한국초지조사료학회지 (Journal of The Korean Society of Grassland and Forage Science)

  • 제26권4호
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  • Pages.267-276
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  • 2006
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  • 2287-5824(pISSN)
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  • 2287-5832(eISSN)
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
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월동기간 중 Zoysiagrass와 Creeping Bentgrass의 저온내성에 대한 항산화 효소 및 지질과산화의 비교 연구

Comparative Study on Antioxidant Enzymes and Lipid Peroxidation Related Low Temperature Tolerance in Overwintering Zoysiagrass and Creeping Bentgrass

  • Kim, Dae-Hyun (Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University) ;
  • Lee, Bok-Rye (Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University) ;
  • Lee, Jae-Sik (Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University) ;
  • Li, Ming (Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University) ;
  • Kim, Tae-Hwan (Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University)
  • 발행 : 2006.12.30
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초록

월동기간 동안 zoysiauass [Zoysia matrella (L.) Merr]와 creeping bentgrass(Agrostis palustris Hunds)의 뿌리에서 저온 스트레스에 대한 생리학적 반응을 규명하기 위해 지질과산화 정도와 효소적 항산화 반응 및 탄수화물의 변화를 조사하였다. 1월 말 Creeping bentgrass의 뿌리 고사율은 zoysiagrass 보다 약 20% 현저히 높게 나타났다. Creeping bentgrass의 뿌리 성장은 온도변화와 거의 평행하게 변화하였으나 zoysiauass는 뿌리 성장에 거의 변화가 없이 4월 말까지 유지되었다. Polyssacharide(starch+fructan)의 함량은 두 품종에서 별다른 차이는 발견되지 않았으나 zoysiagrass의 총비구조적 탄수화물이 creeping bentgrass에 비해 약 10% 높게 나타났다. Malondialdehyde(MDA)의 농도는 creeping bentgrass에서 현저히 높게 나타났다. Creeping bentgrass의 peroxidase의 활성은 zoysiagrass에 비해 약 4.2 배 높았으나 superoxide(SOD)와 catalase(CAT)의 활성은 각각 22%와 67% 낮았다. 이상의 결과들은 creeping bentgrass 보다 zoysiagrass가 월동 기간 중 저온 내성 메커니즘을 우선적으로 작동시키며 저온 스트레스에 대해 덜 민감하다는 것을 잘 나타내고 있다.

To investigate the physiological responses to winter freezing stress naturally occurring, the level of lipid peroxidation and enzymatic antioxidant responses were compared between zoysiagrass and creeping bentgrass during overwintering. Root mortality of creeping bentgrass was significantly higher than zoysiagrass at January. Root growth of creeping bentgrass was nearly parallel with temperature fluctuation, while zoysiagrass showed little changes in root growth until the end of April. Total nonstructural carbohydrate of zoysiauass was 10% higher than creeping bentgrass. Malondialdehyde(MDA) content in creeping bentgrass was 2-fold higher than that of zoysiagrass. The peroxidase(POD) activity of creeping bentgrass in January was 4.2 times higher, while superoxide(SOD) and catalase(CAT) activities lowered 22% and 67%, respectively, compared to zoysiagrass. These results suggest that zoysiagrass roots much properly operate cold tolerance mechanism and: are less susceptible to cold stress in comparison to creeping bentgrass.

키워드

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