Environmental Analysis Health and Toxicology

환경독성보건학회 (The Korean Society of Environmental Health and Toxicology)
권호 표지

비스페놀 에이와 에티닐에스트라디올에 노출된 키로노무스 4기 유충의 치사, 아치사 독성연구

Lethal and Sublethal Toxicities of Bisphenol A and Ethynyl Estradiol Exposure on the 4th Instar Larvae of Chironomus riparius (Diptera, Chironomidae)

  • 이세범 (서울시립대학교 환경공학부) ;
  • 최진희 (서울시립대학교 환경공학부)
  • Lee, Se-Bum (Faculty of Environmental Engineering, College of Urban Science, University of Seoul) ;
  • Choi, Jin-Hee (Faculty of Environmental Engineering, College of Urban Science, University of Seoul)
  • 발행 : 2006.12.31
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초록

본 연구에서는 내분비계장애물질로 잘 알려진 Bisphenol A와 Ethynyl estradiol(이하 BPA, EE)이 Chironomus riparius에 미치는 영향을 알아보고자 하였다. 수생생태계에서 Chironomus의 생태학적 중요성을 고려하여 연구한 반수치사농도(LC50),효소 활성, 성장/발달 등의 생화학적, 생리학적 독성에 대한 연구는 이들 물질에 대한 잠재적인 생태학적 중요성을 이해하는데 있어 유용한 정보를 제공할 수 있을 것이다. 연구 결과 BPA와 EE에 노출된 후 C. riparius게서 CAT와 GST활성이 증가하였고, EE에 고농도로 노출된 후 성충까지 충분히 성장하지 못했음을 확인하였다 이러한 결과로부터 C. riparius에서 BPA, EE에 대한 중요한 독성정보를 얻을 수 있었다. 그러므로 앞으로 BPA와 EE의 생화학적 효과와 C. riparitis의 개체수준 이상에서의 생리학적 실험을 통한 검증이 필요할 것이다.

