The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Antibacterial Activities against Plant Pathogens and Identification of Agrimol B from Agrimonia pilosa LEDEB

식물병원균에 대한 짚신나물 (선학초) 추출물의 항균활성과 Agrimol B의 동정

  • Chun, Sung-Bong (Korea Minjok Leadership Academy, Joongbu University the Analytical Center of Life Science) ;
  • Yang, Ba-Rom (Korea Minjok Leadership Academy, Joongbu University the Analytical Center of Life Science) ;
  • Choi, Chun-Whan (Korea and Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Kim, Ik-Soo (Korea and Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Park, Kyung-Seok (National Institute of Agricultural Science and Technology, RDA)
  • 전성봉 (민족사관고등학교) ;
  • 양바롬 (민족사관고등학교) ;
  • 최춘환 (중부대학교 산학협력단 생명과학분석센터) ;
  • 김익수 (중부대학교 산학협력단 생명과학분석센터) ;
  • 박경석 (농촌진흥청 농업과학기술원 식물병리과 생물제어연구실)
  • Published : 2006.09.30
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Abstract

Eighty-five percent methanol extract of Agrimonia pilosa has antibacterial activity against Pseudomonas syringae pv. lacrymans (bacterial leaf spot pathogen), Ralstonia solanacearum (tomato bacterial wilt pathogen) and Pseudomonas syringae pv. tabaci (Tobacco wild fire pathogen.). The active substance was purified by silica gel column chromatography and HPLC. The molecular weight of the active compound was determined by LC-Mass as 687.2. With NMR analysis, the active substance was identified as Agrimol B.

선학초 (전초) 메탄올 조추출물이 오이 세균성모무늬병균인 Pseudomonas syringae pv. lacrymans, 토마토 풋마름병균인 Ralstonia solanacearum 및 담배세균성들불병균인 Pseudomonas syringae pv. tabaci에 대해서 항균활성을 나타내었다. 활성성분을 silica gel column 및 HPLC로 순수분리하여 LC-Mass 분석 결과 분자량 682.7을 갖는 물질로 추정 할 수 있었으며, 선학초에서 분리된 물질중 분자량 687.2를 나타내는 Agrimol A와 B를 구별하기 위하여 $^1H-NMR$, $^{13}C-NMR$ 스팩트럼 분석 결과 항균활성성분이 Agrimol B임을 확증할 수 있었다.

Keywords

References

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