Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effects of Araliaceae Water Extracts on Blood Glucose Level and Biochemical Parameters in Diabetic Rats

두릅나무과 열수추출물이 당뇨흰쥐의 혈당과 혈액 성분에 미치는 영향

  • Published : 2006.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the effects of Aralia elata, Acanthopanacis cortex and Ulmus davidiana water extracts on blood hemoglobin, $HbA_{1c}$, levels and biomarkers in the streptozotocin (STZ)-induced diabetic rats. Male Wistar rats divided into normal and diabetic groups. The diabetic groups subdivided into the control group (DM) and Araliaceae water extracts supplemented groups: Aralia elata (AE), Acanthopanacis cortex (AC) and Ulmus davidiana (UD). The extracts were supplemented in diet base on 11.42 g of raw Araliaceae/kg diet for 7 weeks. The diabetes was induced by injecting 572 (55 mg/kg B.W., i.p.) once 2 weeks before sacrifying. Relative weights of liver were significantly lowered in the DM group compared to the normal group, whereas those of kidney and heart were significantly increased in the DM group. Supplementation of the Araliaceae water extracts improved reduced liver weights in STZ-induced diabetic rats. Blood glucose level was significantly higher in the DM group than in the normal group, whereas insulin contents were significantly lowered in the DM groups. However, these parameters were normalized in the An, AC and UD supplemented groups, respectively. Blood hemoglobin and $HbA_{1c}$ levels were significantly higher in the DM group than in the normal group. When all of Araliaceae water extracts were supplemented to the diabetic rats lowered hemoglobin and $HbA_{1c}$ levels. Red blood cell, white blood cell and Lymphocyte were significantly higher in the DM group than in the normal group. The supplementation of Araliaceae family water extracts significantly lowered these parameters compared to the DM group. MCV, MCH contents were declined in the DM group, while the supplementation of Aralia elata, Acanthopanacis cortex and Ulmus davidiana water extracts elevated of these contents in STZ-induced diabetic rats. Accordingly, these results indicate that Aralia elata, Aeanthopanacis corex and Ulmus davidiana water extracts would seem to improve the blood biomarkers in STZ-induced diabetic rats.

