Eco-frendly Control of Culex pipiens (mosquito) Larvae by Acorus calamus (sweet flag) and Acorus gramineus (Grassy-leaved sweet flag) Extracts

  • Choi, Jeong-Keun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Lee, Ji-Yeon (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Lee, Ja-Hyun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Lee, Seong-Gene (Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Han, Yeon-Soo (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Han, Tae-Ho (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University)
  • 발행 : 2006.12.30

초록

Mosquitoes are carriers of malaria and encephalitis. This study performed for eco-friendly control of mosquitos by using genus Acorus. Several solvents were used for the extraction of genus Acorus; water, ethanol, and methanol. Grinded leaves and roots were also included. Acorus extracts killed mosquito larvae and the ethanol extract showed the best result. Autoclaved Acorus water needed long time to kill mosquito larvae. $LT_{50}$ of 1 % Acorus calamus decoction was 13.6 hrs and 1 % autoclaved Acorus water was 53.6 hrs. $LT_{50}$ of 0.05% Acorus calamus rhizome powder was 28.5 hrs. $LT_{50}$ of 0.5% Acorus calamus leaf powder was 10.8 hrs. $LT_{50}$ of 0.1 % Acorus calamus decoction was 63.4 hrs and 0.1 % Acorus calamus ethanol extracts was 48.6 hrs and 0.1% Acorus calamus methanol extracts was 53.9 hrs. $LT_{50}$ of 0.4% Acorus gramineus decoction was 45.5 hrs, 0.4% ethanol extracts was 10.9 hrs, 0.4% methanol extracts was 10.2 hrs. $LT_{50}$ of ethanol extracts was shorter than other extracts. Acorus calamus rhizome powder could be used for the eco-friendly control of the mosquito larvae.

