Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibition of Calcium Transport by $(1R,9S)-\beta-Hydrastine$ Hydrochloride in PC12 Cells

  • Yin, Shou-Yu (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University) ;
  • Lee, Myung-Koo (College of Pharmacy, and Research Center for Bioresource and Health, Chungbuk National University)
  • Published : 2006.12.30
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Abstract

The effects of $(1R,9S)-\beta-hydrastine$ hydrochloride (BHSH) on $Ca^{2+}$ transport in rat pheochromocytoma PC12 cells were investigated. In the presence of external $Ca^{2+}$, BHSH at $100{\mu}M$ inhibited $K^+$ (56mM)-induced dopamine release, and $K^+-induced$ $Ca^{2+}$ influx and a sustained rise of $[Ca^{2+}]_i$. In addition, BHSH at 100 f.!M reduced the sustained rise of $[Ca^{2+}]_i$ elicited by 20 mM caffeine, but not by $1{\mu}M$ thapsigargin, in presence of external $Ca^{2+}$. These results suggest that BHSH inhibited $K^+-induced$ dopamine release and $[Ca^{2+}]_i$ influx, and store-operated $Ca^{2+}$ channels activated by caffeine, but not by thapsigargin, in PC12 cells.

Keywords

References

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