Keywords

References

  1. Sakurai T, Tsuchiya S. Superoxide production from nonenzymatically glycated protein. FEBS Lett 236: 406-410, 1988 https://doi.org/10.1016/0014-5793(88)80066-8
  2. Mullakey CJ, Edelstein D, Brownless M. Free radical generation by early glycation products: a mechanism for accelerated atherogenesis in diabetes. Biochem Biophys Res Commun 173: 932- 939, 1990 https://doi.org/10.1016/S0006-291X(05)80875-7
  3. 김경래. 당뇨병 민간요법의 실태. 당뇨병 18(부록 1): 61- 64, 1994
  4. 김윤숙, 전진호, 박정현, 강창일. 당뇨병 환자들의 대체의학 경험실태와 관련요인. 당뇨병 2(4): 78-89, 2000
  5. Pack IC, Young HS, Choi JS. Constituents of Cudrania tricuspidata in Korea. Yakhak Hoeji 36: 40-45, 1992
  6. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. Polyphenolic compound content and antioxidant activities by extracts from seed, sprout and flower of saffower (Carthamus tinctorius L). J Korean Soc Food Nut 27: 1217-1222, 1998
  7. Cha JY, Cho YS. Effect of potato polyphenolics on lipid peroxidation in rats. J Korean Soc Food Nutr 28: 1231-1136, 1999
  8. Park JC, Choi JS, Cho JW. Effects of the fraction from the leaves, fruits, stems and roots of Cudrania tricuspidata and flavonoids on lipid peroxidation. Kor J Pharmacogn 26: 377-384, 1995
  9. 許 逡. 동의보감 (東醫寶鑑). 南山堂. 서울: 446-506, 1989
  10. 지형준, 김현수. 생약의 Saponin 성분에 관한 연구 (제2보). 해동피의 Saponin 성분. 서울대 생약연구소 업적집 22: 121- 128, 1983
  11. Han BH, Park MH. Studies on the antiinflammatory activity of Aralia continentalis. Arch Pharm Res 6(1): 75-77, 1983 https://doi.org/10.1007/BF02855695
  12. Bohn B, Nebee CT, Birr C. Arzneim Forch/Drug Res 27: 1193- 1200, 1987
  13. Ovodov YS, Frolova GM. Triterpenoid glycosides of Eleuterococcus senticosus leaves. II. Khim Prirodnih Soedin 1: 618-622, 1971
  14. Hikino H, Takahashi M, Otaka K, Konnon C. Isolation and hyperglycemia activity of eleutherans A, B, C, D, E, F and G: Glycans of eleucoccus sentico roots. J of Natural Products 49: 293-297, 1986 https://doi.org/10.1021/np50044a015
  15. 홍사악, 박찬웅, 신금봉. 오가피성분이 흰쥐조건 희피반응에 미치는 영향. 서울의대잡지 13: 41-44, 1972
  16. Kim CH, Han DY. The biological acitivity of a new glycoside, chisanoside from Acanthopanax chiisanesis Nakai Leaves. Arch Pharmacal Res 24(2): 123-134, 1980
  17. Park JS, Shim CJ, Jung JH, Lee GH, Sung CK, Oh MJ Antimicrobial activity of Ulmi cortex extracts. J Korean Soc Food Sci Nutr 28: 1022-1028, 1999
  18. Lee YJ, Han JP. Antioxidative activities and nitrite scavenging ability of extracts from Ulmus devidiana. J Kor Soc Nutr 29 (5) : 893-899, 2000
  19. 김경래. 당뇨병 민간요법의 실태. 당뇨병 18 (부록 1): 61-64, 1994
  20. Rakieten N, Gordon BS, Cooney DA, Davis RD, Schein PS. Renal tumorgenic action of streptozotocin (NSC-85998) in rats. Cancer Chemother Rep 52: 563-567, 1968
  21. American Institute of nutrition. Report of the American Institute of nutrition Ad Hoc committee on standards for nutritional studies. J Nutr 107: 1340-1348, 1977 https://doi.org/10.1093/jn/107.7.1340
  22. Koch CD. Determination of glucose with glucose oxidase-UV, using hexokinase as the reference method. J Clin Chem Clin Biochem 14: 373-374, 1976
  23. Desbuquois B, Aurbach GB. Use of polythylene glycol to separate free and antibody bound peptide hormones in radioimmunoassays. J Clin Endocrinol Metab 33: 732-738, 1971 https://doi.org/10.1210/jcem-33-5-732
  24. AOAC. Official methods of analysis. 15th ed. Association of analytical chemists, Washington DC, 1990
  25. Price ML, Butler LG. Rapid visual examination and spectrophotometric determination of tannin content of sorghum grain. J Agri Food Chem 25: 1268-1275, 1977 https://doi.org/10.1021/jf60214a034
  26. Goldstein DE, Little RR, Wiedmeyer HM, England JD, McKenzie EM. Glycated hemoglobin: methodologies and clinical applications. Clin Chem 32: B64-B70, 1986
