BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
권호 표지
DOI QR코드

DOI QR Code

Mistletoe Lectin (Viscum album coloratum) Modulates Proliferation and Cytokine Expressions in Murine Splenocytes

  • Lyu, Su-Yun (Immune Modulation Research Group, School of Pharmacy, University of Nottingham) ;
  • Park, Won-Bong (College of Natural Sciences, Seoul Women's University)
  • 투고 : 2006.04.02
  • 심사 : 2006.06.19
  • 발행 : 2006.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

It is well documented that an extract of European mistletoe has a variety of biological effects, such as the stimulation of cytokine production from immune cells, and additional immunoadjuvant activities. While the European mistletoe has been studied intensively, we know less about Korean mistletoe as a therapeutic plant, especially as a possible immunomodulating drug. This study will investigated the effects of Korean mistletoe lectin (Viscum album L. var. coloratum agglutinin, VCA) on murine splenocytes to investigate whether VCA acts as an immunomodulator, which could lead to improved immune responses in these cells. The results showed that VCA inhibited cell proliferation at higher concentrations (at 1-8 ng/ml) and enhanced cell proliferation at lower concentrations (at 4-32 pg/ml). Further studies were carried out to determine if the pro-proliferative or anti-proliferative activity exhibited by VCA was correlated with cytokine secretion. Consequently, interferon (IFN)-$\gamma$ secretion was decreased in concanavalin A (ConA)-stimulated murine splenocytes by VCA (4-64 ng/ml), but there was no change in IL-4 levels. This suggests that VCA has the ability to modulate murine splenocyte proliferation and can possibly act on the balance of Th1/Th2 cellular immune responses.