키워드

참고문헌

  1. Bettinetti R, Cuccato D, Galassi S and Provini A. Toxicity of 4-nonylphenol in spiked sediment to three population of Chironomus riparius, Chemosphere 2002; 46(2): 201-207 https://doi.org/10.1016/S0045-6535(01)00114-X
  2. Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding, Anal Biochem 1976; 72: 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  3. Choi J, Roche H and Caquet T. Effects of physical (hypoxia, hyperoxia) and chemical (potassium dichromate, fenitrothion) stress on antioxidant enzyme activities in Chironomus riparius Mg. (Diptera, Chironomidae) larvae: potential biomarkers, Environ Toxicol Chem 2000; 19(2): 495-500 https://doi.org/10.1897/1551-5028(2000)019<0495:EOPHHA>2.3.CO;2
  4. Choi J, Caquet T and Roche H. Multilevel effects of sublethal fenitrothion exposure in Chironomus riparius Mg. (Diptera, Chironomidae) larvae, Environ Toxicol Chem 2002; 21(12): 2725-2730 https://doi.org/10.1897/1551-5028(2002)021<2725:MEOSFE>2.0.CO;2
  5. Colborn T, Myers JP and Dummanoski D. Our Stolen Future, Little Brown and Co, Boston. 1996
  6. Crane M, Sildanchandra W, Kheir R and Callaghan A. Relationship between biomarker activity and developmental endpoints in Chironomus riparius Meigen exposed to an organophosphate insecticide, Ecotoxicol Environ Saf 2002; 53(3): 361-369 https://doi.org/10.1016/S0147-6513(02)00038-6
  7. Day K and Scott I. Use of Acetylcholinesterase activity to detect sublethal toxicity in stream invertebrates exposed to low concentrations of organophosphorus pesticides, Aquat Toxicol 1990; 18(2): 101-113 https://doi.org/10.1016/0166-445X(90)90021-G
  8. Day KE, Kirby R and Reynoldson TB. Sexual dimorphism in Chironomus riparius (Meigen): impact on interpretation of growth in whole-sediment toxicity tests, Environ Toxicol Chem 1994; 13: 35-39 https://doi.org/10.1897/1552-8618(1994)13[35:SDICRM]2.0.CO;2
  9. Detra R and Collins W. The relationship of parathion concentration, exposure time, cholinesterase inhibition and symptoms of toxicity in midge larvae (Chironomidae: Diptera), Environ Toxicol Chem 1991; 10: 1089-1095 https://doi.org/10.1897/1552-8618(1991)10[1089:TROPCE]2.0.CO;2
  10. Ellman GL, Courtney KD, Andres V Jr and Feather-stone RM. A new and rapid colorimetric determination of acetylcholinesterase activity, Biochem Pharmacol 1961; 7: 88-95 https://doi.org/10.1016/0006-2952(61)90145-9
  11. George P. Intermediate Compound formation with peroxidase and strong oxidizing agents, J Biol Chem 1953; 201(1): 413-426
  12. Habig WH, Pabst MJ and Jakoby WB. Glutathione Stransferases. The first enzymatic step in mercapturic acides formation, J Biol Chem 1974; 249(22): 7130-7139
  13. Ibrahim H, Kheir R, Helmi S, Lewis J and Crane M. The effects of organophosphorus, carbamate, pyrethroid and organochlorine pesticides and a heavy metal on survival and cholinesterase activity of Chironomus riparius (Meigen), Bull Environ Contam Toxicol 1998; 60(3): 448-455 https://doi.org/10.1007/s001289900646
  14. Ingersoll C and Nelson MK. Testing sediment toxicity with Hyalella azteca (amphipod) and Chironomus riparius (Diptera). In: Landis W, Van der Schalie W (Eds.), Aquatic Toxicology and Risk Assessment. American Society for Testing and Materials, Philadelphia, 1990; 13: 93-109
  15. Kahl MD, Makynen EA, Kosian PA and Ankley GT. Toxicity of 4-nonylphenol in a life-cycle test with the midge Chironomus tentans, Ecotoxicol Environ Saf 1997; 38 (2): 155-160 https://doi.org/10.1006/eesa.1997.1572
  16. Karnak RE and Collins WJ. The susceptibility to selected insecticides and acetylcholinesterase activity in a laboratory colony of midge larvae, Chironomus tentans (Diptera: Chironomidae), Bull Environ Contam Toxicol 1974; 12(1): 62-69 https://doi.org/10.1007/BF01713027
  17. Kosalwat P and Knight AW. Chronic toxicity of copper to a partial life cycle of the midge, Chironomus decorus, Arch Environ Contam Toxicol 1987; 16: 283-290 https://doi.org/10.1007/BF01054945
  18. Lasson DGJ, Adofsson-Erici M, Parkkonen J, Petterson M, Berg AH, Olsson PE and Forlin L. Ethinylestradiol-an undesired fish contraceptive, Aquat Toxicol 1999; 45(2-3): 91-97 https://doi.org/10.1016/S0166-445X(98)00112-X
  19. Matthew MW and David P. Selection of an appropriate life cycle stage of Chironomus riparius meigen for use in chronic sediment toxicity testing, Chemosphere 1998; 36(6): 1405-1413 https://doi.org/10.1016/S0045-6535(97)10041-8
  20. Matthew MW, David P and Kathleen C. Chronic exposure to $17{\alpha}-thinylestradiol$ and bisphenol A-effects on development and reproduction in the freshwater invertebrate Chironomus riparius (Diphtera: Chironomidae), Aquat Toxicol 2001; 55(1-2): 113-124 https://doi.org/10.1016/S0166-445X(01)00214-4
  21. Paglia DE and Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathion peroxidase, J Lab Clin Med 1967; 70(1): 158-169
  22. Pascoe D, William KA and Green DWJ. Chronic toxicity of cadmium to Chironomus riparius Meigen-effects upon larval development and adult emergence, Hydrobiologia 1989; 175(2): 109-115 https://doi.org/10.1007/BF00765121
  23. Purdom CE, Hardiman PA, Bye VJ, Eno NC, Tyler CR and Sumpter J. Estrogenic effects of effluents from sewage treatment works, Chem Ecol 1994; 8: 275-285 https://doi.org/10.1080/02757549408038554
  24. Staples CA, Dorn PB, Klecka GM, O'Block ST and Haris LR. A review of the environmental fate, effects and exposures of bisphenol A, Chemosphere 1998; 36(10): 2149-2173 https://doi.org/10.1016/S0045-6535(97)10133-3
  25. Taylor EJ, Blockwell SJ, Maund SJ and Pascoe D. Effects of lindane on the life-cycle of a freshwater macroinvertebrate Chironomus riparius Meigen (Insecta: Diptera), Arch Environ Contam Toxicol 1993; 24: 145-150 https://doi.org/10.1007/BF01141340