Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Auxin Induced Expression of Expansin is Alered in a New Aux1 Allele that Shows Severe Defect in Gravitropic Response

  • Jeong, Hae-Jun (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Kwon, Ye-Rim (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Oh, Jee-Eun (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Ki-Deok (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University) ;
  • Lee, Sung-Joon (Department of Food Science, College of Life and Environmental Sciences, Korea University) ;
  • Hong, Suk-Whan (Division of Applied Plant Science, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Lee, Ho-Joung (Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University)
  • Published : 2006.12.31
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Abstract

While the underlying molecular mechanism remains to be elucidated, recent studies suggest that polar auxin transport is a key controlling factor in triggering differential growth responses to gravity. Identification of regulatory components in auxin-mediated differential cell expansion would improve our understanding of the gravitropic response. In this study, we identify a mutant designated aux1-like(later changed to aux1), an allele of the aux1 mutant that exhibits a severely disrupted root gravitropic response, but no defects in developmental processes. In Arabidopsis, AUX1 encodes an auxin influx carrier. Since in-depth characterization of the gravitropic response caused by mutations in this gene has been performed previously, we focused on identifying the downstream genes that were differentially expressed compared to wild-type plants. Consistent with the mutant phenotype, the transcription of the auxin-responsive genes IAA17 and GH3 were altered in aux1 plants treated with IAA, 2, 4-D and NAA. In addition, we identified two expansin genes EXP10 and EXPL3 that exhibited different expression in wild-type and mutant plants.

Keywords

References

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