Effect of a Static Magnetic Field on Susceptibility to Ethanol-Induced Hepatic Dysfunction in Rats

  • Park, Kap-Joo (Department of Biological Sciences, Konkuk University) ;
  • Kim, Eun-Jung (Department of Culinary and Food Service, Sejong University) ;
  • Cho, Myung-Hwan (Department of Biological Sciences, Konkuk University) ;
  • Lee, Jae-Seok (Department of Biological Sciences, Konkuk University)
  • Published : 2006.12.31

Abstract

To determine whether alcohol-treated rat liver cells can be protected by a static magnetic field (SMF), we analyzed the blood chemistry and histology of hepatic tissue removed from alcohol-exposed rats that had been exposed to a static magnetic field. The rats were exposed to a 0.3 tesla (3,000 gauss) magnetic field (MF) for 24 hr daily for 5 weeks with appropriate controls. Glutamic pyruvic transaminase activity and the triglyceride levels in animals exposed to the north (N) or south (S) pole of the MF decreased significantly (p<0.01 and p<0.05, respectively) compared with negative control animals with alcohol exposure. A histological examination of hepatic tissue revealed a moderate to severe accumulation of fat vacuoles of various sizes in the cytoplasm of the hepatocytes of animals in the negative control group throughout the study; whereas in groups exposed to the MF poles, fewer fat vacuoles were seen compared with the negative control group. Electron microscopic observations showed that exposure to the N or S pole protected organelles, including the nucleus, from damage during exposure to this toxic agent, as indicated by the fact that the nucleus and the mithochondria virtually retained their shape throughout this study. These results suggest that exposure to a SMF could be an excellent way of protecting against alcohol-induced damage to the rat liver cell.

Keywords

References

  1. Weinber A, Nyska A, Oiler S. Treatment of experimental inflammatory synovitis with continous magnetic field. Israel J. Med. Sci. 32: 1197-1201 (1996)
  2. Hong C-Z , Lin JC, Bender LF, Schaeffer JN, Meltzer RJ, Causin P. Magnetic necklace: its therapeutic effectiveness on neck and shoulder pain. Arch. Phys. Med. Rehab. 63: 462-466 (1982)
  3. Vallbina C, Haz1eswood CF, Jurida G Response of pain to static magnetic fields in postpo1io patients: a double-blind pilot study. Arch. Phys. Med. Rehab. 78: 1200-1203 (1997) https://doi.org/10.1016/S0003-9993(97)90332-4
  4. Nakagawa K. Magnetic deficiency syndrome and magnetic treatment. Japan Med. J. 2745: 24-32 (1976)
  5. Nakagawa K. Study on clinical effects of the magnetic necklace: Isuzu Hospital, Tokyo, Japan. TDK Magneto Medical Publication Series No 1. TDK Magneties Corp. Beverly Hills, CA, USA. pp. 1-12 (1975)
  6. Considine D. Van Nostrand's Scientific Encyclopedia. ITP, New York, NY, USA. p.1534 (1995)
  7. Roland D, Ferder M, Kothuru R, Faierman T, Stranch B. Effects of magnetic energy on a microsurgically transferred vessel. Plast. Reconstr. Surg. 105: 1371-1374 (2000) https://doi.org/10.1097/00006534-200004040-00016
  8. Hansen KM. Some observations with view to possible influence of magnetism upon human organism. Acta. Med. Scand. 97: 339-364 (1938) https://doi.org/10.1111/j.0954-6820.1938.tb09980.x
  9. Hong C-Z. Static magnetic field influence on human nerve function. Arch. Phys. Med. Rehab. 68: 162-164 (1987)
  10. Davis AR, Rawls WC Jr. The Magnetic Effect. Exposition Press, Smithtown, NY, USA. pp.118-145 (1980)
  11. Ohlsen JH. Continuous exposure to strong magnetic fields. Brit. Med. J. 307: 891-895 (1993) https://doi.org/10.1136/bmj.307.6909.891
  12. Lee JS, Kim HY, Park KJ, Lee HH. Effects of soybean embryo on liver protection and lipid metabolism of alcohol-fed rats. Food Sci. Biotechnol. 14: 102-107 (2005)
  13. Park KJ, Kim HY, Chang BJ, Lee HH. Ameliorative effects of soy 11s protein on liver damage and hyperlipidemia in alcohol-fed rats. Bio. Pharm. Bull. 27: 1636-1641 (2004) https://doi.org/10.1248/bpb.27.1636
  14. Lee JS, Ahn KH, Park KJ. Ameliorative effects of pine needle oil on liver protection and lipid metabolism of alcohol fed rats. Food Sci. Biotechnol. 14: 99-101 (2005)
  15. Park KJ, Lee MJ, Kang H, HH Lee. A medicinal herb complex protects liver cell damage induced by alcohol. Bio. Pharm. Bull. 25: 451-1455 (2002)
  16. Sanders JB, Wodak AD, Williams R. What determines susceptibility to liver damage from alcohol? Discussion paper. J. Royal Soc. Med. 77: 204-216 (1984) https://doi.org/10.1177/014107688407700311
  17. Cho SH, Ha TY. In vitro and in vivo effects of prosomillet and sorghum on cholesterol metabolism. Food Sci. Biotechnol. 12: 485-490 (2004)
  18. Kerai MDJ, Waterfield CJ, Kenyon SH, Asker DS, Timbrell JA. The effect of taurine depletion by $\beta$-alanine treatment on the susceptibility to ethanol-induced hepatic dysfunction in rats. Alcohol 36: 29-38 (2000)
  19. Martinez FE, Martinez M, Padovani CR, Bustos-Obregon E. Morphology of testis and epididymis in an ethanol-drinking rat strain (UchA and UchB). J. Submicr. Cytol. Path. 132: 175-184 (2000)
  20. Giannessi F, Giambelluca MA, Ruffoli R. The ultrastructural localization of NADPH-diaphorase enzymatic activity in the Leydig cells of mouse effects of ethanol administration. Ital. J. Anat. Embryol. 103: 153-165 (1998)
  21. Morrow DA, Hillman D, Dade AW, Kitchen H. Clinical investigation of a dairy herd with the fat cow syndrome. J. Am. Vet. Med. Assoc. 174: 161-169 (1979)
  22. Herdt TH, Wensing T, Haagsman HP, Van Golde LMG, Breukink HJ. Hepatic triacylglycerol synthesis during a period of fatty liver development in sheep. J. Anim. Sci. 66: 1997-2006 (1998) https://doi.org/10.2527/jas1988.6681997x