The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
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Involvement of Caspases and Bcl-2 Family in Nitric Oxide-Induced Apoptosis of Rat PC12 Cells

  • Jeong, Yeon-Jin (Dental Science Research Institute, 2nd Stage of Brain Korea 21, School of Dentistry, Chonnam National University) ;
  • Jung, Ji-Yeon (Dental Science Research Institute, 2nd Stage of Brain Korea 21, School of Dentistry, Chonnam National University) ;
  • Lee, Jin-Ha (Dental Science Research Institute, 2nd Stage of Brain Korea 21, School of Dentistry, Chonnam National University) ;
  • Cho, Jin-Hyoung (Dental Science Research Institute, 2nd Stage of Brain Korea 21, School of Dentistry, Chonnam National University) ;
  • Lee, Guem-Sug (Dental Science Research Institute, 2nd Stage of Brain Korea 21, School of Dentistry, Chonnam National University) ;
  • Kim, Sun-Hun (Dental Science Research Institute, 2nd Stage of Brain Korea 21, School of Dentistry, Chonnam National University) ;
  • Kim, Won-Jae (Dental Science Research Institute, 2nd Stage of Brain Korea 21, School of Dentistry, Chonnam National University)
  • Published : 2006.12.31
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Abstract

This study was aimed to investigate the nitric oxide (NO)-induced cytotoxic mechanism in PC12 cells. Sodium nitroprusside (SNP), an NO donor, decreased the viability of PC12 cells in dose-and time-dependent manners. SNP enhanced the production of reactive oxygen species (ROS), and gave rise to apoptotic morphological changes including cell shrinkage, chromatin condensation, and DNA fragmentation. Expression of Bax was not affected, whereas Bcl-2 was downregulated in SNP-treated PC12 cells. SNP augmented the release of cytochrome c from mitochondria into cytosol and enhanced caspase -8, -9, and -3 activities. SNP upregulated both Fas and Fas-L, which are known to be components of death receptor assembly. These results suggest that NO induces apoptosis of PC12 cells through both mitochondria-and death receptor-mediated pathways mediated by ROS and Bcl-2 family.

Keywords

References

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