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Morphological Characteristics and Molecular Identification of Calonectria ilicicola Causing Soybean Red Crown Rot

콩 검은뿌리썩음병균(Calonectria ilicicola)의 형태적 특징과 유전자 수준에서의 동정

  • Park, Seong-Woo (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim, Hong-Sik (Department of Crop Science, College of Agriculture, Life and Environment Sciences Chungbuk National University) ;
  • Woo, Sun-Hee (Department of Crop Science, College of Agriculture, Life and Environment Sciences Chungbuk National University) ;
  • Shim, Heong-Kwon (Honam Agricultural Research Institute) ;
  • Kim, Heung-Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 박성우 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학) ;
  • 김홍식 (충북대학교 농업생명환경대학 식물자원생산학부 식물자원학) ;
  • 우선희 (충북대학교 농업생명환경대학 식물자원생산학부 식물자원학) ;
  • 심형권 (호남농업연구소 식물환경과) ;
  • 김흥태 (충북대학교 농업생명환경대학 응용생명환경학부 식물의학)
  • Published : 2006.12.01

Abstract

The 18 isolates of soybean red crown rot pathogen(Calonectria ilicicola) were isolated from infected soybean plants showing chlorosis symptom and red perithecia sign in 2002 and 2003 season in Korea. The morphological characteristics of the typical isolate SC03-15, such as macroconidia and macrovesicles in anamorph as well as perithecia, asci and ascospores in teleomorph, were observed from red crown rot pathogens. The perithecia were red-brown globose to ovoid, and $357.9(312.5{\sim}391.5.7){\mu}m$ high and $474.3(419{\sim}535){\mu}m$ diameter. Asci were hyaline and clavate and included 8 ascospores, which were straight to slightly curved, 1(-3)-septate, and $36.9(31.1{\sim}49.4){\times}5.2(4.5{\sim}6.1){\mu}m$. Macroconidia in anamorph were cylindrical, hyaline and in size $73.2(68.1{\sim}77.6){\times}5.8(4.9{\sim}6.8){\mu}m$. Further identification process was performed through molecular method, such as a phylogenetic analysis by rDNA sequencing of ITS and $\beta$-tubuline genes of all isolates tested in this study. All isolates were identified as Calonectria ilicicola by DNA sequencing of ITS and $\beta$-tubuline genes, and morphological characteristics.

2002년과 2003년 중부 이남의 콩 재배지에서 지상부에 황화 증상을 보이며, 지제부에 붉은 색의 자낭각이 형성된 콩을 채집하여 검은뿌리썩음병균을 분리하였다. 분리한 병원균으로부터 유성세대의 자낭각, 자낭, 자낭포자를, 무성세대의 분생포자와 소낭을 관찰하였다. 자낭각의 크기는 $357.9(312.5{\sim}391.5.7){\times}474.3(419{\sim}535){\mu}m$로 적갈색의 구형 또는 난형이었으며, 자낭(크기: $90.9{\times}15.0{\mu}m$)은 무색투명한 곤봉상의 모양을 나타내고 있었다. 자낭안에 8개의 자낭포자가 형성되었는데, 무색투명하며 1-3개의 격벽을 지닌 약간 굽은 긴 형태로 크기는 $36.87(31.1{\sim}49.4){\times}5.22(4.5{\sim}6.1){\mu}m$이었다. 무성생식세대에서 만드는 분생포자는 긴 원통형이며 색깔은 투명하고, 크기는 $73.24(68.1{\sim}77.6){\times}5.76(4.9{\sim}6.8){\mu}m$로 자낭포자보다 컸다. 소낭의 모양은 둥근 모양이며 크기는 지름이 $8.46(6.2{\sim}10.0){\mu}m$로 관찰되었으며, 이는 Crous의 결과와 일치하였다. ITS와 $\beta$-tubuline 유전자의 염기서열을 조사하여 phylogenetic analysis를 수행한 결과, 국내에서 분리한 18개의 병원균과 일본에서 분양받은 10개의 병원균 모두 Cylindrocladium parasiticum으로 동정되었다.

Keywords

References

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