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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Stability Evaluation of Vitamin-C Inclusion Complexes Prepared using Supercritical ASES Process

초임계 ASES 공정으로 제조된 Vitamin-C 포접복합체의 안정성 평가

  • Yang, Jun-Mo (Department of Chemical and Biochemical Engineering, The University of Suwon) ;
  • Kim, Seok-Yun (Department of Chemical and Biochemical Engineering, The University of Suwon) ;
  • Han, Ji-Hyun (Department of Chemical and Biochemical Engineering, The University of Suwon) ;
  • Jung, In-Il (Department of Chemical and Biochemical Engineering, The University of Suwon) ;
  • Ryu, Jong-Hoon (Department of Chemical and Biochemical Engineering, The University of Suwon) ;
  • Lim, Gio-Bin (Department of Chemical and Biochemical Engineering, The University of Suwon)
  • 양준모 (수원대학교 공과대학 화공생명공학과) ;
  • 김석윤 (수원대학교 공과대학 화공생명공학과) ;
  • 한지현 (수원대학교 공과대학 화공생명공학과) ;
  • 정인일 (수원대학교 공과대학 화공생명공학과) ;
  • 유종훈 (수원대학교 공과대학 화공생명공학과) ;
  • 임교빈 (수원대학교 공과대학 화공생명공학과)
  • Published : 2006.04.28
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Abstract

A supercritical fluid process, called aerosol solvent extraction system(ASES), is especially suitable to the pharmaceutical, cosmetic and food industries due to its environmentally-friendly, non-toxic and residual solvent-free properties. In particular, the application of the ASES process to the processing of thermo-labile bioactive compounds has received attention of many scientists and engineers because of its low-temperature operating conditions. Unstable substances such as Vitamin-C and Vitamin-A can be effectively protected from degradation during the preparation process, because the ASES process is free from oxygen and moisture. In this study, Vitamin-C was formulated with 2-hydroxypropyl-${\beta}$-cyclodextrin (HP-${\beta$-CD) for enhancement of Vitamin-C stability and bioavailability using the ASES process. To investigate the influence of the preparation process on the stability of Vitamin-C, Vitamin-C/HP-${\beta}$-CD inclusion complexes were prepared using both conventional solvent evaporation method and ASES process, and stored in a 50 mM phosphate buffer solution of pH 7.0 at $25^{\circ}C$ for 24 hours. From the experimental results, the stability of the Vitamin-C/HP-${\beta}$-CD inclusion complex prepared from the ASES process was found to be much higher than that of pure Vitamin-C and the Vitamin-C/HP-${\beta}$-CD inclusion complex prepared by the solvent evaporation method. The stability of Vitamin-C was observed to increase with the decrease of temperature at a constant pressure or with the increase of pressure at a constant temperature.

본 연구에서는 초임계 ASES 공정을 이용하여 인체내 및 피부에 여러 가지로 유익한 대표적 생리활성물질인 Vitamin-C의 불안정성을 극복하기 위하여 HP-${\beta}$-CD와의 포접복합체를 제조하여 수용액상에서의 안정성을 분석하였다. X-선 회절을 이용한 Vitamin-C와 HP-${\beta}$-CD의 결정성 분석으로 초임계 ASES 공정을 통하여 포접복합체가 용이하게 형성될 수 있음을 확인하였다. HP-${\beta}$-CD가 Vitamin-C의 안정성을 향상시키는 방법으로 이용될 수 있음을 확인하였으며, $25{\pm}0.1^{\circ}C$의 온도를 유지한 pH 7.0의 50 mM 인산완충용액 상에서 순수한 Vitamin-C, 물리적인 혼합물 및 용매증발법과 초임계 ASES 공정을 이용하여 제조된 포접복합체의 Vitamin-C 겉보기 1차 분해 속도 상수는 각각 $1.45{\times}10^{-2}h^{-1},\;1.41{\times}10^{-2}h^{-1},\;1.34{\times}10^{-2}h^{-1},\;0.20{\times}10^{-2}h^{-1}$로 초임계 ASES 공정으로 제조된 포접복합체의 경우 Vitamin-C의 안정성이 매우 크게 향상되는 것을 확인하였다.

Keywords

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