Journal of Biosystems Engineering

  • 제31권6호
  • /
  • Pages.535-540
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  • 2006
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  • 1738-1266(pISSN)
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  • 2234-1862(eISSN)
한국농업기계학회 (Korean Society for Agricultural Machinery)
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살균제 Iprovalicarb 잔류물의 신속한 검출을 위한 효소면역분석법

Enzyme Immunoassay for Rapid Detection of the Fungicide Iprovalicarb Residues

  • Cho, H.K. (Dept. of Biosystems Engineering, Chungbuk National University) ;
  • Kyung, K.S. (Dept. of Agricultural Chemistry, Chungbuk National University) ;
  • Lee, E.Y. (Dept. of Agricultural Chemistry, Chungbuk National University)
  • 발행 : 2006.12.25
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초록

For a biosensor development, an enzyme-linked immunosorbent assay (ELISA) of the fungicide iprovalicarb was developed by minimizing the processing time. The time for whole incubation process was reduced from 135 minutes to 15 minutes. The concentration of antibody was varied to improve sensitivity. The total processing time was reduced from 2.5 hours to 20 minutes, the final sensitivity ($IC_{50}$ value) of 7.93 ng/mL and the lowest detection limit of 0.045 ng/mL were obtained. This ELISA was applied to potatoes and onions, and the recoveries were in the range of 98.85 $\sim$ 101.20% and 87.97 $\sim$ 102.70%, respectively. Accordingly, this method can be used as basis for a biosensor for rapid monitoring of iprovalicarb residues in crops.

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참고문헌

  1. Lee, J. K., S. H. Park, E. Y. Lee, Y. J. Kim and K. S. Kyung. 2004. Development of an ELISA for the detection of the residues of the fungicide Iprovalicarb. J. Agric. Food Chem. 52:6680-6686 https://doi.org/10.1021/jf048840a
  2. Rodbard, D. 1981. Mathematics and statics of ligand assay: an illustrated guide. In Ligand Assay: Analysis of International Developments on Isotopic and Nonisotopic Immunoassay; Langan, J., Clapp, J. J., Eds., Masson Publishing Co., New York, 45-99
  3. Tomlin, C. D. S. 2003. A world compendium : The pesticide manual. 13th ed. British crop protection council publications. UK. pp.580
  4. 박선화. 2003. 살균제 Fenarimol 잔류물의 효소면역분석법 개발. 충북대학교 석사학위논문
  5. 송석진, 조한근. 2003. 살충제 Imidacloprid 잔류물의 실시간 측정용 효소면역분석법 개발. 한국농업기계학회지 28(6):505-510
  6. 식품의약품안전청. 2005. 식품의 농약 잔류허용기준(MRLs for pesticides in foods). pp.112
  7. 이은영. 2005. 다클론 항체를 이용한 살균제 Iprovalicarb의 효소면역분석법 개발. 충북대학교 석사학위논문
  8. 한국작물보호협회. 2006. 농약사용지침서. pp.203

피인용 문헌

  1. A Biosensor to Measure Fungicide Iprovalicarb Residues in Agricultural Products vol.1, pp.2, 2008, https://doi.org/10.1016/S1881-8366(08)80004-3