Molecules and Cells

  • 제22권1호
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  • Pages.21-29
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  • 2006
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

Hydrogen Peroxide Induces Apoptosis of BJAB Cells Due to Formation of Hydroxyl Radicals Via Intracellular Iron-mediated Fenton Chemistry in Glucose Oxidase-mediated Oxidative Stress

  • Lee, Jeong-Chae (Laboratory of Cell Biology, Department of Orthodontics and Institute of Oral Biosciences, Chonbuk National University) ;
  • Son, Young-Ok (Laboratory of Cell Biology, Department of Orthodontics and Institute of Oral Biosciences, Chonbuk National University) ;
  • Choi, Ki-Choon (Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Korea University) ;
  • Jang, Yong-Suk (Research Center of Bioactive Materials, Chonbuk National University)
  • 투고 : 2006.02.05
  • 심사 : 2006.06.13
  • 발행 : 2006.08.31
⟨ 이전 논문 다음 논문 ⟩

초록

The aim of this study was to determine if hydrogen peroxide ($H_2O_2$) generated by glucose oxidase (GO) induces apoptosis or necrosis of BJAB cells and which radical is the direct mediator of cell death. We found that GO produced $H_2O_2$ continuously in low concentrations, similar to in vivo conditions, and decreased proliferation and cell viability in a dose-dependent manner. The GO-mediated cytotoxicity resulted from apoptosis, and was confirmed by monitoring the cells after H33342/Annexin V/propidium iodide staining. Decreases of mitochondrial membrane potential and intracellular glutathione level were found to be critical events in the $H_2O_2$-mediated apoptosis. Additional experiments revealed that $H_2O_2$ exerted its apoptotic action through the formation of hydroxyl radicals via the Fenton rather than the Haber-Weiss reaction. Moreover, intracellular redox-active iron, but not copper, participated in the $H_2O_2$-mediated apoptosis.

키워드

과제정보

연구 과제 주관 기관 : National Livestock Research Institute

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