키워드

참고문헌

  1. Bussing, A. (1997) Mistletoe therapy and immunological research. Anticancer Drugs 8, 65 https://doi.org/10.1097/00001813-199704001-00015
  2. Bussing, A., Suzart, K., Bergmann, J., Pfüller, U. and Schweizer, K. (1996) Induction of apoptosis in human lymphocytes treated with Viscum album L. is mediated by the mistletoe lectins. Cancer Lett. 109, 33-38 https://doi.org/10.1016/S0304-3835(96)04401-1
  3. Choi, S. H., Lyu, S. Y. and Park, W. B. (2004) Mistletoe lectin induces apoptosis and telomerase inhibition in human A253 cancer cells through dephosphorylation of Akt. Arch. Pharm. Res. 27, 68-76 https://doi.org/10.1007/BF02980049
  4. Franz, H., Ziska, P. and Kindt, A. (1981) Isolation and properties of three lectins from mistletoe (Viscum album L.). Biochem. J. 195, 481-484 https://doi.org/10.1042/bj1950481
  5. Gaultier, R. and Chevalier, J. (1907) Action physiologique du gui (Viscum album). Comptes Rendues de l'Academie des Sciences de Paris 145, 941-942
  6. Gleichmann, E., Kimber, I. and Purchase, I. F. (1989) Immunotoxicology: suppressive and stimulatory effects of drugs and environmental chemicals on the immune system. A discussion. Arch. Toxicol. 63, 257-273 https://doi.org/10.1007/BF00278639
  7. Hajto, T., Hostanska, K., Fischer, J. and Saller, R. (1997) Immunomodulatory effects of Viscum album agglutinin-I on natural immunity. Anticancer Drugs 8, 543-546
  8. Hajto, T., Hostanska, K., Frei, K., Rordorf, C. and Gabius, H. J. (1990) Increased secretion of tumor necrosis factor a, interleukin 1, and interleukin 6 by human mononuclear cells exposed to $\beta$-galactoside-specific lectin from clinically applied mistletoe extract. Cancer Res. 50, 3322-3326
  9. Hajto, T., Hostanska, K. and Gabius, H. J. (1989) Modulatory potency of the $\beta$-galactoside-specific lectin from mistletoe extract (Iscador) on the host defense system in vivo in rabbits and patients. Cancer Res. 49, 4803-4808
  10. Hostanska, K., Hajto, T., Spagnoli, G. C., Fischer, J., Lentzen, H. and Hermann, R. (1995) A plant lectin derived from Viscum album induces cytokine gene expression and protein production in cultures of human peripheral blood mononuclear cells. Natural Immunity 14, 295-304
  11. Kay, T. W., Campbell, I. L. and Harrison, L. C. (1991) Characterization of pancreatic T lymphocytes associated with beta cell destruction in the non-obese diabetic (NOD) mouse. J. Autoimmun. 4, 263-276 https://doi.org/10.1016/0896-8411(91)90023-6
  12. Khwaja, T. A., Dias, C. B. and Pentecost, S. (1986) Recent studies on the anticancer activities of mistletoe (Viscum album) and its alkaloids. Oncology 43, 42-50 https://doi.org/10.1159/000226419
  13. Kuttan, G., Vasudevan, D. M. and Kuttan, R. (1992) Effect of a preparation from Viscum album on tumor development in vitro and in mice. J. Ethnopharmacol. 29, 35-41 https://doi.org/10.1016/0378-8741(90)90095-B
  14. Li, S. Z. (1975) Material medica principles: describes 1,892 kinds of medical material in 1578 AD; in The Great Pharmacopoeia, Ben-Cao and Gang-mu (eds.), pp. 145-168, University of California Press, Beijing. P. R, China
  15. Lyu, S. Y., Choi, S. H. and Park, W. B. (2002) Korean mistletoe lectin-induced apoptosis in hepatocarcinoma cells is associated with inhibition of telomerase via mitochondrial controlled pathway independent of p53. Arch. Pharm. Res. 25, 93-101 https://doi.org/10.1007/BF02975269
  16. Lyu, S. Y., Park, S. M., Choung, B. Y. and Park, W. B. (2000) Comparative study of Korean (Viscum album var. coloratum) and European mistletoes (Viscum album). Arch. Pharm. Res. 23, 592-598 https://doi.org/10.1007/BF02975247
  17. Lyu, S. Y., Park, W. B., Choi, K. H. and Kim, W. H. (2001) Involvement of caspase-3 in apoptosis induced by Viscum album var. coloratum agglutinin in HL-60 cells. Biosci. Biotechnol.Biochem. 65, 534-541 https://doi.org/10.1271/bbb.65.534
  18. Mueller, E. A. and Anderer, F. A. (1990) A Viscum album oligosaccharide activating human natural cytotoxicity is an interferon gamma inducer. Cancer Immunol. Immunother. 32, 221-227 https://doi.org/10.1007/BF01741704
  19. O'Garra, A., Macatonia, S. E., Hsieh, C. S. and Murphy, K. M. (1993) Regulatory role of IL4 and other cytokines in T helper cell development in an alpha beta TCR transgenic mouse system. J. Immunol. 151, 4200-4208
