Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Viability of Bifidobacterial Strains against Acid, Bile Acid, and Oxygen Exposure

산, 담즙산, 산소 노출에 대한 비피도박테리아의 생존에 관한 연구

  • Published : 2006.12.31
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Abstract

Survival and stability of 19 bifidobacterial strains included 13 isolates from Korean infants against acid, bile acid and oxygen exposure were examined. Acid resistance of selected strains at pH values of 4.0, 3.0 and 2.0 was tested. Among the bifidobacterial strains tested, B. bifidum B3, B. longum D6, and B. adolescentis F1 exhibited higher viable cell counts exposed to acid whereas other strains had various results. The abilities of the strains to grow in the MRS broth containing 0.2% thioglycolic acid and 0.2% oxgall were tested and the tolerance of B. bifidum B3 and B. longum D6 to bile acid were higher than that of others. Even though in same species, the tolerance of tested strains to bile acid were variable. Stabilities of tested strains to oxygen exposure were variable and B. bifidum and B. longum strains showed relatively higher viable cell counts after 48 hours exposure to aerobic incubation. These results demonstrated that the survival and stability of bifidobacterial strains to acid, bile acid, and oxygen exposure were variable and strain-dependent. Due to their tolerant ability to environmental factors like acid, bile acid, and oxygen, B. bifidum B3 and B. longum D6 had good potential properties as probiotic cultures and may be useful for industrial application.

한국인 유아에서 분리한 13개 균주를 포함한 19개 bifidobacteria 균주를 대상으로 산, 담즙산 및 산소 노출시의 생균수의 변화를 측정하였다. 내산성은 pH 4.0, 3.0, 2.0에서 2시간까지 측정하였는데, 실험 균주마다 매우 상이한 결과를 나타내었으며, 실험 균주 중에서 B. bifidum B3, B. longum D6, B. adolescentis Fl이 우수한 생존력을 나타내었다. 실험 균주를 0.2% thioglycolic acid와 0.2% oxgall이 함유된 MRS 배지에서 생장을 측정한 결과, B. bififdum B3과 B. longum D6이 담즙산에 우수한 저항성을 나타냈으며, 균종간의 경향은 관찰되지 않았다. 호기 상태에서 48시간 노출시킨 후에 생균수의 변화를 측정한 결과, 실험 균주마다 상이한 결과를 나타내었으며 상대적으로 B. bifidum과 B. longum균주들의 생존력이 우수하였다. 산, 담즙, 산소 노출에 대한 생존력은 균종간의 경향을 나타내지 않고 균주마다 상이한 결과를 나타내었는데, 실험 균주 중에는 B. bifidum B3과 B. longum D6 균주가 모두 우수한 생존율을 나타내었다. 이들 균주는 현재 시판되는 상업용 종균과 비교해서도 동등한 성적을 나타내어 상업용 종균으로서의 사용 가능성을 시사하였다.

Keywords

References

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