Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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High-yield Production of Functional Human Lactoferrin in Transgenic Cell Cultures of Siberian Ginseng(Acanthopanax senticosus)

  • Jo, Seung-Hyun (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Kwon, Suk-Yoon (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Park, Doo-Sang (Insect Resources Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Yang, Kyoung-Sil (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Kim, Jae-Whune (Microplants Co., Ltd.) ;
  • Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University) ;
  • Kwak, Sang-Soo (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Lee, Haeng-Soon (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
  • Published : 2006.10.30
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Abstract

Human lactoferrin (hLf) is an iron-binding glycoprotein that has been considered to play many biological roles in the human, including the stimulation of the immune system, antimicrobial and anti-inflammatory effects, and regulation of iron absorption. We generated transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing a functional hLf protein using the signal peptide sequence from the endoplasmic reticulum and driven by an oxidative stress-inducible SWPA2 promoter which is highly expressed in plant cell cultures. The production of hLf increased proportionally to cell growth and showed a maximal level (up to 3.6% of total soluble protein) at the stationary phase in suspension cultures. Full-length hLf protein was identified by immunoblot analysis in transgenic cell cultures of Siberian ginseng. Recombinant hLf (rhLf) was purified from suspension cells of Siberian ginseng by ammonium sulfate precipitation, cation-exchange and gel filtration chromatography. N-terminal sequences of rhLf were identical to native hLf (nhLf). The overall monosaccharide composition of rhLf showed the presence of plant specific xylose while sialic acid is absent. Antibacterial activity of purified rhLf was higher than that of nhLf. Taken together, we anticipate that medicinal Siberian ginseng cultured cells, as demonstrated by this study, will be a biotechnologically useful source for commercial production of functional hLf not requiring further purification.

Keywords

References

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