Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Tumor Necrosis factor-α Promotes Osteogenesis of Human Bone Marrow-derived Mesenchymal Stem Cells through JNK-dependent Pathway

Tumor necrosis factor-α에 의한 골수 유래 중간엽 줄기세포의 골세포로의 분화 촉진에서 JNK의 역할

  • Kim, Mi-Ra (Medical Research Center for Ischemic Tissue Regeneration & Medical Research Institute) ;
  • Song, Hae-Young (Medical Research Center for Ischemic Tissue Regeneration & Medical Research Institute) ;
  • Kim, Jae-Ho (Medical Research Center for Ischemic Tissue Regeneration & Medical Research Institute)
  • 김미라 (부산대학교 의과대학 생리학교실) ;
  • 송해영 (부산대학교 의과대학 생리학교실) ;
  • 김재호 (부산대학교 의과대학 생리학교실)
  • Published : 2006.12.01
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Abstract

Tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ has been implicated in skeletal diseases by promoting bone loss in inflammatory bone diseases. In the present study, we examined the effects of $TNF-{\alpha}$ on osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). $TNF-{\alpha}$ dose-dependently promoted matrix mineralization of hBMSCs with a maximal stimulation at 2ng/ml. $TNF-{\alpha}$ increased expression of alkaline phosphatase, which plays a crucial role for the matrix deposition. The $TNF-{\alpha}-stimulated$ osteoblastic differentiation was not affected by $NF_kB$ inhibitors, BAY and SN50. However, a JNK-specific inhibitor, SP600125 completely abolished the $TNF-{\alpha}-stimulated$ matrix mineralization and expression of alkaline phosphatase. These results suggest that $TNF-{\alpha}$ enhances osteoblastic differentiation of hBMSCs through JNK-dependent pathway.

Tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$는 염증성 골질환에서의 골조직의 손실과 밀접한 관련이 있다. 본 연구에서는 인체 골수 유래 중간엽 줄기세포의 골세포로의 분화과정에 대한 $TNF-{\alpha}$의 영향을 조사하였다. $TNF-{\alpha}$는 골수 유래 중간엽 줄기세포의 골세포로의 분화를 나타내는 표시인 세포외 무기질 축적과 alkaline phosphatase의 발현의 증가를 일으켰으며 2ng/ml의 농도에서 최대의 증가를 나타내었다. $TNF-{\alpha}$에 의한 골세포로의 분화는 $NF_kB$의 저해제에 의해서는 영향받지 않았으나 JNK 특이 저해제인 SP600125에 의해 완벽하게 억제되었다. 이는 $TNF-{\alpha}$에 의한 골수 유래 중간엽 줄기세포의 골세포로의 분화과정에 JNK가 중요한 역할을 한다는 것을 제시한다.

Keywords

References

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