Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Enzymatic Regulatory Effects of Laninaria japonica Fucoidan Extract in Hepatotoxicity

다시마 푸코이단 추출물의 간독성에서 효소 조절 효능에 관한 연구

  • Kang, Kum-Suk (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Nam, Chun-Suk (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) ;
  • Park, Eun-Kyung (Central Research Institute, Binex Co., Ltd.) ;
  • Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
  • 강금석 (신라대학교 의생명과학대학 제약공학과) ;
  • 남천석 (신라대학교 의생명과학대학 제약공학과) ;
  • 박은경 (바이넥스 기업부설연구소) ;
  • 하배진 (신라대학교 의생명과학대학 제약공학과)
  • Published : 2006.12.01
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Abstract

The purpose of this study was to investigated the effects of Laminaria japonica fucoidan extract (LJFE) through the enzymatic regulation against the hepatotoxicity-inducing carbon tetrachloride $(CCl_4)$ in LJFE and $CCl_4-treated$ rats. LJFE of 100mg/kg concentration was intraperitoneally administered into rats at dose of 1.5m11kg for 14 days. On the day 15, 3.3ml/kg of $CCl_4$ dissolved in olive oil (1:1) was injected 12 hours before anesthetization. We examined the levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT) in serum of rats, superoxide dismutase(SOD) in mitochondrial fraction, and catalase(CAT), glutathione peroxidase(GPx) in liver homogenate. $CCl_4-treatment$ markedly increased the levels of GOT and GPT, and significantly decreased those of SOD, CAT and GPx. But LIFE pretreatment decreased the levels of GOT and GPT, by 40% and 64%, respectively and increased those of SOD, CAT and GPx, by 114%, 36.1% and 55.9%, respectively These results showed the LIFE had the enzymatic regulatory effects against the hepatotoxicity-inducing $CCl_4$ in the preventive way.

다시마(Laminaria japonica)는 우리나라 해역에서 생산되는 대표적인 갈조류로 칼슘, 칼륨, 요오드, 아연 등 신체의 생리대사에 관여하는 주요 무기질이 풍부하게 함유되어 있으며, uronic acid로 구성된 alginic acid, 황산기를 함유하는 fucoidan 및 laminaran과 같은 식이섬유도 함유되어 있다. 다시마로부터 추출된 푸코이단의 항 간독성 효과를 확인하고자 흰쥐의 복강 내에 다시마 푸코이단 추출물을 14일간 투여하고 15일째 되는 날에 $CCl_4$를 투여한 후에 혈액 및 간 조직에서의 효소 활성의 변동을 통해 간독성에 대한 효과를 관찰하였다. GOT의 경우 정상군에 비해 대조군에서 $CCl_4$의 간 장애 유발로 인해 효소 level이 약 3.8배 증가하였고 시료군인 LFC군과 대조군을 비교하였을 때 40% 감소하여 다시마 푸코이단 추출물의 효과를 확인할 수 있었다 역시 GPT level에 있어서도 $CCl_4$를 투여한 대조군이 정상군에 대해 약 11.5배정도 증가하여 간 장애 유발이 확인되었고 LFC군이 대조군에 비해 64%로 감소하는 것으로 나타나 다시마 푸코이단 추출물의 효소 level 감소 효과를 확인할 수 있었다. 간 조직에서의 SOD 활성정도는 대조군에서 정상군에 비해서 약 1.79배 정도로 낮게 나타났고 LFC군은 대조군에 비해 114%의 상승효과를 보였다. 또한 CAT의 활성도는 대조군에서 정상군에 비해 약 1.59배 감소를 보였으며 LFC군은 대조군보다 36.1% 높게 나타났다. GPx활성도의 경우 대조군은 정상군에 비해 약 2.5배의 감소율을 보였으며 LFC군은 대조군에 비해서 55.9%의 증가율을 보였다. 이러한 결과는 다시마 푸코이단 추출물이 간독성에서 효소 조절 효능을 가지므로 기능성 식품소재로 활용될 수 있음을 보여 주었다.

