Effect of Mepanipyrim on the Resistant Isolates of Gray Mold fungus, Botrytis Cinerea to the Fungicides of Benzimidazole and Dicarboximide

Benzimidazole과 dicarboximide계 살균제 저항성 잿빛곰팡이병원균(Botrytis cinerea)에 대한 mepanipyrim의 효과

  • Koo, Han-Mo (Department of Plant Resources, Kongju National University) ;
  • An, Seung-Joon (Department of Plant Resources, Kongju National University) ;
  • Shin, Ho-Chul (Central Research Institute of Kyung Nong Cooperation) ;
  • Do, Eun-Soo (Department of Oriental Medicine Resources, Jungbu National University) ;
  • Shin, Mi-Ho (Department of Crop Science, Konkuk University) ;
  • Kim, You-Seok (Department of Crop Science, Konkuk University) ;
  • Kim, Jin-Hee (Department of Crop Science, Konkuk University) ;
  • Chun, Se-Chul (Department of Molecular Biotechnology, Konkuk University)
  • 구한모 (공주대학교 식물자원학과) ;
  • 안승준 (공주대학교 식물자원학과) ;
  • 신호철 (경농(주) 중앙연구소) ;
  • 도은수 (중부대학교 한약자원학과) ;
  • 신미호 (건국대학교 식량자원학과) ;
  • 김유석 (건국대학교 식량자원학과) ;
  • 김진희 (건국대학교 식량자원학과) ;
  • 천세철 (건국대학교 분자생명공학과)
  • Published : 2006.12.31

Abstract

Effect of the fungicide mepanipyrim on the resistant and sensitive isolates of Botrytis cinerea was studied in vitro and also tested to control Botrytis rot of strawberry, cucumber and grape in the field. These isolates were selected by relative mycelial growth and spore germination on potato dextrose agar(PDA) incorporated with $100{\mu}g\;a.i./ml$ of benomyl and procymidone, respectively, compared to the unamended PDA. Mycelial growth of the selected resistant isolates was significantly inhibited by mepanipyrim but the inhibition rate was similar to other fungicides belong to benzimidazole or dicarboximide, although spore germination was not inhibited even by the higher concentration of mepanipyrim. When the benomyl and procymidone resistant isolates were inoculated to cucumber leaves, lesion development was significantly inhibited with application of $250{\mu}g\;a.i./ml$ of mepanipyrim but not with that of benomyl and procymidone. In addition, when $250{\mu}g\;a.i./ml$ of mepanipyrim was applied to strawberry, cucumber, and grape in the field, the control of Botrytis rot was significantly different from that of the untreated control(Duncan's multiple range test, p<0.05). The results suggested that mepanipyrim might be an alternative fungicide for the control of benomyl- and procymidone-resistant pathogens of Botrytis rot.

Benomyl과 procymidone에 대한 잿빛곰팡이병원균(Botrytis cinerea) 저항성 균주와 감수성 균주에 대한 mepanipyrim의 효과를 실내에서 조사하고 딸기, 오이, 포도에 대한 포장에서의 잿빛곰팡이병의 방제 효과를 시험하였다. 잿빛곰팡이병원균(Botrytis cinerea)의 균사 생장과 포자 발아율의 비교를 기초로 하여 benomyl과 procymidone을 각각 $100{\mu}g\;a.i./ml$씩 첨가한 감자한천배지(potato dextrose agar, PDA)에서 benomyl과 procymidone 살균제에 저항성 또는 감수성인 균주를 선발하였다. 선발된저항성 균들의 포자발아는 mepanipyrim의 농도가 증가함에도 불구하고 현저히 억제되지는 않았다. 그러나 포자 발아와는 대조적으로 균사의 생장은 benzimidazole 또는 dicarboximide계의 기존 다른 약제와 유사하게 억제되었다. Benomyl과 procymidone에 저항성인 분리 균주를 오이 잎에 접종한 후 mepanipyrim $250{\mu}g\;a.i./ml$을 처리하였을 때 병원균의 침투력이 현저히 억제되었지만 benomyl과 procymidone을 처리하였을 때는 그렇지 못했다. Mepanipyrim $250{\mu}g\;a.i./ml$을 포장에서 딸기, 오이, 포도에 처리하였을 때 잿빛곰팡이병이 현저히 억제된 것으로 나타나(Duncan's multiple range test, p<0.05), mepanipyrim이 benzimidazole과 dicarboximide계 등의 다른 살균제에 저항성인 잿빛곰팡이병원균에 의한 병을 방제하는데 효과적으로 사용될 수 있음을 제시하여 주었다.

Keywords

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