Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Cytohistological Study of Development of Callus and Adventitious Shoots from Cultured Stem of Vigna radiata

녹두 줄기 조직배양에서 캘러스와 부정아 형성에 관한 세포조직학적 연구

  • Published : 2006.12.01
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Abstract

This study was carried out to establish a reproducible culture system for callus formation and adventitious shoot development from young stem segments of Vigna radinta, and histological work for orgin of callus tissue and adventitious shoot. Induction of callus from young stem explants of Vigna radiata was very effective on MS inorganic salts supplemented with 0.5 mg/L 2,4-D and 1.0 mg/L kinetin. For the adventitious shoot regeneration from the callus tissues, the hormone combination of 0.75 mg/L NAA, 1.5 mg/L kinetin and MS salts resulted in about 21% efficiency. Histological examination showed that callus tissues originated from out-growths by callus cambium rings with do novo meristematic activities, which were localized at the outside of the vascular cambium. Adventitious shoots were developed from shoot apical meristem originated from the surface of callus masses. The shoot apical meristem produced leaf primordium, which then became leaf.

녹두 유식물체 줄기를 조직배양하여 캘러스조직을 형성하였고 이로부터 부정아 형성을 유도한 다음, 캘러스 조직과 부정아 분화에 대한 기원을 조직해부학적으로 연구하였다. 녹두 줄기절편으로부터 캘러스조직의 유도는 0.5 mg/L 2,4-D와 1.0 mg/L kinetin이 첨가된 MS배지가 효과적이었고, 캘러스로부터의 부정아 분화에는 0.75 mg/L NAA와 1.5 mg/L kinetin이 첨가된 MS배지가 매우 효과적이어서 약 21%의 기관형성율을 나타내었다. 캘러스 조직을 조직 학적으로 관찰한 결과, 캘러스조직은 유관속 형성층 외측에 새롭게 형성된 분열능력이 있는 캘러스 형성층환인_바깥쪽으로 생장을 함으로써 유도되었다. 부정아는 캘러스 조직의 외부 표면부위에서 기원된 부정아 정단분열조직으로부터 발생되었다. 부정아 정단분열조직은 엽원기를 형성하여 나중에 잎이 발생되었다.

