Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Hepatopotective Effects of Black Rice on Superoxide Anion Radicals in HepG2 Cells

  • Shim, Sang-In (Division of Plant Resources and Environment, Gyeongsang National University) ;
  • Chung, Jin-Woong (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jeong-Min (Department of Food and Nutrition, Nambu University) ;
  • Hwang, Kwon-Tack (Department of Food and Nutrition, Nambu University) ;
  • Sone, Jin (Jeonnam Innovation Agency) ;
  • Hong, Bum-Shik (Department of Food and Biotechnology, Korea University) ;
  • Cho, Hong-Yon (Department of Food and Biotechnology, Korea University) ;
  • Jun, Woo-Jin (Department of Food and Nutrition, Human Ecology Research Institute, Chonnam National University)
  • Published : 2006.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Cyanidin 3-glucoside (C3G) isolated from black rice was investigated for hepatoprotective effects in HepG2 cells under oxidative stress. When an increase in the production of reactive oxygen species (ROS) was induced by gramoxone, cell viability was drastically decreased by 42%. However, in the presence of C3G, no hepatocytic damage was observed in HepG2 cells treated with gramoxone. C3G was found to manifest a stronger scavenging effect (91%) on superoxide anion radical ($O_2\;^{.-}$) than any of the other natural and synthetic antioxidants. Results suggest that C3G from black rice possesses hepatoprotective effects in vitro, which may be, at least in part, due to $O_2\;^{.-}$ scavenging.

Keywords

References

  1. Ma J, Li Y, Ye Q, Li J, Hua Y, Ju D, Zhang D, Copper R, Chang M. Constituents of red yeast rice, a traditional Chinese food and medicine. J. Agr. Food Chem. 48: 5220-5225 (2000) https://doi.org/10.1021/jf000338c
  2. Meiers S, Kemeny M, Weyand U, Gastpar R, von Angerer E, Marko D. The anthocyanidins cyanidin and delphinidin are potent inhibitors of the epidermal growth-factor receptor. J. Agr. Food Chem. 49: 958-962 (2001) https://doi.org/10.1021/jf0009100
  3. Dangles O, Fargeix G, Dufour C. Antioxidant properties of anthocyanins and tannins: a mechanistic investigation with catechin and the 3',4',7-trihydroxyflavylium ion. J. Chem. Soc. Perk. T. 2 8: 1653-1663 (2000)
  4. Oh JK, Kim SJ, Imm JY. Antioxidative effect of anthocyanins in water-in-oil microemulsion system. Food Sci. Biotechnol. 15: 283-288 (2006)
  5. Chung HK, Choi CS, Park WJ, Kang MH. Radical scavenging activity of grape-seed extracts prepared from different solvents. Food Sci. Biotechnol. 14: 715-721 (2005)
  6. Sawa T, Akaike T, Maeda H. Tyrosine nitration by peroxynitrite formed nitric oxide and superoxide generated by xanthine oxidase. J. Biol. Chem. 275: 32467-32474 (2000) https://doi.org/10.1074/jbc.M910169199
  7. Jagetia GC, Reddy TK, Venkatesha VA, Kedlaya R. Influence of naringin on ferric iron induced oxidative damage in vitro. Clin. Chim. Acta 347: 189-197 (2004) https://doi.org/10.1016/j.cccn.2004.04.022
  8. Ho IN, Lee YH, Park JS, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY. Protective effects of aucubin isolated from Eucommia ulmoides against UVB-induced oxidative stress in human skin fibroblast. Biol. Pharm. Bull. 28: 1244-1248 (2005) https://doi.org/10.1248/bpb.28.1244
  9. Mitsuyoshi H, Nakashima T, Sumida Y, Yoh T, Nakajima Y, Ishikawa H, Inaba K, Sakamoto Y, Okanoue T, Kashima K. Ursodeoxycholic acid protects hepatocytes against oxidative injury via induction of antioxidants. Biochem. Bioph. Res. Co. 263: 537-542 (1999) https://doi.org/10.1006/bbrc.1999.1403
  10. Tsuda T, Ohshima K, Kawakishi S, Osawa T. Antioxidative pigments isolated form the seeds of Phaseolus vulgaris L. J. Agr. Food Chem. 42: 248-251 (1994) https://doi.org/10.1021/jf00038a004
  11. Ryu SN, Park SZ, Ho CT. High performance liquid chromatographic determination of anthocyanin pigments in some varieties of black rice. J. Food Drug Anal. 6: 729-736 (1998)
  12. Rochem NW, Rodger GH, Hatfield SM, Glasebrook AL. An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J. Immunol. Methods 142: 257-265 (1991) https://doi.org/10.1016/0022-1759(91)90114-U
  13. Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J. Agr. Food Chem. 51: 6509-6515 (2003) https://doi.org/10.1021/jf0343074
  14. Slimestad R, Andersen OM. Cyanidin 3-(2-glucosylgalactoside) and other anthocyanins from fruits of Comus suecica. Phytochemistry 49: 2163-2166 (1998) https://doi.org/10.1016/S0031-9422(98)00397-5
  15. Tsuda T, Horio F, Osawa T. Absorption and metabolism of cyanidin 3-0-$\beta$-D-glucoside in rats. FEBS Lett. 449: 179-182 (1999) https://doi.org/10.1016/S0014-5793(99)00407-X
  16. Wang J, Mazza G Inhibitory effects of anthocyanins and other phenolics compounds on nitric oxide production in LPS/IFN-$\gamma$-activated Raw 264.7 macrophages. J. Agr. Food Chem. 50: 850-857 (2002) https://doi.org/10.1021/jf010976a
  17. Wang J, Mazza G Effects of anthocyanins and other phenolics compounds on the production of tumor necrosis factor $\gamma$ in LPS/IFN-$\gamma$-activated Raw 264.7 macrophages. J. Agr. Food Chem. 50: 4183-4189 (2002) https://doi.org/10.1021/jf011613d
  18. Rossi AL, Blostein-Fujii A, DiSilvestro RA. Soy beverage consumption by young men: increased plasma total antioxidant status and decreased acute, exercise-induced muscle damage. J. Nutr. Func. Med. Foods 3: 279-291 (2000)
  19. Cuthbert C, Wang Z, Zhang X. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960 (1997) https://doi.org/10.1074/jbc.272.23.14954
  20. Choi HS, Kim JW, Cha YN, Kim C. A quantitative nitroblue tetrazolium assay for determining intracellular superoxide anion production in phagocytic cells. J. Immunoassay Immunochem. 27: 31-44 (2005)
  21. Lass A, Sohal RS. Effect of coenzyme Q10 and $\alpha$-tocopherol content of mitochondria on the production of superoxide anion radicals. FASEB J. 14: 87-94 (2000) https://doi.org/10.1096/fasebj.14.1.87
  22. Joen TI, Hwang So, Park NG, Jung YR, Shin SI, Choi SD, Park DK. Antioxidative effect of chitosan on chronic carbon tetrachloride induced hepatic injury in rats. Toxicology 187: 67-73 (2003) https://doi.org/10.1016/S0300-483X(03)00003-9