Keywords

References

  1. Aebi, H. 1984. Catalase in vitro, Methods. Enzymology 105, 121-126 https://doi.org/10.1016/S0076-6879(84)05016-3
  2. Ashburm, L. L., K. M. Endicott, F. S. Daft and R. D. Little. 1947. The nonportal distribution of trabecule in dietary cirvhosis of mouse and huinea pigs. Am. J. Path. 23, 159
  3. Ayako, Y., Y. Koichi and O. Keiichi. 1992. Iodine distribution in blades of several Laminarias grown in the same sea area. Nippon Suisan Gakkaishi., 58, 1373-1379 https://doi.org/10.2331/suisan.58.1373
  4. Beauchamp, C. and I. Fridovich. 1971. Superoxide dismutase : improved assays and an assay applicable to acrylamide gel. Anal. Biochem. 44, 276-287 https://doi.org/10.1016/0003-2697(71)90370-8
  5. Butler, T. C. 1990. Reduction of carbon tetrachloride in vivo and reduction of carbon tetrachloride and chloroform in vitro by tissues and tissues constituents. J. Pharmacal. Exp. Ther. 134, 311-319
  6. Choi, J. H., J. S. Choi, D. S. Byun and D. S. Yang. 1986. Basic sudies on the development of diet for the treatment of obesity. II. Comparison of the inhibitory effect of algae and crude drug components on obesity. Bull. Korean Fish. Soc. 19, 485-492
  7. Jeong, C. S., K. W. Jung and J. S. Jeong. 1999. Hepatoprotective Effect of Subfractions of Carthamus tinctorius L. Semen on the Reversal of Biotransformation Enzyme Activities in $CCl_4$-induced Hepatotoxic Rats. J. Fd. Hyg Safety 14(2), 172-178
  8. Fred, J., J. Yost and I. Fridovich. 1976. Superoxide and hydrogen peroxide in oxygen damage. Arch. Biochem. Biophys. 175, 514-518 https://doi.org/10.1016/0003-9861(76)90539-7
  9. Fridovich, I. 1986. Biologic effects of the superoxide radicals. Arch. Biochem. Biophysm 247, 1-15 https://doi.org/10.1016/0003-9861(86)90526-6
  10. Gabriel, L. P and R. William, Hewitt. 1982. Hayes : Principles and Methods of Toxicology. Raben Press, pp. 407-445
  11. Gillette, J. R. 1977. Formation of reactive metabolites of foreign compounds and their covalent binding to cellular constituents. American Physiological Society
  12. Gutteridge, J. M. C., A. P. C. Beard and G. J. Quinlan. 1983. Superoxide-dependent lipid peroxidation. Problem with the use of catalase as a specific probe for Fenton-derived hydroxyl radicals. Biochem. Biophys. Res. Commun. 117, 901-907 https://doi.org/10.1016/0006-291X(83)91681-9
  13. Ling, H. R., H. Siren, M. L. Riekkola, P. Vuorela, H. Vuorela and R. Hiltunen. 1996. Optimized separation of pharmacologically active flavonoids from Epimedium species by capillary electrophoresis. Journal of Chromatography A 746, 123-129 https://doi.org/10.1016/0021-9673(96)00287-7
  14. Hwang. S. H, J. I. Kim and C. J. Sung. 1996. Analysis of insoluble(IDF) and soluble dietary fiber (SDF) content of common Korean foods consumed by Korean male college students. J. Korean Soc. Food Sci. Nutr. 29. 278-285
  15. Kim, Y. Y., K. W. Lee, G. B. Kim and Y. K. Cho. 2000. Studies on physicohemical acd biological properties of depolymerized alginate from sea tangle, Laminaria japonicus by thermal decomposition. J. Korean Fish. Soc. 33, 388-392
  16. Lahaye, M. 1991. Marine algae as sources of fibers contents in some sea vegetable. J. Sci. Food Agric. 54, 587-594 https://doi.org/10.1002/jsfa.2740540410
  17. Lawrence, R. A and R. F. Burk. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem. Biophys. Res. Commun. 71, 952-958 https://doi.org/10.1016/0006-291X(76)90747-6
  18. Lee, K. S., J. S. Seo and Y. S. Choi. 1998. Effect of sea tangle and hypoglycemic agent on lipid metabolism in diabetic rats. J. Korean Soc. Food Sci. Nutr. 27, 960-967
  19. Lowry, O. H., N. J. Rosenbrough, A. S. Farr and R. J. Randall. 1951. Protein Measurenment with Folin phenol reagent. J. Bioi. Chem. 193, 256-261
  20. McCay, P. B., E. K. Lai, J. L. Poyer, C. M. Dubose and E. G. Janzen. 1984. Oxygen and carbon-centered free radical formation during carbon tetrachloride metabolism. J. Biol. Chem. 259, 2135-2143
  21. McPhalen, C. A., M. G. Vincent and J. N. Jansonius. 1992. X-ray structure refinement and comparison of three forms of mitochondrial aspartate aminotransferase. J. Mol. Biol. 225, 495-517 https://doi.org/10.1016/0022-2836(92)90935-D
  22. Nakashme, H., Y. Kido, N. Kobayashi, N. Motoki, M. Neushal and N. Yamamoto. 1987. Purification and characterization of an avian Myeloblastosis and human immunodeficiency virus reverse transcriptase inhibitor sulfated polysaccharide extracted from sea tangle. Antimicrob. Agents Chemother. 31, 1524-1530 https://doi.org/10.1128/AAC.31.10.1524
  23. Oh, C. K., M. C. Oh, S. H. Kim, S. B. Rhim and S. H. Kim. 1998. Antimutagenic and antimicrobial effect of ethanol extracts from sea-mustard and sea tangle. J. Korean Fish. Soc. 31, 90-94
  24. Rosen, D. R., M. Jakobisiak, R. Hartmann-Petersen, S. Ortega, D. J. Johnston. 1993. Mutations with familial amyotrophic lateral sclerosis. Nature 362, 59-62 https://doi.org/10.1038/362059a0
  25. Ryu, B. H., D. S. Kim, K. K. Cho and D. B. Shin. 1989. Antitumer activity of seaweeds toward sarcoma-180. Korean J. Food Sci. Technol. 21, 595-600
  26. Tainer, J. A., E. D. Getzoff, J. S. Richardson and D. C. Richardson. 1983. Structure and mechanism of Cu, Zn, superoxide dismutase. Nature 306, 274-287 https://doi.org/10.1038/306274a0
  27. Takaharu, N and Kiyonori, Y. 1999. Low-dose ${\gamma}$ ray irradiation reduces oxidative damage induced by CCW in mouse liver. Free Radical Biology Medicine 27(11/12), 1324-1333 https://doi.org/10.1016/S0891-5849(99)00180-X
  28. Tako, M., M. Uehara, Y. Kawashima, I. Chinen and F. Hongo. 1996. Isolation and identification of fucoidan from Okinawamozuku. Oyo Toshitsu Kagaku. J. Appl. Glycosci. 43, 143-148
  29. Von, Sonntag. 1987. In 'The Chemical Basis of Radiation of biology' Tylor and Francis.(ed.). London. pp. 31
  30. Yosjikawa, T., M. Murakami, N. Yoshida, O. Seto and M. Kondo. 1983. Effects of superoxide dimutase and catalase on disseminated intravascular coagulation in rats. Thromb. Haenwstas 50, 869-872
  31. 조명행, 2004 : 기초 독성학 P 116-120

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