Reproductive and Developmental Biology

  • 제30권4호
  • /
  • Pages.293-299
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  • 2006
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

Integration and Expression of Goat ${\beta}-Casein/hGH$ Hybrid Gene in a Transgenic Goat

  • Lee, Chul-Sang (Department of Biological Science, Kunsan National University) ;
  • Lee, Doo-Soo (Hanmi Research Center) ;
  • Fang, Nan-Zhu (Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Keon-Bong (Korea Research Institute of Bioscience and Biotechnology) ;
  • Shin, Sang-Tae (College of Veterinary Medicine, Chungnam National University) ;
  • Lee, Kyung-Kwang (Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2006.12.31
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초록

In order to generate transgenic goats expressing human growth hormone (hGH) in their mammary glands, goat ${\beta}-Casein/hGH$ hybrid gene was introduced into goat zygotes by pronuclear microinjection. DNA-injected embryos were transferred to the oviduct of recipients at 2-cell stage or to the uterus at morula/blastocyst stage after cultivation in glutathione-supplemented mSOF medium in vitro. Pregnancy and survival rate were not significantly different between 2-cell embryos and morula/blastocysts transferred to oviduct and uterus, respectively. One transgenic female goat was generated from 153 embryos survived from DNA injection. Southern blot analysis revealed that the transgenic goat harbored single-copy transgene with a partial deletion in its sequences. Despite of the partial sequence deletion, the transgene was successfully expressed hGH at the level of $72.1{\pm}15.1{\mu}g/ml$ in milk throughout lactation period, suggesting that the sequence deletion had occurred in non-essential part of the transgene for the transgene expression. Unfortunately, however, the transgene was not transmitted to her offspring during three successive breeding seasons. These results demonstrated that goat ${\beta}-casein/hGH$ gene was integrated into the transgenic goat genome in a mosaic fashion with a partial sequence deletion, which could result in a low level expression of hGH and a failure of transgene transmission.

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