  27. Power FB, Browing H. The constituents of Taraxacum root. J Chem Soc Transactions 101: 2411-2429, 1912 https://doi.org/10.1039/ct9120102411
  28. Noguchi N, Niki E. Phenolic antioxidant:a rational for design and evaluation of novel antioxidant drug for atherosclerosis. Free Radic Bio Med 28(10) : 1538-1546, 2000 https://doi.org/10.1016/S0891-5849(00)00256-2
  29. Yoshikawa M, Murakami T, Harada E, Murakami N, Yamikara J, Matsuda H. Bioactivity saponins and glycosides. VII. On the hypoglycemic principles from the root cortex of Aralia elata Seem: structure related hypoglycemic activity of oleanolic acid oligoglycoside. Chem Pharm Bull(Tokyo) 44: 1923, 1996
  30. Todorov IN, Sizova ST, Kosagonova NY, Mitrokhin IN, German AV, Mitrofanova MA. Pharmacokinetics and mechanism of action of glycosides of eleutherococci. Effects of extract on the metabolism and biosynthesis of protein in several organs and tissues of rats. Khim Farm Zh 18: 529-533, 1984
  31. Socher M, Kunjara S, Baquer NZ, Mclean P. Regulation of glucose metabolism in livers and kidneys of NOD mice. Diabetes 40: 1467-1471, 1991 https://doi.org/10.2337/diabetes.40.11.1467
  32. Han HK, Lim SJ. Effects of fractions from methanol extract of Commelina communis on blood glucose levels energy metabolism in streptozotocin-induced diabetic rats. Korean J Soc Food SCI 14(5): 577-583, 1998
  33. Day C. The allium aliiance. Nutr Food Sci 90: 20-21, 1984
  34. Kamel MS, Ohtant K, Kurokawa T, Assaf M, El-Shanawany MA, Ali AA, Kassi R, Ishibashi SS, Tanaka O. Studies on Balanites aegyptiaca fruits an antidiabetic Egyptian folk medicine. Chem Pharm Biophys 32: 106-108, 1995
  35. Kimura Y, Okuda H, Arichi S. Effects of extracts of Ganoderma lucidum on blood glucose level in rats. Planta Medica 54: 290- 294, 1988 https://doi.org/10.1055/s-2006-962435
  36. 박성우. 인슐린 비의존형 당뇨병 환자에서 인슐린 분비능에 대한 공복 시 혈당의 영향. 당뇨 병 16(4). 한림대학교 의과대학. 서울, pp.113-121, 1992
  37. Berlin N, Berk P. The biological life of the red cell. In: Surgenor DM ed. The red blood cell II. Academic Press New York, pp.991-1005, 1975
  38. Eser YS, Bulent SY, Dlene, Taner O. Catalase/Superoxide Dismutase (SOD) and catalase/paraoxonase (PON) ratio May implicate Glycemic Control. Archives of Medical Research 32: 283- 287, 2004 https://doi.org/10.1016/S0188-4409(01)00285-5
  39. 이영미. 두릅나무와 황백 혼합추출물이 streptozotocin으로 당뇨를 유도한 백서의 신장과 수정체에서 항산화 효소계와 폴리올 대사에 미치는 영향. 서울대학교 대학원 가정학석사학위 논문, 1998
  40. Rahbar S. An abnormal hemoglobin in red cells of diabetics. Clin Chem Acta 22: 296-298, 1968 https://doi.org/10.1016/0009-8981(68)90372-0
  41. Iberg N, Fluckiger R. Nonenzymatic glycation of albumin in vivo (identification of multiple glycosylated site). J Biol Chem 261: 13542-13545, 1986
  42. Khim. Eleutherociccus glycosides on inclusion of labelled phosphorus in m-RNA. Veprosy Med 17: 267, 1972
  43. Winter CA, Risley EA, Nuss GW. Carrageenan-induced edema in hide paw of the rat as an assay for antiinflammatory drugs. Proc Soc Exp Biol Med 111: 544, 1962
  44. Symeonidis A, Athanassiou G, Psiroyannis A, Kyriazopoulou V, Kapatais K, Zoumbos M, Zoumbosm N. Impairment of erythrocyte viscoelasticity is correlated with levels of glycosylated hemoglobin in diabetic patients. Clin Lab Haem 23: 103-109, 2001 https://doi.org/10.1046/j.1365-2257.2001.00366.x
  45. Venerando B, Fiorilli A, Croci G, Tringali C, Goi G, Mazzanti L, Curatola G, Segalini G, Massaccesi L, Lombardo A, Tettamati G. Acidic and neutral sialidase in the erythrocyte membrane of type 2 diabetic patients. Blood 99: 1064-1070, 2002 https://doi.org/10.1182/blood.V99.3.1064
  46. Bessman JD, Gilmer PR, Gardner FH. Improved classification of anemias by MCV and RDW. Am J Clin Path 80: 322-326, 1983 https://doi.org/10.1093/ajcp/80.3.322
  47. Yi KN, Lee CS. Clinical pathology file. Euihak Munwhasa Co. Second Edition. Seoul, Korea, 1993