Keywords

References

  1. Amutha, S., A. Ganapathi and M. Muruganantham. 2003. In vitro organogene-sis and plant formation in Vigna radiata (L.) Wilczek. Plant Cell, Tiss. Org Cult. 71, 203-207
  2. Amutha, S., M. Muruganantham and A. Ganapathi. 2006. Thidiazuron-induced high-frequency axillary and adventitious shoot regeneration in Vigna radiata (L.) Wilczek. In Vitro Cell. Dev. Biol. Plant 42, 26-30 https://doi.org/10.1079/IVP2005721
  3. Attfield, E. M. and P. K. Evans. 1991. Stages in the initiation of root and shoot organogenesis in the cultured leaf explants of Nicotiana tabacum cv. Xanthi nc. J. of Experimental Bot. 42, 59-63 https://doi.org/10.1093/jxb/42.1.59
  4. Bajaj, Y. P. S. and H. Singh. 1980. In vitro induction of androgenesis in mung bean (Phaseolus aureus). Indian J. Exp. Biol. 18, 1316-1318
  5. Baraldi, R, F. Rossi and B. Lercari. 1988. In vitro shoot development of Prunus GF 655-2: interaction between light and benzyladenine. Physiol. Plant. 74, 440-443 https://doi.org/10.1111/j.1399-3054.1988.tb02000.x
  6. Boulter, D. and O. J. Crocomo, 1979. Plant cell culture implications:Legumes, pp. 614-631, In Sharp, W. R., P. O. Larsen, E. F. Paddock, V. Raghavan, (eds.), Plant Cell and Tissue Culture, Ohio State University Press, Columbus
  7. Cheng, T. Y. 1975. Adventitious bud formation in culture of Douglas fir (Pseudotsuga menziensii Mirb. Franco). Plant Sci. Lett. 5, 97-102 https://doi.org/10.1016/0304-4211(75)90049-8
  8. Crocomo, O. J., J. E. Peters and W. R. Sharp. 1976a. Plantlet morphogenesis and the control extract. Z. Pflanzenphysiol. 78S, 456-460
  9. Crocomo, O. J., J. E. Peters and W. R. Sharp. 1976b. Interactions of phytohormones on the control of growth and root morphogenesis in cultured Phaseolus vulgaris leaf explants. Turrialba 26, 232-236
  10. Echenique, V., P. Polci and L. Mroginski. 1996. Plant regeneration in weeping lovegrass, (Eragrostis curvula) through inflorescence culture. Plant Cell, Tiss. Org Cult. 46, 123-130 https://doi.org/10.1007/BF00034845
  11. Evans, D. A., W. R. Sharp and E. F. Paddock. 1976. Variations in callus proliferation in leaf tissue cultures of Glycine max strain T219. Phytomorphology 26, 379-381
  12. Flick, C. E., D. A. Evans and W. R. Sharp. 1983. Organogenesis, pp 13-81 In Evans, D. A. W. R. Sharp, P. V. Arumirato and Y. Yamada (eds.), Handbook of Plant Cell Culture. Vol. 1, Macmillan Publishing Co., New York
  13. Geneve, R. L. 1991. Patterns of adventitious root formation in English ivy. J. Plant Growth Regul. 10, 215-220 https://doi.org/10.1007/BF02279337
  14. Gill, R., S. Eapen and P. S. Rao. 1987. Callus induction from protoplasts of Vigna unguiculata, V. sublobata and V. Mungo. Theor. Appl. Genet. 74, 100-103 https://doi.org/10.1007/BF00290091
  15. Gladfelter, H. J. and G. C. Phillips. 1987. De novo shoot organogenesis of Pinus eldarica Medw. in vitro. I. Reproductive regeneration from long-term callus cultures. Plant Cell Rep. 6, 163-166 https://doi.org/10.1007/BF00268468
  16. Gonzalez, A., A. Casares, T. R. Sanchez and R. Rodriguez. 1991. Adventitious root induction in Corylus avellana I. cotyledon slices. In Vitro Cell. Dev. Biol. Plant 27, 125-131 https://doi.org/10.1007/BF02632195
  17. Gulati, A. and P. K. Jaiwal. 1992. In vitro induction of multiple shoots and plant regeneration from shoot tips of mung bean (Vigna radiata (L.) Wilczek). Plant Cell. Tiss. Org Cult. 29, 199-205 https://doi.org/10.1007/BF00034353
  18. Gulati, A. and P. K. Jaiwal. 1994. Plant regeneration from cotyledonary node explants of mungbean (Vigna radiata L. Wilczek). Plant Cell Rep. 13, 523-527
  19. Haissig, B. E. 1986. Metabolic processes in adventitious rooting of cuttings, pp. 142-189, In Jackson, M. B. (ed.), New Root Formation in Plant and Cuttings, Martinus Nijhoff Publishers, Boston
  20. Haissig, B. E. 1988. Future directions in adventitious rooting research, pp. 302-310, In Davis, T. D., B. E. Haissig and N. Sankhla (eds.), Adventitious Root Formation in Cutting, Dioscorides Press, Portland, Oregon
