Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Phellopterin Suppresses Airway Mucin Secretion induced by Adenosine Triphosphate

  • Heo, Ho-Jin (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Kim, Cheol-Su (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Lee, Hyun-Jae (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Kim, Jin-Woong (Department of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Kim, Young-Sik (Department of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Kang, Sam-Sik (Department of Pharmaceutical Sciences, College of Pharmacy, Seoul National University) ;
  • Seo, Un-Kyo (Department of Oriental Medicine, College of Oriental Medicine, Dongguk University) ;
  • Kim, Yun-Hee (Department of Oriental Medicine, College of Oriental Medicine, Daejeon University) ;
  • Park, Yang-Chun (Department of Oriental Medicine, College of Oriental Medicine, Daejeon University) ;
  • Seok, JeongHo (Department of Pharmacology, College of Medicine, Chungnam National University) ;
  • Lee, Choong-Jae (Department of Pharmacology, College of Medicine, Chungnam National University)
  • Published : 2006.12.30
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Abstract

Angelicae Dahiricae Radix has been used for controlling inflammatory respiratory diseases in oriental medicine and their components, phellopterin, isoimperatorin and byakangelicol were reported to have various biological effects. In this study, we investigated whether phellopterin, isoimperatorin and byakangelicol affect adenosine triphosphate(ATP)-induced mucin secretion from cultured airway epithelial cells. Confluent primary hamster tracheal surface epithelial(HTSE) cells were metabolically radiolabeled using $^3H$-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on $^3H$-mucin secretion. The results were as follows: 1) phellopterin significantly inhibited ATP-induced mucin secretion; 2) However, isoimperatorin and byakangelicol did not affect ATP-induced mucin secretion, significantly. This result suggests that phellopterin can regulate 'mucin secretion induced by ATP'-a phenomenon simulating mucus overproduction from inflamed airway epithelial cells-by directly acting on airway mucin-secreting cells. Therefore, phellopterin should further be investigated for the possible use as mucoregulators in the treatment of inflammatory airway diseases.

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References

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