Applied Biological Chemistry

  • 제49권4호
  • /
  • Pages.334-338
  • /
  • 2006
  • /
  • 2468-0834(pISSN)
  • /
  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

장수풍뎅이 유충으로부터 지표성분(Inosine)의 분리 및 정량분석

Isolation and Quantitative Analysis of Inosine as an Index Component from Larva of Allomirina dichotoma

  • 이진희 (경희대학교 생명과학대학) ;
  • 송명종 (경희대학교 생명과학대학) ;
  • 양혜정 (경희대학교 생명과학대학) ;
  • 방면호 (강화군 특화작목연구소) ;
  • 정동준 (경희대학교 생명과학대학) ;
  • 김영채 (경희대학교 생명과학대학) ;
  • 김세영 (경희대학교 생명과학대학) ;
  • 노영덕 (경희대학교 생명과학대학) ;
  • 정성현 (경희대학교 약학대학) ;
  • 백남인 (경희대학교 생명과학대학)
  • 발행 : 2006.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

장수풍뎅이 유충의 MeOH추출물에서 얻어진 n-BuOH 분획으로부터 silica gel column chromatography를 반복하여 핵산성분을 분리하였으며, NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여 inosine으로 동정하였다. Inosine을 지표성분으로 하여 $NH_2$-column을 이용한 HPLC 분석방법을 확립하였고, 열풍건조한 시료와 원적외선 건조기로 건조한 장수풍뎅이 유충 시료 중의 inosin의 함량을 각각 0.003% 및 0.055%로 정량분석하였다.

A repeated silica Rel column chromatography of n-BuOH fraction from the larva of Allomirina dichotoma afforded a purified nucleic acid, which was identified as inosine on the basis of the spectroscopic data including IR, MS and NMR. The HPLC analysis of the index component using silica based $NH_2$-column exhibited the contents in the hot air-dried and Far-IR-dried larvae of Allomirina dichotoma as 0.003% and 0.055%, respectively.

키워드

참고문헌

  1. Li, S. (1998) In Ben Cao Gang Mu, Compendium of Materia Medica. Renmin Weisheng Chubanshe, Peking
  2. Heo, J. (2005) In Dongeui Bogam (3rd ed.). YeoGang Press, Seoul
  3. Korea Food & Drug Administration (2002) In The Korean Herbal Pharmacopoeia. Dongwon-Moonwhasa, Seoul
  4. Jiang, S. X. and Yi, X. Y. (1982) In Dictionary of Chinese Materia Mediaca. Shanghai Scientific & Technical Publishers, Shanghai
  5. Shin, M. K. (1997) In Clinical Traditional Herbalogy (5th ed.). Younglimsa, Seoul
  6. Kang, I. J., Kim, H. K., Chung, C. K., Kim, S. J. and Oh, D. H. (2000) Effect of Protaetia orientalis (Groy et Perchlon) Larva on the lipid metabolism in ethanol administered rats. J. Korean Soc. Food Sci. Nutr. 29, 479-484
  7. Kang, I. J., Chung, C. K., Kim, S. J., Nam, S. M. and Oh, S. H. (2001) Effect of Protaetia orientalis (Groy et Perchlon) larva on the lipid metabolism in carbon tetrachloride administered rats. Korean J. Soc. Electron Microscopy 31, 9-18
  8. Ahn, M. Y., Ryu, K. S., Lee, Y. W. and Kim, Y. S. (2000) Cytotoxicity and L-amino acid oxidase activity of crude insect drugs. Arch. Pharm. Res. 23, 477-481 https://doi.org/10.1007/BF02976576
  9. The Korean Pharmaceutical Association & Herb Medicine Policy Committee (2001) In (WeonSaeg) HanYagDoGam. Academy Press, Seoul
  10. Hong, J. O. (1994) In Gagongsigpumwi Weonryoro Sayonghal Su Itneun Dongsigmule Gwanhan Yeongu. Korea Advanced Food Reseach Institute, Seoul
  11. Rodrigues, M. C. A., Guizzo, R., Gobbo-Neto, L., Ward, R. J., Lopes, N. P. and Ferreira dos Santos, W. (2004) The biological activity in mammals and insects of the nucleosidic fraction from the spider Parawixia bistriata. Toxicon. 43, 375-383 https://doi.org/10.1016/j.toxicon.2004.01.009
  12. Gao, J. M., Shen, J., Zhang, A. L., Zhu, W., Zhang, X, and Liu, J. K. (2003) Chemical constituents of the fungus Leccinum extremiorientale. Youji Huaxue 23, 853-857
  13. Lidgren, G., Bohlin, L. and Christophersen, C. (1988) Studies of Swedish marine organisms. Part X. Biologically active compounds from the marine sponge Geodia baretti. J. Nat. Prod. 51, 1277-1280 https://doi.org/10.1021/np50060a041
  14. Starling, R. D., Trappe, T. A. and Short, K. R. (1996) Effect of inosine supplementation on aerobic and anaerobic cycling performance. Med. Sci. Sports Ex. 28, 1193-1198 https://doi.org/10.1097/00005768-199609000-00017
  15. Yao, Q. and Gao, G. (2005) Effects of inosine on praxiology and the level of dopamine in striatum of mice with Parkinson disease. Zhongguo Linchuang Kangfu 9, 55-57
  16. Devous, M. D. and Lewandowski, E. D. (1987) Inosine preserves ATP during ischemia and enhances recovery during reperfusion. Am. J. Physiol. 253, 1224-1233
  17. Macdonald, G. J., Burcher, E., Fisher, W. K., Bagnara, A. S., Barrow, K. D., Thompson, E. O. P. and Duffield, A. M. (1981) Uridine and inosine: pressor nucleosides from man, rat and dog. Aust. J. Exp. Biol. and Med. Sci. 59, 167-78 https://doi.org/10.1038/icb.1981.12
  18. Lewandowski, E. D., Johnston, D. L. and Roberts, R. (1991) Effects of inosine on glycolysis and contracture during myocardial ischemia. Circul. Res. 68, 578-87 https://doi.org/10.1161/01.RES.68.2.578
  19. Darlington, D. N. and Gann, D. S. (2005) Purine nucleosides stimulate Na/K ATPase, and prolong survival in hemorrhagic shock. J. Trauma: Injury, Infection, and Critical Care 58, 1055-1060 https://doi.org/10.1097/01.TA.0000169801.08019.B9
  20. Tomaselli, B., Podhraski, V., Heftberger, V., Boeck, G. and Baier-Bitterlich, G. (2005) Purine nucleoside-mediated protection of chemical hypoxia-induced neuronal injuries involves p42/44 MAPK activation. Neurochem. International 46, 513-521 https://doi.org/10.1016/j.neuint.2005.02.003