  20. O'Garra, A. and Murphy, K. (1993) T-cell subsets in autoimmunity. Curr. Opin. Immunol. 5, 880-886 https://doi.org/10.1016/0952-7915(93)90100-7
  21. Park, J. H., Hyun, C. K. and Shin, H. K. (1998a) Cytotoxicity of heat-treated Korean mistletoe. Cancer Lett. 126, 43-48 https://doi.org/10.1016/S0304-3835(97)00526-0
  22. Park, W. B., Han, S. K., Lee, M. H. and Han, K. H. (1997) Isolation and characterizaton of lectins from stem and leaves of Korean mistletoe (Viscum album L. var. coloratum) by affinity chromatography. Arch. Pharm. Res. 20, 306-312 https://doi.org/10.1007/BF02976191
  23. Park, W. B., Ju, Y. J. and Han, S. K. (1998b) Isolation and characterization of $\beta$-galactoside specific lectin from Korean mistletoe (Viscum album L. var coloratum) with lactose-BSASepharose 4B and changes of lectin conformation. Arch. Pharm. Res. 21, 429-435 https://doi.org/10.1007/BF02974638
  24. Park, W. B., Lyu, S. Y., Kim, J. H., Choi, S. H., Chung, H. K., Ahn, S. H., Hong, S. Y., Yoon, T. J. and Choi, M. J. (2001) Inhibition of tumor growth and metastasis by Korean mistletoe lectin is associated with apoptosis and antiangiogenesis. Cancer Biother. Radiopharm. 16, 439-447 https://doi.org/10.1089/108497801753354348
  25. Peng, H. Y., Zhang, Y. H., Han, Y. and Wang, M. (2005) Studies on the anticancer effects of total alkaloid from Viscum coloratum. Zhongguo Zhong Yao Za Zhi, 30, 381-382, 387
  26. Peumans, W. J., Verhaert, P., Pfuller, U. and Van Damme, E. J. (1996) Isolation and partial characterization of a small chitinbinding lectin from mistletoe (Viscum album). FEBS Lett. 396, 261-265 https://doi.org/10.1016/0014-5793(96)01108-8
  27. Schultze, J. L., Stettin, A. and Berg, P. A. (1991) Demonstration of specifically sensitized lymphocytes in patients treated with an aqueous mistletoe extract (Viscum album L.). Klinische Wochenschrift 69, 397-403 https://doi.org/10.1007/BF01647413
  28. Schumacher, U., Adam, E., Kretzschmar, H. and Pfuller, U. (1994) Binding patterns of mistletoe lectins I, II and III to microglia and Alzheimer plaque glycoproteins in human brains. Acta. Histochemica 96, 399-403 https://doi.org/10.1016/S0065-1281(11)80025-5
  29. Urech, K., Scher, J. M., Hostanska, K. and Becker, H. (2005) Apoptosis inducing activity of viscin, a lipophilic extract from Viscum album L. J. Pharm. Pharmacol. 57, 101-109 https://doi.org/10.1211/0022357055083
  30. Yoon, T. J., Yoo, Y. C., Choi, O. B., Do, M. S., Kang, T. B., Lee, S. W., Azuma, I. and Kim, J., B. (1995) Inhibitory effect of Korean mistletoe (Viscum album coloratum) extract on tumour angiogenesis and metastasis of haematogenous and nonhaematogenous tumour cells in mice. Cancer Lett. 97, 83-91 https://doi.org/10.1016/0304-3835(95)03956-W
  31. Yoon, T. J., Yoo, Y. C., Kang, T. B., Her, E., Kim, S. H., Kim, K., Azuma, I. and Kim, J. B. (2001) Cellular and humoral adjuvant activity of lectins isolated from Korean mistletoe (Viscum album coloratum). Int. Immunopharm. 1, 881-889 https://doi.org/10.1016/S1567-5769(01)00024-8
  32. Yoon, T. J., Yoo, Y. C., Kang, T. B., Shimazaki, K., Song, S. K., Lee, K. H., Kim, S. H., Park, C. H., Azuma, I. and Kim, J. B. (1999) Lectins isolated from Korean mistletoe (Viscum album coloratum) induce apoptosis in tumor cells. Cancer Lett. 136, 33-40 https://doi.org/10.1016/S0304-3835(98)00300-0
  33. Yoon, T. J., Yoo, Y. C., Kang, T. B. B., Y. J., Huh, C. S., Song, S. K., Lee, K. H., Azuma, I. and Kim, J. B. (1998) Prophylactic effect of Korean mistletoe (Viscum album coloratum) extract on tumor metastasis is mediated by enhancement of NK cell activity. Int. J. Immunopharmacol. 20, 163-172 https://doi.org/10.1016/S0192-0561(98)00024-1
  34. Zurawski, G. and de Vries, J. E. (1994a) Interleukin 13 elicits a subset of the activities of its close relative interleukin 4. Stem Cells 12, 169-174 https://doi.org/10.1002/stem.5530120204
  35. Zurawski, G. and de Vries, J. E. (1994b) Interleukin 13, an interleukin-4-like cytokine that acts on monocytes and B cells, but not on T cells. Immunol. Today 15, 19-26 https://doi.org/10.1016/0167-5699(94)90021-3

