The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
권호 표지

Upregulation of Renin-angiotensin, Endothelin and C-type Natriuretic Peptide in Rat Glomerulus with Bilateral Ureteral Obstruction

  • Bae, Eun-Hui (Department of Internal Medicine, Chonnam National University Medical School) ;
  • Kim, Soo-Wan (Department of Internal Medicine, Chonnam National University Medical School)
  • Published : 2006.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was designed to investigate the effects renin-angiotensin-aldosterone system (RAAS), endothelin (ET) and local natriuretic peptide (NP) system for glomerulopathy induced in the experimental bilateral ureteral obstructive rats. Sprague-Dawley male rats ($200{\sim}220g$ body weight) were bilaterally obstructed by ligation of the proximal ureters for 24 hours. Control rats were treated in the same ways, except that no ligature was made. The glomeruli were isolated from cortex by graded sieve methods, and the mRNA expressions of local renin-angiotensin system (RAS), aldosterone synthase (CYP11B2), endothelin-1 (ET-1) and NP system were determined by real-time polymerase chain reaction. Following the bilateral ureteral obstruction, the mRNA expressions of renin, angiotensin converting enzyme 1 as well as ET-1 were increased, while that of angiotensin converting enzyme 2 was not changed. The expressions of CYP11B2 and angiotensin II receptors were not changed. C-type natriuretic peptide (CNP) expression was increased, while its receptors (natriuretic peptide receptor-B) were not changed. We suggest that the upregulation of local RAS and ET playa role in the progressive glomerular injury, and that the enhanced CNP activity also plays a compensatory role in obstructive uropathy in the glomerulus.