키워드

참고문헌

  1. Burfield, T. and S.L. Reekie. 2005.Mosquitoes,malaria and essential oils. The International J. Aromatherapy 15: 30-41 https://doi.org/10.1016/j.ijat.2004.09.013
  2. Cetin,H., F. Erler andA.Yanikoglu. 2004. Larvicidal activity of a botanical natural product, AkseBio2, against Culex pipiens. Fitoterapia 75: 724-728 https://doi.org/10.1016/j.fitote.2004.09.003
  3. Cheng, S.S.,H.T. Chang, S.T. Chang,K.H. Tsai andW.J. Chen. 2003. Bioactivity of selected plant essential oils against the yellowfever mosquito Aedes aegypti larvae. Bioresource Technol. 89: 99-102 https://doi.org/10.1016/S0960-8524(03)00008-7
  4. Dharmagadda,V.S.S., S.N.Naik, P.K.Mittal and P.Vasudevan. 2005. Larvicidal activity of Tagets patula oil against three mosquito species. Bioresource Technol. 96: 1235-1240 https://doi.org/10.1016/j.biortech.2004.10.020
  5. Guillet, G., B.J.R. Philogene, J. O'Meara, T. Durst and J.T. Arnason. 1997.Multiplemodes of insecticidal action of three classes of polyacetylene derivatives from Rudbeckia hirta. Phytochemistry 46; 495-498 https://doi.org/10.1016/S0031-9422(97)00320-8
  6. Hwang, M.S. 2004. Hair protective effect of water extracts from Acorus calamus leaf. Department of health sciences. The graduates school of health sciences. Catholic Univ. of Daegu. Thesis
  7. Jeon, J.S.,M.J. Kwon,S.H.O., H.J. Nam, H.Y. Kim, J.M. GoandY.H. Kim. 2006.Asurvey on the pesticide residues on agricultural products on the markets in Incheon area from 2003 to 2006. Kor. J.Environ. Agri. 25: 180-189 (in Korean) https://doi.org/10.5338/KJEA.2006.25.2.180
  8. Kang, S.H.,M.K.Kim,D.K. Seo and G.H.Kim. 2006. Insecticidal activity of essential oils against Larvae of Culex pipiens pallens.Kor. J. Pesticide Sci. 10: 43-49
  9. Kim S.I., OK. Shin, C. Song, K.Y. Cho and Y.J. Ahn. 2001. Insecticidal activities of aromatic plant extracts against four agricultural insects. Agric. Chem. Biotechnol. 44(1): 23-26
  10. Kim, H.J., S.W. Kimand C.S. Shin. 2000. Analysis of chemical composition in leaf and root of Acorus calamus L. Kor. J. Food Sci.Technol. 32: 37-41 (in Korean)
  11. Lee, D.K. 2006. Occurrence of Culex pipiens (Diptera, Culicidae) and effect of vent net sets for mosquito control at septic tanks in south-eastern area of theKorean peninsula.Kor. J. Appl. Entomol. 45: 51-57 (in Korean)
  12. Lee, H.D., O.J. You, Y.B. Ihm, H.Y. Kwon, Y.D. Jin, J.B. Kim, Y.H. Kim, S.S. Park, K.S. Oh, S.L.Ko, T.H. Kim, J.G.Noh, K.Y. Chung and K.S. Kyung. 2006. Residual characteristics of some pesticides in/on pepper fruits and leaves by different types, growing and processing conditions. J. Pesticide Sci. (10): 99-106
  13. Lee.G.D., S.A. Lee,K.J. Son,Y.S. Lee, J.G. Jeon and K.W. Chan. The antibacterial effect of Acorus calamus var. angustatusBesser on the mutans streptococci. J. Kor. Acad Dent. Health 28: 153-160
  14. Luna, J. de S., A.F. dos Santos, M.R.F. de Lima, M.C. de Omena, F.A.C. de Mendone, L.W. Bieber and A.E.G. Sant'Ana. 2005. A study of the larvicidal andmolluscicidal activities of somemedicinal plants from northeast Brazil. J. Ethnopharmacology 97: 199-206 https://doi.org/10.1016/j.jep.2004.10.004
  15. Markouk, M., K. Bekkouche, M. Larhsini, M. Bousaid, H.B. Lazrek andM. Jana. 2000. Evaluation of someMoroccan medicinal plant extracts for larvicidal activity. J. Ethnopharmacology 73: 293-297 https://doi.org/10.1016/S0378-8741(00)00257-9
  16. Medonca, F.A.C., K.F.S. de Silva, K.K. dos Santos, K.A.L. Ribeiro Junior and A.E.G. Sant'Ana. 2005. Activities of some Brazilian plants against larvae of themosquito Aedes aegypti. Fitoterapia 76: 629-636 https://doi.org/10.1016/j.fitote.2005.06.013
  17. Nathan, S.S.,K.Kalaivani andK.Murugan. 2005. Effects of neemlimonoids on the malaria vector Anopheles stephensi Liston (Diptera: Culicidae). Acta Tropica 96: 47-55 https://doi.org/10.1016/j.actatropica.2005.07.002
  18. Ndung'u, M., A. Hassanali, A.M. Hooper, S. Chhabra, T.A. Miller, R.L. Paul and B. Torto. 2003. Ring A-seco mosquito larvicidal limonoids from Turraea wakefieldii. Phytochem. 64: 817-823 https://doi.org/10.1016/j.phytochem.2003.08.020
  19. Oh, S.Y., S.T.Choi, J.G. KimandC.I. Lim. 2005.Removal effects of washing treatments on pesticide residues and microorganisms in leafy vegetables. Kor. J. Hort. Sci. Technol. 23: 250-255 (in Korean)
  20. Phongpaichit, S., N. Pujenjob, V. Rukachaisirikul andM. Ongsakul . 2005. Antimicrobial activities of the crude methanol extract of Acorus calamus Linn. Songklanakarin J. Sci. Technol. 27(2): 517-523
  21. Prakash, A. and J. Rao 1997. Botanical pesticides in agriculture. Lewis Publishers, CRC Press, Inc
  22. Rahuman. A.A., G. Gopalakrishnan, B.S. Ghouse, S. Arumugamand B.Himalayan. 2000. Effect of Feronia limonia onmosquito larvae. Fitoterapia 71: 553-555 https://doi.org/10.1016/S0367-326X(00)00164-7
  23. Rajkumar, S. andA. Jebanesan. 2004.Mosquitocidal activities of octacosane from Moschosma polystachyum Linn. (lamiaceae) J.Ethno-Pharm. 90: 87-89 https://doi.org/10.1016/j.jep.2003.09.030
  24. Shaalan, E.A.S., D. Canyon, M.W.F. Younes, H. Abdel-Wahab and A.H. Monsour. 2005. Areview of botanical phytochemicals woth mosquitocidal potential. Environ. International 31: 1149-1166 https://doi.org/10.1016/j.envint.2005.03.003
  25. Thomas, C.J. and A. Callaghan. 1999. The use of garlic (Allium sativa) and lemon peel (Citrus limon) extracts as Culex pipiens larvacides; persistence and interaction with an organophosphate resistance mechanism. Chemosphere 39: 2489-2496 https://doi.org/10.1016/S0045-6535(99)00161-7
  26. Vanderherchen, M.B., M. Isherwood, D.M. Thompson, R.J. Linderman and R.M. Roe. 2005. Toxicity of novel aromatic and aliphatic organic acid and ester analogs of trypsin modulating oostatic factor to larva of the northern housemosquito, Culex pipiens complex and the tobacco hornworm, Manduca sexta. Pesticide Biochem. Physiol. 81: 71-84 https://doi.org/10.1016/j.pestbp.2004.09.006
  27. Wongtong S. and S.Nawanich. 2001. Some insecticidal plant extracts for controlling maize weevil, Sitophilus zeamais Motschulsky (Coleoptera : Curculionidae). Kasetsart J. (Nat. Sci.) 35: 259-270