피인용 문헌

  1. The Efficiency ofViscum album ssp. albumandHypericum perforatumon Human Immune CellsIn Vitro vol.30, pp.3, 2008, https://doi.org/10.1080/08923970802135286
  2. Immunologic Response to Mistletoe Extract (Viscum album L.) after Conventional Treatment in Patients with Operable Breast Cancer vol.13, pp.1, 2010, https://doi.org/10.4048/jbc.2010.13.1.14
  3. Fructans from aged garlic extract produce a delayed immunoadjuvant response to ovalbumin antigen in BALB/c mice vol.34, pp.1, 2012, https://doi.org/10.3109/08923973.2011.584066
  4. Korean mistletoe lectin regulates self-renewal of placenta-derived mesenchymal stem cells via autophagic mechanisms vol.45, pp.5, 2012, https://doi.org/10.1111/j.1365-2184.2012.00839.x
  5. Liver Injury Induced by Herbal Extracts Containing Mistletoe and Kudzu vol.21, pp.3, 2015, https://doi.org/10.1089/acm.2014.0228
  6. In Vitro Immunoregulatory Effects of Korean Mistletoe Lectin on Functional Activation of Monocytic and Macrophage-Like Cells vol.30, pp.11, 2007, https://doi.org/10.1248/bpb.30.2043
  7. Inhibitory Activities of Pancreatic Lipase and Phosphodiesterase from Korean Medicinal Plant Extracts vol.26, pp.5, 2012, https://doi.org/10.1002/ptr.3644
  8. Korean mistletoe lectin promotes proliferation and invasion of trophoblast cells through regulation of Akt signaling vol.39, 2013, https://doi.org/10.1016/j.reprotox.2013.03.011
  9. The effect of subminimal inhibitory concentrations of antibiotics on virulence factors expressed byStaphylococcus aureusbiofilms vol.108, pp.4, 2010, https://doi.org/10.1111/j.1365-2672.2009.04529.x
  10. Immunomodulatory response of Cramoll 1,4 lectin on experimental lymphocytes vol.24, pp.11, 2010, https://doi.org/10.1002/ptr.3156
  11. Mistletoe lectin modulates intestinal epithelial cell-derived cytokines and B cell IgA secretion vol.32, pp.3, 2009, https://doi.org/10.1007/s12272-009-1319-6
  12. Healing activity induced by Cramoll 1,4 lectin in healthy and immunocompromised mice vol.408, pp.1-2, 2011, https://doi.org/10.1016/j.ijpharm.2011.02.011
  13. Immunomodulating effects of Korean mistletoe lectin in vitro and in vivo vol.9, pp.13-14, 2009, https://doi.org/10.1016/j.intimp.2009.09.011
  14. Viscum Album Modulates Apoptotic Related Genes in Melanoma Tumor of Mice vol.04, pp.02, 2014, https://doi.org/10.4236/ajmb.2014.42007
  15. The Identification ofin VitroProduction of Lectin from Callus Cultures of Korean Mistletoe (Viscum albumL. var.coloratum) vol.77, pp.4, 2013, https://doi.org/10.1271/bbb.120962
  16. Fermented Mistletoe Extract as a Multimodal Antitumoral Agent in Gliomas vol.2012, 2012, https://doi.org/10.1155/2012/501796
  17. European Viscum album: a potent phytotherapeutic agent with multifarious phytochemicals, pharmacological properties and clinical evidence vol.6, pp.28, 2016, https://doi.org/10.1039/C5RA27381A