Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Genetic Toxicity Test of Glycidol by Ames, Micronucleus, Comet Assays and Microarray Analysis

  • Kim, Ji-H. (College of Pharmacy, Ewha Womans University) ;
  • Kim, Ki-Y. (College of Pharmacy, Ewha Womans University) ;
  • Kwon, Kyoung-J. (College of Pharmacy, Ewha Womans University) ;
  • Go, Seo-Y. (College of Pharmacy, Ewha Womans University) ;
  • Min, Kyung-N. (College of Pharmacy, Ewha Womans University) ;
  • Lee, Woo-S. (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Park, Sue-N. (Department of Toxicological Researches, National Institute of Toxicological Research, Korea Food and Drug Administration) ;
  • Sheen, Yhun-Y. (College of Pharmacy, Ewha Womans University)
  • Published : 2006.12.30
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Abstract

The primary use for glycidol is as a stabilizer in the manufacture of vinylpolymers, however, it is also used as an intermediate in the production of pharmaceuticals, as an additives for oil and synthetic hydraulic fluids, and as a diluting agent is same epoxy resins. In this study, we have carried out in vitro genetic toxicity test of glycidol and microarray analysis of differentially expressed genes in response to glycidol. The result of Ames test showed mutations with glycidol treatment in base substitution strain TA1535 both with and without exogenous metabolic activation. Likewise, glycidol showed mutations in frame shift TA98 both with and without exogenous metabolic activation. The result of COMET assay in L5178Y cells with glycidol treatment showed DNA damage both with and without exogenous metabolic activation. Glycidol increased micronuclei in CHO cells both with and without exogenous metabolic activation. 150 Genes were selected as differentially expressed genes in response to glycidol by microarray analysis and these genes would be candidate biomarkers of genetic toxic action of glycidol.

Keywords

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