratch disease: an overview based on a study of 1,200 patients. Am J Dis Child 1985;139:1124-33 https://doi.org/10.1001/archpedi.1985.02140130062031
  18. Jackson LA, Perkins BA, Wenger JD. Cat scratch disease in the United States. Am J Public Health 1993;83:1707-11 https://doi.org/10.2105/AJPH.83.12.1707
  19. Murakami K, Tsukahara M, Tsuneoka H, Iino H, Ishida C, Tsujino K, et al. Cat scratch disease : analysis of 130 seropositive cases. J Infect Chemother 2002;8:349-52 https://doi.org/10.1007/s10156-002-0194-6
  20. Cruz-Vazquez C, Castro Gamez E, Parada Fernandez M, Ramos Parra M. Seasonal occurrence of Ctenocephalides felis felis and Ctenocephalides canis (Siphonaptera : Pulicidae) infesting dogs and cats in an urban area in Cuernavaca, Mexico. J Med Entomol 2001;38:111-3 https://doi.org/10.1603/0022-2585-38.1.111
  21. Alcaino HA, Gorman TR, Alcaino R. Flea species from dogs in three cities of Chile. Vet Parasitol 2002;105:261-5 https://doi.org/10.1016/S0304-4017(01)00626-4
  22. Azad AF, Radulovic S, Higgins JA, Noden BH, Troyer JM. Flea-borne rickettsioses : ecologic considerations. Emerg Infect Dis 1997;3:319-27 https://doi.org/10.3201/eid0303.970308
  23. Kim CK, Kim JY, Yi YH, Lee MJ, Cho MR, Shah DH, et al. Detection of Bartonella species from ticks, mites and small mammals in Korea. J Vet Sci 2005;6:327-4
  24. Kelly PJ, Meads N, Theobald A, Fournier P, Raoult D. Rickettsia felis, Bartonella henselae, and B. clarridgeiae, New Zealand. Emerg Infect Dis 2004:10;967-8 https://doi.org/10.3201/eid1005.030986
  25. Barnes A, Bell SC, Isherwood DR, Bennett M, Carter SD. Evidence of Bartonella henselae infection in cats and dogs in the United Kingdom. Vet Rec 2000;147:673-7
  26. Birtles RJ, Laycock G, Kenny MJ, Shaw SE, Day MJ. Prevalence of Bartonella species causing bacteraemia in domesticated and companion animals in the United Kingdom. Vet Rec 2002;151:225-9 https://doi.org/10.1136/vr.151.8.225