Keywords

References

  1. Ammarguellat F, Larouche I, Schiffrin EL. Myocardial fibrosis in DOCA-salt hypertensive rats: effect of endothelin ET(A) receptor antagonism. Circulation 103: 319-324, 2001 https://doi.org/10.1161/01.CIR.103.2.319
  2. Bakris GL, Re RN. Endothelin modulates angiotensin II-induced mitogenesis of human mesangial cells. Am J Physiol 264: F937- 942, 1993
  3. Barr CS, Rhodes P, Struthers AD. C-type natriuretic peptide. Peptides 17: 1243-1251, 1996 https://doi.org/10.1016/S0196-9781(96)00110-6
  4. Canaan-Kuhl S, Ostendorf T, Zander K, Koch KM, Floege J. C-type natriuretic peptide inhibits mesangial cell proliferation and matrix accumulation in vivo. Kidney Int 53:1143-1151, 1998 https://doi.org/10.1046/j.1523-1755.1998.00895.x
  5. Ferrario CM, Chappell MC. Novel angiotensin peptides. Cell Mol Life Sci 61: 2720-2727, 2004 https://doi.org/10.1007/s00018-004-4243-4
  6. Ferrario CM, Chappell MC, Tallant EA, Brosnihan KB, Diz DI. Counterregulatory actions of angiotensin-(1-7). Hypertension 30: 535-541, 1997 https://doi.org/10.1161/01.HYP.30.3.535
  7. Hsu CH, Kurtz TW, Rosenzweig J, Weller JM. Intrarenal hemodynamics and ureteral pressure during ureteral obstruction. Invest Urol 14: 442-445, 1977
  8. Iyer SN, Chappell MC, Averill DB, Diz DI, Ferrario CM. Vasodepressor actions of angiotensin-(1-7) unmasked during combined treatment with lisinopril and losartan. Hypertension 31: 699- 705, 1998 https://doi.org/10.1161/01.HYP.31.2.699
  9. Ketteler M, Noble NA, Border WA. Transforming growth factorbeta and angiotensin II: the missing link from glomerular hyperfiltration to glomerulosclerosis? Annu Rev Physiol 57: 279-295, 1995 https://doi.org/10.1146/annurev.ph.57.030195.001431
  10. Kim SW, Cho SH, Oh BS, Yeum CH, Choi KC, Ahn KY, Lee J. Diminished renal expression of aquaporin water channels in rats with experimental bilateral ureteral obstruction. J Am Soc Nephrol 12: 2019-2028, 2001a
  11. Kim SW, Lee J, Park JW, Hong JH, Kook H, Choi C, Choi KC. Increased expression of atrial natriuretic peptide in the kidney of rats with bilateral ureteral obstruction. Kidney Int 59: 1274- 1282, 2001b https://doi.org/10.1046/j.1523-1755.2001.0590041274.x
  12. Klahr S. Pathophysiology of obstructive nephropathy: a 1991 update. Semin Nephrol 11: 156-168, 1991
  13. Li N, Zimpelmann J, Cheng K, Wilkins JA, Burns KD. The role of angiotensin converting enzyme 2 in the generation of angiotensin 1-7 by rat proximal tubules. Am J Physiol Renal Physiol 288: F353-F362, 2005 https://doi.org/10.1152/ajprenal.00144.2004
  14. Matsushima H, Yamada N, Matsue H, Shimada S. The effects of endothelin-1 on degranulation, cytokine, and growth factor production by skin-derived mast cells. Eur J Immunol 34: 1910- 1919, 2004 https://doi.org/10.1002/eji.200424912
  15. Motwani JG, McAlpine H, Kennedy N, Struthers AD. Plasma brain natriuretic peptide as an indicator for angiotensin-convertingenzyme inhibition after myocardial infarction. Lancet 341: 1109- 1113, 1993 https://doi.org/10.1016/0140-6736(93)93126-L
  16. Purkerson ML, Klahr S. Prior inhibition of vasoconstrictors normalizes GFR in postobstructed kidneys. Kidney Int 35: 1305- 1314, 1989
  17. Remuzzi G, Bertani T. Pathophysiology of progressive nephropathies. N Engl J Med 339: 1448-1456, 1998 https://doi.org/10.1056/NEJM199811123392007
  18. Reyes AA, Klahr S. Renal function after release of ureteral obstruction: role of endothelin and the renal artery endothelium. Kidney Int 42: 632-638, 1992 https://doi.org/10.1038/ki.1992.328
  19. Ruiz-Ortega M, Egido J. Angiotensin II modulates cell growth-related events and synthesis of matrix proteins in renal interstitial fibroblasts. Kidney Int 52: 1497-1510, 1997 https://doi.org/10.1038/ki.1997.480
  20. Ruiz-Ortega M, Lorenzo O, Suzuki Y, Ruperez M, Egido J. Proinflammatory actions of angiotensins. Curr Opin Nephrol Hypertens 10: 321-329, 2001 https://doi.org/10.1097/00041552-200105000-00005
  21. Shimojo N, Jesmin S, Zaedi S, Maeda S, Soma M, Aonuma K, Yamaguchi I, Miyauchi T. Eicosapentaenoic acid prevents endothelin- 1-induced cardiomyocyte hypertrophy in vitro through the suppression of TGF-beta1 and phosphorylated JNK. Am J Physiol Heart Circ Physiol 291: H835-H845, 2006 https://doi.org/10.1152/ajpheart.01365.2005
  22. Sudoh T, Minamino N, Kangawa K, Matsuo H. C-type natriuretic peptide (CNP): a new member of natriuretic peptide family identified in porcine brain. Biochem Biophys Res Commun 168: 863-870,1990 https://doi.org/10.1016/0006-291X(90)92401-K
  23. Torres VE, Hui YS, Shah SV, Northrup TE, Dousa TP. Cyclic nucleotide phosphodiesterases in glomeruli of rat renal cortex. Kidney Int 14: 444-451, 1978 https://doi.org/10.1038/ki.1978.149
  24. Wolf G. Vasoactive substances as regulators of renal growth. Exp Nephrol 1: 141-151, 1993
  25. Wolf G, Neilson EG. Angiotensin II as a renal growth factor. J Am Soc Nephrol 3: 1531-1540, 1993
  26. Wolf G, Ziyadeh FN, Zahner G, Stahl RA. Angiotensin II is mitogenic for cultured rat glomerular endothelial cells. Hypertension 27: 897-905, 1996 https://doi.org/10.1161/01.HYP.27.4.897
  27. Xue C, Siragy HM. Local renal aldosterone system and its regulation by salt, diabetes, and angiotensin II type 1 receptor. Hypertension 46: 584-590, 2005 https://doi.org/10.1161/01.HYP.0000175814.18550.c0
  28. Yanagisawa H, Morrissey J, Morrison AR, Klahr S. Eicosanoid production by isolated glomeruli of rats with unilateral ureteral obstruction. Kidney Int 37: 1528-1535, 1990 https://doi.org/10.1038/ki.1990.145
  29. Yanagisawa M, Kurihara H, Kimura S, Tomobe Y, Kobayashi M, Mitsui Y, Yazaki Y, Goto K, Masaki T. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332: 411-415, 1988 https://doi.org/10.1038/332411a0
  30. Zhao J, Ardaillou N, Lu CY, Placier S, Pham P, Badre L, Cambar J, Ardaillou R. Characterization of C-type natriuretic peptide receptors in human mesangial cells. Kidney Int 46: 717-725, 1994 https://doi.org/10.1038/ki.1994.326