Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Construction of an Escherichia-Pseudomonas Shuttle Vector Containing an Aminoglycoside Phosphotransferase Gene and a lacZ' Gene for $\alpha$-Complementation

  • Lee, Bheong-Uk (Division of Biological Sciences, Kosin University) ;
  • Hong, Ja-Heon (Division of Biological Sciences, Kosin University) ;
  • Kahng, Hyung-Yeel (Department of Environmental Education, Sunchon National University) ;
  • Oh, Kye-Heon (Department of Genetic Engineering, Soonchunhyang University)
  • Published : 2006.12.31
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Abstract

A new 4.87 kb Escherichia-Pseudomonas shuttle vector has been constructed by inserting a 1.27 kb DNA fragment with a replication origin of a Pseudomonas plasmid pRO1614 into the 3.6 kb E. coli plasmid pBGS18. This vector, designated pJH1, contains an aminogly-coside phosphotransferase gene (aph) from Tn903, a lacZ' gene for $\alpha$-complementation and a versatile multiple cloning site possessing unique restriction sites for EcoRI, SacI, KpnI, SmaI, BamHI, XbaI, SalI, BspMI, PstI, SphI, and HindIII. When pJH1 was transformed into E. coli DHS${\alpha}$ and into P. putida HK-6, it was episomally and stably maintained in both strains. In addition, the enhanced green fluorescent protein (EGFP) gene which was transcriptionally cloned into pJH1 rendered E. coli cells fluorescence when its transformants were illuminated at 488 nm.

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References

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