Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Bactericidal Activity of Grapefruit (Citrus paradisl) Seed Extract-Based Disinfectant

  • Han, Jae-Hong (Metalloenzyme Research Group, BET Research Institute and Department of Biotechnology, Chung-Ang University) ;
  • Kim, Yong-Ung (Metalloenzyme Research Group, BET Research Institute and Department of Biotechnology, Chung-Ang University) ;
  • Kim, Ki-Yeon (Metalloenzyme Research Group, BET Research Institute and Department of Biotechnology, Chung-Ang University) ;
  • Hahm, Young-Tae (Metalloenzyme Research Group, BET Research Institute and Department of Biotechnology, Chung-Ang University)
  • Published : 2006.09.30
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Abstract

Bactericidal activity of grapefruit seed extract (GSE)-based disinfectant, as a safe disinfectant, was measured against five bacteria by Korean Food & Drug Administration (KFDA) dilution-neutralization method. GSE-based disinfectant showed a 99.9999% bactericidal activity against Escherichia coli ATCC 10536, Salmonella typhi ATCC 29629, Staphylococcus aureus ATCC 6538, Bacillus cereus ATCC 11778, and Listeria monocytogenes ATCC 1911 at the concentration of 2.15% GSE. It showed better bactericidal activity against Gram-negative bacteria of E. coli ATCC 10536 and S. typhi ATCC 29629 at lower concentration of GSE (0.43%). Based on the results, it was suggested that a possible bactericidal mechanism of GSE active ingredients was due to the abrupt osmotic shift during the bactericidal activity test by KFDA method.

Keywords

References

  1. Korean Food & Drug Administration Notice 2005-75
  2. Park, W.-P. and Chang, D.-K. (2003) Kimchi quality affected by the addition of grapefruit seed extract powder. Kor. J. Food Pres. 10, 288-292
  3. Kim, H.-S., Jung, S.-K., Cho, S.-H., Ku, J.-G. and Lee, S.- C. (2003) Preparation and effect of eudragit E100 microcapsules containing grapefruit seed extract on Kimchi. J. Kor. Soc. Food Sci. Nutr. 32, 1239-1244 https://doi.org/10.3746/jkfn.2003.32.8.1239
  4. Armando, C., Maythe, S. and Beatriz, N. P. (1998) Antioxidant activity of grapefruit seed extract on vegetable oils. J. Sci. Food. Agric. 77, 463-467 https://doi.org/10.1002/(SICI)1097-0010(199808)77:4<463::AID-JSFA62>3.0.CO;2-1
  5. Cho, S.-H., Kim, C.-H. and Park, W.-P. (2004) Antimicrobial activities of botanical antimicrobial agent-grapefruit seed extract mixture for the preparation of antimicrobial packaging paper. Kor. J. Food Pres. 11, 411-416
  6. Reagor, L., Gusman, J., McCoy, L., Carino, E. and Heggers, J. P. (2002) The effectiveness of processed grapefruitseed extract as an antibacterial agent: I. An in vitro agar assay. J. Altern. Compl. Med. 8, 325-332 https://doi.org/10.1089/10755530260128014
  7. Heggers, J. P., Cottingham, J., Gusman, J., Reagor, L, McCoy, L., Carino, E., Cox, R. and Zhao, J.-G. (2002) The effectiveness of processed grapefruit-seed extract as an antibacterial agent: II. Mechanism of action and in vitro toxicity. J. Altern. Compl. Med. 8, 333-340 https://doi.org/10.1089/10755530260128023
  8. Cvetniæ, Z. and Vladimir-Knezeviae, S. (2004) Antimicrobial activity of grapefruit seed and pulp ethanolic extract. Acta Pharm. 54, 243-250
  9. Brock, T. D. and Madigan, M. T. (1988) Measurement of growth, In Biology of microorganisms (5th ed.) pp. 317- 320, Prentice Hall, New Jersey
  10. KFDA notice 2005-33. Dilution-neutralization method. www.kfda.go.kr
  11. Basile, A., Sorbo, S., Giodano, S., Ricciardi, L., Ferrara, S., Montesano, D., Castaldo Cobianchi, R., Vuotto, M. L. and Ferrara, L. (2000) Antibacterial and alleopathic activity of extract from Castanea sativa leaves. Fitoterapia 71, S110- S116 https://doi.org/10.1016/S0367-326X(00)00185-4
  12. Sakamoto, S., Sato, K., Maitani, T. and Yamada, T. (1996) Analysis of components in natural food additive grapefruit seed extract by HPLC and LC/MS. Eisei Shikenjo Hokoku. 114, 38-42
  13. von Woedtke T., Schluter, B., Pflegel, P., Lindequist, U. and Julich, W. D. (1999) Aspects of the antimicrobial efficacy of grapefruit seed extract and its relation to preservative substances contained. Pharmazie 54, 452-456
  14. Takeoka, G. R., Dao, L. T., Wong, R. Y. and Harden, L. A. (2005) Identification of benzalkonium chloride in commercial grapefruit seed extract. J. Agric. Food Chem. 53, 7630- 7636 https://doi.org/10.1021/jf0514064
  15. Aarestrup, F. M. and Hasman, H. (2004) Susceptibility of different bacterial species isolated from food animals to copper sulphate, zinc chloride and antimicrobial substances used for disinfection. Vet. Microbiol. 100, 83-89 https://doi.org/10.1016/j.vetmic.2004.01.013
  16. Schweizer, H. P. (2001) Triclosan: a widely used biocide and its link to antibiotics. FEMS Microbiol. Lett. 202, 1-7 https://doi.org/10.1111/j.1574-6968.2001.tb10772.x
  17. Mellefont, L. A., McMeekin, T. A. and Ross, T. (2003) The effect of abrupt osmotic shifts on the lag phase duration of foodborne bacteria. Int. J. Food Microbiol. 83, 281-293 https://doi.org/10.1016/S0168-1605(02)00377-X