KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Production of Extracellular Polysaccharide by Perfusion Culture of Angelica gigas Nakai Suspension Cells

배지교환식 고농도 배양에 의한 참당귀 현탁세포 유래 ECP 생산

  • Kim, Young-Hwa (Department of Biological Engineering, Inha University) ;
  • Kim, Ik-Hwan (School of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Dong-Il (Department of Biological Engineering, Inha University)
  • 김영화 (인하대학교 공과대학 생물공학과) ;
  • 김익환 (고려대학교 생명공학원) ;
  • 김동일 (인하대학교 공과대학 생물공학과)
  • Published : 2006.10.30
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Abstract

High-density perfusion cultivation was performed to produce extracellular polysaccharide(ECP) as immunostimulating agents in suspension cell cultures of Angelica gigas Nakai. In batch culture, the maximum cell density was 16.8 gDCW/L at day 6 and 0.9 g/L of ECP was obtained at day 8. When the medium exchange was started at the fifth day after inoculation for the perfusion culture, high concentration of the cells at 23.8 gDCW/L could be achieved with continuous production of ECP. Treatments of ultrasound and Pluronic F-68 were found to be helpful for the secretion of intracellular ECP into the culture medium.

본 연구에서는 참당귀 현탁세포배양에 의한 면역 증강성 ECP의 생산을 증진시키기 위하여 배지교환식 배양을 수행하였으며, ECP의 분비를 촉진하는 초음파 처리와 Pluronic F-68의 영향을 조사하였다. 참당귀의 현탁세포배양시, 최대 세포생장은 6일째에 16.8 g DCW/L였고, ECP의 생산은 세포생장과 함께 증가하다가 8일째에 최고 0.9 g/L가 생산되었다. 식물세포 고농도배양법인 배지교환식 배양을 적용해, 초기 당 농토를 높이고 접종 후 5일부터 연속적으로 배지교환을 해주어 23.8 gDCW/L의 고농도의 현탁세포를 얻을 수 있었다. 초음파 처리 및 Pluronic F-68의 첨가는 참당귀 세포의 세포막의 투과성을 증진시켜 ECP의 생산성을 높임과 동시에 세포 내의 다당의 배지로의 배출을 유도한 것으로 판단된다.

Keywords

References

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