Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
권호 표지

Effects of the Constituents of Paeonia lactiflora Root on Arachidonate and NO Metabolism

  • Choi, Yong-Hwan (College of Pharmacy, Kangwon National University) ;
  • Gu, Lianyu (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Yeong-Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kang, Sam-Sik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Ju-Sun (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Yean, Min-Hye (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Hyun-Pyo (College of Pharmacy, Kangwon National University)
  • Published : 2006.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

In order to establish the anti-inflammatory cellular mechanism of the paeony root(Paeonia lactiflora, Pall, Paeoniaceae), the constituents including paeoniflorin, albiflorin, (+)-catechin, paeonol, benzoic acid and methyl gallate were evaluated for their effects on arachidonate and NO metabolism. Among the compounds tested, only paeonol weakly inhibited cyclooxygenase-2-mediated $PGE_2$ production from LPS-treated RAW 264.7 cells. (+)-Catechin and methyl gallate weakly inhibited inducible nitric oxide synthase-mediated NO production from the same cell line. In particular, methyl gallate significantly inhibited 5-lipoxygenase from RBL-l cells with an $IC_{50}$ of 8.4 ${\mu}M$. These results suggest that the inhibition of these components on arachidonate and NO metabolism may contribute at least in part to anti-inflammatory mechanism of the paeony root.

Keywords

References

  1. Chi, Y. S., Cheon, B. S. and Kim, H. P. (2001). Effect of wogonin, a plant flavone from Scutellaria radix, on the suppression of cydooxygenase-2 and the induction of inducible nitric oxide synthase in lipopolysaccharide-treated RAW 264.7 cells. Biochem. Pharmacol. 61, 1195-1203 https://doi.org/10.1016/S0006-2952(01)00597-4
  2. Gallin, J. I. and Snyderman, R. (1999). Overview. In Inflammation: Basic principles and clinical correlates 3rd ed.(Gallin, J. I. and Snyderman, R., Eds.), pp. 1-4. Lippincott Williams & Wilkins, Philadelphia
  3. Huh, Z. (1966). In Dong-Eu-Bo-Gam, Translated Ed., pp. 1160. Namsandang, Seoul
  4. Kang, S. S., Kim, J. S., Yun-Choi, H. S. and Han, B. H. (1993). Phytochemical Studies on Paeoniae Radix. Kor. J. Pharmacogn. 24, 247-250
  5. Kim, S. J., Jin, M., Lee, E., Moon, T. C., Quan, Z., Yang, J. H., Son, K. H., Kim, K-U., Son, J. K. and Chang, H. W. (2006). Effects of methyl gallate on arachidonic acid metabolizing enzymes: Cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells. Arch. Pharm. Res. 29, 874-878 https://doi.org/10.1007/BF02973908
  6. Lee, S. C., Kwon, Y. S., Son, K. H., Kim, H. P. and Heo, M. Y. (2005). Antioxidative constituents from Paeonia lactiflora. Arch. Pharm. Res. 28, 775-783 https://doi.org/10.1007/BF02977342
  7. Lee, S. J., Lee, I. S. and Mar, W. (2003). Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 activity by 1,2,3, 4,6-penta-O-galloyl-B-D-glucose in murine macrophage cells. Arch. Pharm. Res. 26, 832-839 https://doi.org/10.1007/BF02980029
  8. Leem, K., Kim, H., Boo, Y., Lee, H. S., Kim, J. S., Yoo, Y. C., Ahn, H. J., Park, H. J., Seo, J. C., Kim, H. K., Jin, S. Y., Park, H. K., Chung, J. H. and Cho, J. J. (2004). Effects of Paeonia lactiflora extracts on the secretions of monocyte chemotactic protein-1 and -3 in human nasal fibroblasts. Phytother. Res. 18, 241-243 https://doi.org/10.1002/ptr.1392
  9. Lin, H. C., Ding, H. Y. and Ko, F. N. (1999). Aggregation inhibitory activity of minor acetophenones from Paeonia species. Planta Med. 65, 595-599 https://doi.org/10.1055/s-1999-14030
  10. Mossman T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxic assay. J. Immunol. Methods 65, 55-63 https://doi.org/10.1016/0022-1759(83)90303-4
  11. Ohta, H., Ni, J. W. and Matsumoto, K. (1993). Paeony and its major constituent, paeoniflorin, improve radial maze performance impaired by scopolamine in rats. Pharmacol. Biochem. Behav. 45, 719-723 https://doi.org/10.1016/0091-3057(93)90530-7
  12. Prieto, J. M., Recio, M. C., Giner, R. M., Manez, S., Giner-Larza, E. M. and Rios, J. L. (2003). Influence of traditional Chinese anti-inflammatory medicinal plants on leukocyte and platelet functions. J. Pharm. Pharmacol. 55, 1275-1282 https://doi.org/10.1211/0022357021620
  13. Qi, X. G. (1991). Protective mechanism of Salvia miltiorrhiza and Paeonia lactiflora for experimental liver damage. Chung His I Chieh Ho Tsa Chih 11, 102-104
  14. Takagi, K. and Harada, M. (1969). Pharmacological studies on herb paeony root. II. Anti-inflammatory effect, inhibitory effect on gastric juice secretion, preventive effect on stress ulcer, antidiuretic effect of paeoniflorin and combined effects with licorice component Fm 100. Yakugaku Zasshi 89, 887-892 https://doi.org/10.1248/yakushi1947.89.7_887
  15. Tries, S., Neupert, W. and Laufer, S. (2002). The mechanism of action of the new anti-inflammatory compound ML3000: inhibition of 5-LOX and COX-1/2. Inflamm. Res. 51, 135-143 https://doi.org/10.1007/PL00000285
  16. Wu, H. K. and Sheu, S. J. (1996). Capillary electrophoretic determination of the constituents of Paeoniae Radix. J. Chromatogr. A 753, 139-146 https://doi.org/10.1016/S0021-9673(96)00525-0
  17. Yamahara, J., Yamada, T., Kimura, H., Sawada, T. and Fujimura, H. (1982). Biologically active principles of crude drugs. II. Antiallergic principles in 'Shoseiryu- To' anti-inflammatory properties of paeoniflorin and its derivatives. J. Pharmacobiodyn. 5, 921-929 https://doi.org/10.1248/bpb1978.5.921