  21. Hicks, G. S. 1987. Adventitious rooting of apple micro-cuttings in vitro: an anatomical study. Can. J. Bot. 65, 1913-1920 https://doi.org/10.1139/b87-262
  22. Kaviraj, C. P., G. Kiran, R. B. Venugopal, P. B. Kavikishor and S. Rao. 2006. Somatic embryogenesis and plant regeneration from cotyledonary explants of green gram (Vigna radiata (L.) Wilczek)-a recalcitrant grain legume. In Vitro Cell. Dev. Biol. Plant 42, 134-138
  23. Liau, D. F. and W. G. Boll. 1970. Callus and cell suspension culture of bush bean (Phaseolus vulgaris). Can. J. Bot. 48, 1119-1130 https://doi.org/10.1139/b70-162
  24. Mathews, H. 1987. Morphogenetic responses from in vitro cultured seedling explants of mung bean (Vigna radiata L. Wilczek). Plant Cell Tiss. Org Cult. 11, 233-240 https://doi.org/10.1007/BF00040429
  25. McCown, B. H. 1988. Adventitious rooting of tissue cultured plants, pp. 289-302, In Davis, T. D., B. E. Haissig and N. Sankhla (eds.), Adventitious Root Formation in Cutting, Dioscorides Press, Portland, Oregon
  26. Mendoza, A. B., K. Hattori, T. Nishimura and Y. Futsuhara. 1993. Histological and scanning electron microscopic observations on plant regeneration in mung-bean cotyledon (Vigna radiata (L.) Wilczek) cultured in vitro. Plant Cell Tiss. Org Cult. 32, 137-143 https://doi.org/10.1007/BF00029835
  27. Mundhara, R. and A. Rashid. 2006. Recalcitrant grain legume Vigna radiata, mung bean, made to regenerate on change of hormonal and cultural conditions. Plant Cell Tiss. Org Cult. 85, 265-270 https://doi.org/10.1007/s11240-005-9061-3
  28. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  29. Olieman-van der Merr, A. W., R. L. M. Pierik and S. Ruest. 1971. Effects of sugar, auxin, and light on adventitious root formation in isolated stem explants of Phaseolus and Rhododendron. Meded. Fac. Landbonwwet Rijksunic Genet. 36, 511-518
  30. Perez-Frances, J. F., F. Valdes and R. Martin. 1995. Callus induction and culture from explants of Erysimum scoparium in a growth regulator-free medium. Plant Cell Tiss. Org Cult. 43, 223-228
  31. Peters, J. E., O. J. Crocomo and W. R. Sharp. 1976. Effects of caffeine and nicotine on the callus growth and root morphogenesis of Phaseolus vulgaris tissue cultures. Turrialba. 86, 337-341
  32. Sen, J. and S. Guha-Mukherjee. 1998. In vitro induction of multiple shoots and plant regeneration in Vigna. In Vitro Cell. Dev. Biol. Plant. 34, 276-280 https://doi.org/10.1007/BF02822733
  33. Sterling, C. 1951. Origin of buds in tobacco stem segments cultured in vitro. Amer. J. Bot. 38, 761-767 https://doi.org/10.2307/2438198
  34. Steward, F. C., M. O. Mapes and K. Mears. 1958. Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. Amer. J. Bot. 45, 705-708 https://doi.org/10.2307/2439728
  35. Tivareker, S. and S. Eapen. 2001. High frequency plant regeneration from immature cotyledons of mung bean. Plant Cell Tiss. Org Cult. 66, 227-230 https://doi.org/10.1023/A:1010644812268
  36. Torregrosa, L. and A. Bouquet. 1996. Adventitious bud formation and shoot development from in vitro leaves of Vitis X Muscadinia hybrids. Plant Cell Tiss. Org Cult. 45, 245-252 https://doi.org/10.1007/BF00043637
  37. Torrey, J. G. 1966. The initiation of organized development in plants, pp. 39-91, In Abercrombie, M. and J. Brachet (eds.), Advances in Morphogenesis, Vol. 5, Academic Press, New York
  38. Villalobos, V. M., E. C. Yeung and T. A. Thorpe. 1985. Origin of adventitious shoots in excised Radiata pine cotyledons cultured in vitro. Can. J. Bot. 63, 2172-2176 https://doi.org/10.1139/b85-307
  39. Voesenek, L. A. C. J., C. W. P. M. Blom and R. H. W. Pouwels. 1989. Root and shoot development of Rumex species underwaterlogged conditions. Can. J. Bot. 67, 1865-1869 https://doi.org/10.1139/b89-236
  40. Wagley, L. M., H. J. Gladfelter and G. C. Phillips. 1987. De novo shoot organogenesis of Pinus eldarica Medw. in vitro. II. Macro- and micro-photographic evidence of de novo regeneration. Plant Cell Rep. 6, 167-171 https://doi.org/10.1007/BF00268469